Microbiology Lab Final Exam with Complete Solutions

Microbiology Lab Final Exam with
Complete Solutions
Give the steps of a PCR Correct Ans-Heat reaction to 95 C to denature DNA

Cool rxn to 55 C to allow primers to anneal

Heat rxn to 72 C to allow Taq polymerase to bind



What machine is used to carry out a PCR reaction? Correct Ans-thermocycler




How many cycles does a PCR rxn usually go? Correct Ans-30




Name the 9 organisms used in the unknown lab Correct Ans-Bacillus subtillis

Enterobacter aerogenes

Entero cloacae

Escherichia coli

Klebsiella pneumoniae

Pseudomonas aeruginosa

Salmonella typhimurium

Staphylococcus aureus

Staphylococcus epidermidis



Which organisms can ferment lactose? Correct Ans-E. coli

, E. cloacae

E. aerogenes

K. pneumonia



Define caliform bacteria Correct Ans-rod-shaped, Gram-negative, non-spore forming, and
motile or non-motile bacteria which can ferment lactose with the production of acid and gas



Name the selective medias used in class Correct Ans-Blood Agar

Bile Esculin

Mannitol Salt Agar (MSA)

MacConkey Agar (MaC)

Eosin Methylene Blue (EMB)

Hektoen Enteric Agar (HEA)

Xylose Lysine Desoxiolate (XLD)



Name the differential medias used in class Correct Ans-O/F Glucose

MRVP

Catalase

Oxidase

Citrate

Starch

Indole

TSI