BIOC384: Exam 1 UPDATED Exam
Questions and CORRECT Answers
Micelles - CORRECT ANSWER - lipid monolayer structure
Liposomes - CORRECT ANSWER - lipid bilayer structure
2 properties of membranes - CORRECT ANSWER - lateral mobility/diffusion and
transverse flipping (flippases speed it up)
3 major classes of membranes - CORRECT ANSWER - plasma membrane,
mitochondrial/chloroplast membrane and endomembrane system
TPS: Explain why the bleached dot disappears over time - CORRECT ANSWER - lateral
diffusion of proteins in the membrane- determine rate of movement
TPS: How could this experiment help characterize a newly discovered membrane receptor? -
CORRECT ANSWER - rate of lateral protein diffusion can be determine- if movement of
new protein is slower it could indicate it is anchored or constrained in the membrane
Hydrogen bonds provide __________ within the DNA double helix - CORRECT
ANSWER - specificity
Base stacking provides _________ within the DNA double helix - CORRECT ANSWER -
stability via hydrophobic effect
T or F. G/C interactions are more stable than A/T ones - CORRECT ANSWER - true
What is the melting temperature (Tm)? - CORRECT ANSWER - The Tm is the
temperature at which half of the DNA molecules are denatured and half are folded
, TPS: Why does base-stacking contribute more to stability than hydrogen bonding? - CORRECT
ANSWER - H-bond energy is equal in ss and ds form, but ds is favored so stability must
be due to energy provided by base stacking
TPS: explain why uracil is not found in DNA? - CORRECT ANSWER - cytosine
deamination in DNA generates uracil but DNA repair enzymes remove it- T has to pair with A in
DNA- U never found in DNA
What are some proteins that bind to DNA in a sequence-independent manner? - CORRECT
ANSWER - Histone proteins and single-stranded binding proteins
What is a protein that binds to DNA in a sequence-dependent manner? - CORRECT
ANSWER - Lac repressor in E. Coli
what are 4 primary methods used in nucleic acid biochemistry? - CORRECT ANSWER -
DNA sequencing, PCR analysis, recombinant DNA, and gene expression analysis
What is special about eukaryotic genes? - CORRECT ANSWER - Alternative splicing; it
is caused by the presence of different splice sites within the intron-exon junction- different
proteins
What are some causes of differences in 2 people's DNA? - CORRECT ANSWER -
polymorphisms, insertions, or deletions
3 ways DNA can be transferred between organisms - CORRECT ANSWER - conjugation,
transformation and transduction
What are some other nucleic acid methods? - CORRECT ANSWER - Gene cloning, DNA
sequencing, PCR and transcriptome analysis
Questions and CORRECT Answers
Micelles - CORRECT ANSWER - lipid monolayer structure
Liposomes - CORRECT ANSWER - lipid bilayer structure
2 properties of membranes - CORRECT ANSWER - lateral mobility/diffusion and
transverse flipping (flippases speed it up)
3 major classes of membranes - CORRECT ANSWER - plasma membrane,
mitochondrial/chloroplast membrane and endomembrane system
TPS: Explain why the bleached dot disappears over time - CORRECT ANSWER - lateral
diffusion of proteins in the membrane- determine rate of movement
TPS: How could this experiment help characterize a newly discovered membrane receptor? -
CORRECT ANSWER - rate of lateral protein diffusion can be determine- if movement of
new protein is slower it could indicate it is anchored or constrained in the membrane
Hydrogen bonds provide __________ within the DNA double helix - CORRECT
ANSWER - specificity
Base stacking provides _________ within the DNA double helix - CORRECT ANSWER -
stability via hydrophobic effect
T or F. G/C interactions are more stable than A/T ones - CORRECT ANSWER - true
What is the melting temperature (Tm)? - CORRECT ANSWER - The Tm is the
temperature at which half of the DNA molecules are denatured and half are folded
, TPS: Why does base-stacking contribute more to stability than hydrogen bonding? - CORRECT
ANSWER - H-bond energy is equal in ss and ds form, but ds is favored so stability must
be due to energy provided by base stacking
TPS: explain why uracil is not found in DNA? - CORRECT ANSWER - cytosine
deamination in DNA generates uracil but DNA repair enzymes remove it- T has to pair with A in
DNA- U never found in DNA
What are some proteins that bind to DNA in a sequence-independent manner? - CORRECT
ANSWER - Histone proteins and single-stranded binding proteins
What is a protein that binds to DNA in a sequence-dependent manner? - CORRECT
ANSWER - Lac repressor in E. Coli
what are 4 primary methods used in nucleic acid biochemistry? - CORRECT ANSWER -
DNA sequencing, PCR analysis, recombinant DNA, and gene expression analysis
What is special about eukaryotic genes? - CORRECT ANSWER - Alternative splicing; it
is caused by the presence of different splice sites within the intron-exon junction- different
proteins
What are some causes of differences in 2 people's DNA? - CORRECT ANSWER -
polymorphisms, insertions, or deletions
3 ways DNA can be transferred between organisms - CORRECT ANSWER - conjugation,
transformation and transduction
What are some other nucleic acid methods? - CORRECT ANSWER - Gene cloning, DNA
sequencing, PCR and transcriptome analysis
