NHA_Phlebotomy_Certification_Exam_Study_Guide_
2025_2026
1. 1. What temperature should a semen sample be trans- 36-38 degrees C
ported?
2. 2. Which of the following would result in a grossly b. Incorrect needle gauge
hemolyzed specimen?
a. Incorrect order of draw
b. Incorrect needle gauge
c. Applying tourniquet too close to the draw site
d. Failure to invert tube
3. 3. Which of the following is proper procedure after d. Place specimen in ice-
collecting ammonia specimen? water slurry (or a cup of
a. Protect from light ice)
b. Keep specimen warm
c. Keep specimen at body temp
d. Place specimen in icewater slurry (or a cup of ice)
4. 4. The phlebotomist has a request to collect a CBC a. Either heel
from a healthy 10 month old. Which is appropriate?
5. 5. A phlebotomist is preparing to draw blood from a a. Implied consent
patient in hospital/ The patient extends his arm and
turns head away. Which consent does this indicate?
6. 6. Why should phlebotomist document volume of a. Iatrogenic anemia could
blood drawn from a patient? be a result fo too much
taken
7. 7. A phlebotomist has a requisition to draw blood on a. Speak with the patient
a patient with severe Down's syndrome. What steps guardian before perform-
would you take? ing venipuncture to en-
msure the patient under-
stands
,8. 8. A 69 year old unconscious patient is brought ot the a. Implied consent
ER. Several blood tests are ordered. The phlebotomist
collects blood under
9. 9. Which of the following actions is appropriate when a. Label specimens at the
labeling specimens? time of collection in front
of the patient
10. 10. Before collecting blood on an inpatient, the phle- a. Don't draw until nurse
botomist notices there's no wristband on wrist. There puts an armband on pa-
is one on the IV pole. What do you do? tient
11. 11. Which is appropriate form of identi�cation in inpa- a. Verbal and wrist band
tient setting?
12. 12. WBC, RBC, Electrolytes are ordered. Which tubes a. Lavender and red
are used for capillary?
13. 13. A blood specimen is rejected by lab with QNS as a. The specimen was insuf-
the reason? Why? �cient-not enough blood
14. 14. Which of the following is best method to prevent a. Apply pressure to pre-
internal or external bleeding from venipuncture site vent leakage of blood into
after needle is removed? the tissues
15. 15. A physician orders a STAT but phlebotomist for- a. Lab rejects the misla-
gets to �ll out patient ID number. What is appropriate beled specimen
procedure for an improperly labeled specimen when
received in lab?
16. 16. Phlebotomist is performing venipuncture and pa- a. Discontinue the draw
tient begins to seize. What is correct action?
,17. 17. Phlebotomist receives orders to draw an infant a. O2 levels
screening card, O2 levels, bilirubin, and DNA. Which
is to be drawn �rst?
18. 18. When performing a venipuncture on a patient of a. Insert until a change in
average weight, which is the correct technique for resistance
needle insertion?
19. 19. Which is the best method to collect a blood speci- a. Choose a syringe and
men from an older adult patient who has fragile, easy butter y assembly
to collapse veins?
20. 20. Which of the following is the term for donation of a. Autologous donation
a patient's own blood for an upcoming surgery?
21. 21. The order of draw for capillary collections is di er- a. Increased risk of
ent than venipuncture because coagulation (remember
platelets start to form)
22. 22. Upon entering a patient's room for routine blood a. Return later after draw-
draw, phlebotomist notices clergy member is with the ing other patients
patient. What should you do?
23. 23. A phlebotomist notices a hematoma developing at a. Release the tourniquet
the site of venipuncture. The phlebotomist should and stop the draw
24. 24. Know ORDER of DRAW!!
25. 25. A phlebotomist working in an infertility clinic and a. As soon as you collect
the patient is to submit a semen specimen. The phle- the specimen, keep it next
botomist would tell the patient: to your body and bring it
in right away. (the reason
is it needs to stay at body
, temp and delivered in lab
within 2 hours)
26. 26. After placing tourniquet on a patients arm, a. Petechiae
the phlebotomist notices small red spots below the
tourniquet.
27. 27. The phlebotomist should examine the antecubital a. The median cubital
veins of both arms because: might be more accessible
on one side than the other
28. 28. What is the appropriate cleanser to clean the mea- a. Benzalkonium chloride
tus for a clean catch urine specimen?
29. 29. When should the code on glucose cuvettes or a. When the patient is test-
strips be compared to the code on the glucose meter? ed
30. 30. Which of the following is appropriate to use when a. SST (this is the red gel
collecting for a chemistry test that requires serum? tube)
31. 31. A patient state she is a di cult draw and requests a. Use a syringe and trans-
a skin puncture. The test is a PTT. Which should you fer to a light blue tube
do?
32. 32. Industry standard states needle insertion in the a. 15-30 degree angle
antecubital area should be:
33. 33. Tubes with which additive should be used for CBC? a. EDTA (purple tube)
34. 34. When preparing for transport, which specimen a. Arterial Blood Gases
must be placed on ice within 30 minutes after collec-
tion?
35. a. N-95 respirator mask
2025_2026
1. 1. What temperature should a semen sample be trans- 36-38 degrees C
ported?
2. 2. Which of the following would result in a grossly b. Incorrect needle gauge
hemolyzed specimen?
a. Incorrect order of draw
b. Incorrect needle gauge
c. Applying tourniquet too close to the draw site
d. Failure to invert tube
3. 3. Which of the following is proper procedure after d. Place specimen in ice-
collecting ammonia specimen? water slurry (or a cup of
a. Protect from light ice)
b. Keep specimen warm
c. Keep specimen at body temp
d. Place specimen in icewater slurry (or a cup of ice)
4. 4. The phlebotomist has a request to collect a CBC a. Either heel
from a healthy 10 month old. Which is appropriate?
5. 5. A phlebotomist is preparing to draw blood from a a. Implied consent
patient in hospital/ The patient extends his arm and
turns head away. Which consent does this indicate?
6. 6. Why should phlebotomist document volume of a. Iatrogenic anemia could
blood drawn from a patient? be a result fo too much
taken
7. 7. A phlebotomist has a requisition to draw blood on a. Speak with the patient
a patient with severe Down's syndrome. What steps guardian before perform-
would you take? ing venipuncture to en-
msure the patient under-
stands
,8. 8. A 69 year old unconscious patient is brought ot the a. Implied consent
ER. Several blood tests are ordered. The phlebotomist
collects blood under
9. 9. Which of the following actions is appropriate when a. Label specimens at the
labeling specimens? time of collection in front
of the patient
10. 10. Before collecting blood on an inpatient, the phle- a. Don't draw until nurse
botomist notices there's no wristband on wrist. There puts an armband on pa-
is one on the IV pole. What do you do? tient
11. 11. Which is appropriate form of identi�cation in inpa- a. Verbal and wrist band
tient setting?
12. 12. WBC, RBC, Electrolytes are ordered. Which tubes a. Lavender and red
are used for capillary?
13. 13. A blood specimen is rejected by lab with QNS as a. The specimen was insuf-
the reason? Why? �cient-not enough blood
14. 14. Which of the following is best method to prevent a. Apply pressure to pre-
internal or external bleeding from venipuncture site vent leakage of blood into
after needle is removed? the tissues
15. 15. A physician orders a STAT but phlebotomist for- a. Lab rejects the misla-
gets to �ll out patient ID number. What is appropriate beled specimen
procedure for an improperly labeled specimen when
received in lab?
16. 16. Phlebotomist is performing venipuncture and pa- a. Discontinue the draw
tient begins to seize. What is correct action?
,17. 17. Phlebotomist receives orders to draw an infant a. O2 levels
screening card, O2 levels, bilirubin, and DNA. Which
is to be drawn �rst?
18. 18. When performing a venipuncture on a patient of a. Insert until a change in
average weight, which is the correct technique for resistance
needle insertion?
19. 19. Which is the best method to collect a blood speci- a. Choose a syringe and
men from an older adult patient who has fragile, easy butter y assembly
to collapse veins?
20. 20. Which of the following is the term for donation of a. Autologous donation
a patient's own blood for an upcoming surgery?
21. 21. The order of draw for capillary collections is di er- a. Increased risk of
ent than venipuncture because coagulation (remember
platelets start to form)
22. 22. Upon entering a patient's room for routine blood a. Return later after draw-
draw, phlebotomist notices clergy member is with the ing other patients
patient. What should you do?
23. 23. A phlebotomist notices a hematoma developing at a. Release the tourniquet
the site of venipuncture. The phlebotomist should and stop the draw
24. 24. Know ORDER of DRAW!!
25. 25. A phlebotomist working in an infertility clinic and a. As soon as you collect
the patient is to submit a semen specimen. The phle- the specimen, keep it next
botomist would tell the patient: to your body and bring it
in right away. (the reason
is it needs to stay at body
, temp and delivered in lab
within 2 hours)
26. 26. After placing tourniquet on a patients arm, a. Petechiae
the phlebotomist notices small red spots below the
tourniquet.
27. 27. The phlebotomist should examine the antecubital a. The median cubital
veins of both arms because: might be more accessible
on one side than the other
28. 28. What is the appropriate cleanser to clean the mea- a. Benzalkonium chloride
tus for a clean catch urine specimen?
29. 29. When should the code on glucose cuvettes or a. When the patient is test-
strips be compared to the code on the glucose meter? ed
30. 30. Which of the following is appropriate to use when a. SST (this is the red gel
collecting for a chemistry test that requires serum? tube)
31. 31. A patient state she is a di cult draw and requests a. Use a syringe and trans-
a skin puncture. The test is a PTT. Which should you fer to a light blue tube
do?
32. 32. Industry standard states needle insertion in the a. 15-30 degree angle
antecubital area should be:
33. 33. Tubes with which additive should be used for CBC? a. EDTA (purple tube)
34. 34. When preparing for transport, which specimen a. Arterial Blood Gases
must be placed on ice within 30 minutes after collec-
tion?
35. a. N-95 respirator mask
