Molecular Biology - Molecular Techniques
UPDATED ACTUAL Questions and
CORRECT Answers
The resolution of DNA on an agarose gel - CORRECT ANSWER - Smaller size of DNA
can be resolved as the concentration of agarose increases
Detect RFLP (Restiction fragment length polymorphism) - CORRECT ANSWER -
Southern blot
Reverse dot blot - CORRECT ANSWER - Attaching multiple probes to nitrocellulose
membrane and then hybridizing a single labeled patient sample to multiple probes
The highest stringency for washing a Southern blot after hybridization - CORRECT
ANSWER - High temperature, low salt concentration
The use of the Klenow fragment of E.coli DNA polymerase I - CORRECT ANSWER - To
label DNA probes by random primer labeling
A solution hybridization method for the detection of nucleic acid:protein interactions -
CORRECT ANSWER - Gel mobility shift assay
The probe used to detect trisomy 21 by interphase FISH - CORRECT ANSWER - Alpha
satellite DNA probe
Ethanol volumes added to 1 volume of a DNA:salt solution to case DNA precipitate -
CORRECT ANSWER -2
Chelex 100 resom - CORRECT ANSWER - Extract DNA from cells
UPDATED ACTUAL Questions and
CORRECT Answers
The resolution of DNA on an agarose gel - CORRECT ANSWER - Smaller size of DNA
can be resolved as the concentration of agarose increases
Detect RFLP (Restiction fragment length polymorphism) - CORRECT ANSWER -
Southern blot
Reverse dot blot - CORRECT ANSWER - Attaching multiple probes to nitrocellulose
membrane and then hybridizing a single labeled patient sample to multiple probes
The highest stringency for washing a Southern blot after hybridization - CORRECT
ANSWER - High temperature, low salt concentration
The use of the Klenow fragment of E.coli DNA polymerase I - CORRECT ANSWER - To
label DNA probes by random primer labeling
A solution hybridization method for the detection of nucleic acid:protein interactions -
CORRECT ANSWER - Gel mobility shift assay
The probe used to detect trisomy 21 by interphase FISH - CORRECT ANSWER - Alpha
satellite DNA probe
Ethanol volumes added to 1 volume of a DNA:salt solution to case DNA precipitate -
CORRECT ANSWER -2
Chelex 100 resom - CORRECT ANSWER - Extract DNA from cells
