ASCP CG Certification 2025 LATEST EXAM/ COMPLETE 170 ACTUAL
QUESTIONS WITH CORRECT VERIFIED ANSWERS GRADED A+
1. What is the best agent to use against my- BM cyclin - a combination of
coplasma? two antibiotics (tiamulin and
minocycline) that effecticely
targets mycoplasma growth
2. For successful harvest, each new batch of comparing the new lot to a
hypotonic solution should be evaluated by: lot already in use
3. Colagenase, pronase, and trypsin are: Proteolytic enzymes - break
down tissues into single-cell
suspensions
4. When harvesting peripheral blood cultures, Colcemid - mitotic spindle
asceptic technique no longer matters fol- poison that halts cells at
lowing incubation with: metaphase
5. Samples that arrive at the cytogenetics lab Logged in to the system
should first be:
6. Phytohemagglutinin (PHA) stimulates T-cell lymphocytes
which type of cells into division?
7. To avoid culture failure, monitoring Expiration dates of reagents
reagents is crucial. When logging a culture used
failure, what is the MOST important to doc-
ument?
8. Which type of microscope is used when Dissection
cleaning a CVS (chorionic villus) sample?
9. Which cell type is most problematic if found Decidua (maternal tissue)
in a CVS (chorionic villus) sample?
10. When looking at a slide under a phase con- innappropriate slide drying
trast scope and a lot of cytoplasm is still time
seen around the mets, what is the most like-
ly cause?
, ASCP CG Certification 2025 LATEST
11. What will help prevent microbial contamina- Asceptic technique
tion and cross contamination of cultures?
12. When there are not enough mets to com- prepare additional slides
plete the study of a patient/case, what needs from any remaining sample
to be done next?
13. When looking at a dropped slide under a Increading the drying time by
phase scope and mets are very spread out using warm slides
and sparse, what could help fix the issue?
14. What is true of an inverted microscope? the objectives are beneath
the stage
15. High-resolution banding is indicated for a DiGenorge syndrome -- mi-
possible diagnosis of: crodeletion of 22q11, also
called velocardiofacial syn-
drome
"velo" - soft palette,
"cardio" - congenital heart
defects,
"facial" - unique abnormal fa-
cial features, short philtrum
(upper lip indent), tubular
nose, wide spread eyes, etc
16. When verifying if a specimen is appropriate Reason for referral
for cytogenetic analysis, what is the MOST
important data to accompany the speci-
men?
17. Which type of specimen should be request- Heparinized peripheral
ed for a cytogenetic analysis for turner syn- blood stored at room temper-
drome? ature
(NOT FROZEN)
, ASCP CG Certification 2025 LATEST
18. A blood sample can be used to study the Dividing leukemic cells are
cytogenetics of a malignancy only if: present in circulation
19. All of the cultures in a CO2 incubator failed, Temperature,
what aspects of the incubator are important CO2 level, and
to monitor to assess the source of the prob- Humidity level
lem?
20. The most important factor affecting the Slide drying after the cells
quality of chromosomes viewed under a have been dropped
phase contrast scope is:
21. What is the modal number (default number 45
of chromosomes in a cell) for a Down syn-
drome translocation carrier?
22. What is the best tissue to use for both a Chorionic villi
good success rate and abnormality detec-
tion in spontaneous abortion miscarriages?
23. What is a possible cause for a drying oven The door does not seal prop-
to not maintain a proper temperature? erly
24. If a tech with a cold and sneezing does Mycoplasma
not take proper precautions while setting up
amniotic fluid samples, the specimen could --> the most common cont-
become contaminated with: aminant by a sick tech and
can be detected through a
specific stain used on the
sample
mycoplasma can induce ab-
normalities in a sample, so it
is important to avoid contam-
ination
25. What about the metaphases on a slide are Cell density - thru dilution of
affected by slide making? the sample with fix
QUESTIONS WITH CORRECT VERIFIED ANSWERS GRADED A+
1. What is the best agent to use against my- BM cyclin - a combination of
coplasma? two antibiotics (tiamulin and
minocycline) that effecticely
targets mycoplasma growth
2. For successful harvest, each new batch of comparing the new lot to a
hypotonic solution should be evaluated by: lot already in use
3. Colagenase, pronase, and trypsin are: Proteolytic enzymes - break
down tissues into single-cell
suspensions
4. When harvesting peripheral blood cultures, Colcemid - mitotic spindle
asceptic technique no longer matters fol- poison that halts cells at
lowing incubation with: metaphase
5. Samples that arrive at the cytogenetics lab Logged in to the system
should first be:
6. Phytohemagglutinin (PHA) stimulates T-cell lymphocytes
which type of cells into division?
7. To avoid culture failure, monitoring Expiration dates of reagents
reagents is crucial. When logging a culture used
failure, what is the MOST important to doc-
ument?
8. Which type of microscope is used when Dissection
cleaning a CVS (chorionic villus) sample?
9. Which cell type is most problematic if found Decidua (maternal tissue)
in a CVS (chorionic villus) sample?
10. When looking at a slide under a phase con- innappropriate slide drying
trast scope and a lot of cytoplasm is still time
seen around the mets, what is the most like-
ly cause?
, ASCP CG Certification 2025 LATEST
11. What will help prevent microbial contamina- Asceptic technique
tion and cross contamination of cultures?
12. When there are not enough mets to com- prepare additional slides
plete the study of a patient/case, what needs from any remaining sample
to be done next?
13. When looking at a dropped slide under a Increading the drying time by
phase scope and mets are very spread out using warm slides
and sparse, what could help fix the issue?
14. What is true of an inverted microscope? the objectives are beneath
the stage
15. High-resolution banding is indicated for a DiGenorge syndrome -- mi-
possible diagnosis of: crodeletion of 22q11, also
called velocardiofacial syn-
drome
"velo" - soft palette,
"cardio" - congenital heart
defects,
"facial" - unique abnormal fa-
cial features, short philtrum
(upper lip indent), tubular
nose, wide spread eyes, etc
16. When verifying if a specimen is appropriate Reason for referral
for cytogenetic analysis, what is the MOST
important data to accompany the speci-
men?
17. Which type of specimen should be request- Heparinized peripheral
ed for a cytogenetic analysis for turner syn- blood stored at room temper-
drome? ature
(NOT FROZEN)
, ASCP CG Certification 2025 LATEST
18. A blood sample can be used to study the Dividing leukemic cells are
cytogenetics of a malignancy only if: present in circulation
19. All of the cultures in a CO2 incubator failed, Temperature,
what aspects of the incubator are important CO2 level, and
to monitor to assess the source of the prob- Humidity level
lem?
20. The most important factor affecting the Slide drying after the cells
quality of chromosomes viewed under a have been dropped
phase contrast scope is:
21. What is the modal number (default number 45
of chromosomes in a cell) for a Down syn-
drome translocation carrier?
22. What is the best tissue to use for both a Chorionic villi
good success rate and abnormality detec-
tion in spontaneous abortion miscarriages?
23. What is a possible cause for a drying oven The door does not seal prop-
to not maintain a proper temperature? erly
24. If a tech with a cold and sneezing does Mycoplasma
not take proper precautions while setting up
amniotic fluid samples, the specimen could --> the most common cont-
become contaminated with: aminant by a sick tech and
can be detected through a
specific stain used on the
sample
mycoplasma can induce ab-
normalities in a sample, so it
is important to avoid contam-
ination
25. What about the metaphases on a slide are Cell density - thru dilution of
affected by slide making? the sample with fix
