, period of DNA synthesis = Viral Capsid Ag (EBV-VCA) 3) Latent Phase Ags: a. EBV Nuclear Ag (EBNA) and
b. Latent Membrane Proteins (LMP's) not as important.
Describe symptoms and disease course for EBV clinical illness (infectious mononucleosis): - 1) Spreads:
oral transmission via droplets 2) benign self-limiting acute illness 3) symptoms: fever, sore throat,
lymphadenopathy (swollen lymph nodes) 4) some develop chronic infection >6mths 5) Virus can be
reactivated after latency (chronic fatigue) 6) EBV associated w/ malignancies
What are the malignancies that have been associated w/ EBV? - 1) Hematologic: Burkitt's lymphoma &
Hodgkin's disease 2) Nonhematologic: nasopharyngeal & gastric carcinoma 3) lymphoproliferative
disorder in immunocompromised: organ transplants & AIDS, primary immunodeficiency
Lab Diagnosis of EBV: - 1) hemotologic: High WBC counts, proliferative/atypical or reactive lymphocytes
2) Serologic: presence of heterophile Ab & Ab to certain EBV Ag (nuclear & viral capsid)
Name the 2 rapid tests that use Heterophile Abs? - 1) Monospot: tests ability of patient serum absorbed
w guinea pig,cow,kidney RBC Ag to agglutinate horse RBCs. Lacks specificity but widely used.
2) Wampole Mono-Latex: LA slide test for qual/semi-qual detection of IM heterophile Abs in serum or
plasma (some False+s w/ pts. have lymphoma,viral Hep, AI disease & neg test doesn't rule out IM!)
What types of serological confirmation testing is available for EBV and what does it detect? - • Ab can be
detected by indirect immunofluorescence, ELISA, or immunoblot. • Detects Abs to viral capsid Ag (VCA):
1) Anti-VCA IgM in acute stage (disappears after 2-4mths) 2) Anti-VCA IgG (appears w/in 4-7d of onset &
persists indefinitely)
What is detected serologically for pediatric and transplant patients? - • Ab to Early Ag (EA): 1) EA-
D=acute infection, titer may ↑ in reactivity 2) EA-R=acute infection in young children & transiently in
convalescent stage (monitoring Ag)
In addition to EA-R, what Ab is also associated with convalescent phase & appears along with what other
Ab? - Ab to EB nuclear Ag (EBNA) and appears along w/ Anti-VCA IgG
EBV serology in IM: chart - Anti-VCA (IgM) Anti-VCA (IgG) Anti-EA Anti-EBNA
Acute + + + -
b. Latent Membrane Proteins (LMP's) not as important.
Describe symptoms and disease course for EBV clinical illness (infectious mononucleosis): - 1) Spreads:
oral transmission via droplets 2) benign self-limiting acute illness 3) symptoms: fever, sore throat,
lymphadenopathy (swollen lymph nodes) 4) some develop chronic infection >6mths 5) Virus can be
reactivated after latency (chronic fatigue) 6) EBV associated w/ malignancies
What are the malignancies that have been associated w/ EBV? - 1) Hematologic: Burkitt's lymphoma &
Hodgkin's disease 2) Nonhematologic: nasopharyngeal & gastric carcinoma 3) lymphoproliferative
disorder in immunocompromised: organ transplants & AIDS, primary immunodeficiency
Lab Diagnosis of EBV: - 1) hemotologic: High WBC counts, proliferative/atypical or reactive lymphocytes
2) Serologic: presence of heterophile Ab & Ab to certain EBV Ag (nuclear & viral capsid)
Name the 2 rapid tests that use Heterophile Abs? - 1) Monospot: tests ability of patient serum absorbed
w guinea pig,cow,kidney RBC Ag to agglutinate horse RBCs. Lacks specificity but widely used.
2) Wampole Mono-Latex: LA slide test for qual/semi-qual detection of IM heterophile Abs in serum or
plasma (some False+s w/ pts. have lymphoma,viral Hep, AI disease & neg test doesn't rule out IM!)
What types of serological confirmation testing is available for EBV and what does it detect? - • Ab can be
detected by indirect immunofluorescence, ELISA, or immunoblot. • Detects Abs to viral capsid Ag (VCA):
1) Anti-VCA IgM in acute stage (disappears after 2-4mths) 2) Anti-VCA IgG (appears w/in 4-7d of onset &
persists indefinitely)
What is detected serologically for pediatric and transplant patients? - • Ab to Early Ag (EA): 1) EA-
D=acute infection, titer may ↑ in reactivity 2) EA-R=acute infection in young children & transiently in
convalescent stage (monitoring Ag)
In addition to EA-R, what Ab is also associated with convalescent phase & appears along with what other
Ab? - Ab to EB nuclear Ag (EBNA) and appears along w/ Anti-VCA IgG
EBV serology in IM: chart - Anti-VCA (IgM) Anti-VCA (IgG) Anti-EA Anti-EBNA
Acute + + + -
