© EVERLY 2025 ALL RIGHTS RESERVED.
Microbiology Lab Final Exam Questions And
Answers 100% Pass.
All UTA microbiology labs are considered what biosafety level? - Answer✔BSL-2
What are four things that all BSL-2 labs must include? - Answer✔1. PPE
2. Sinks and eye wash stations
3. Restricted access
4. autoclaves (or alternative decontamination methods)
What is the plural for media? - Answer✔medium
media can come in a wide variety of formulations and can exist in what three states? -
Answer✔solid, semi-solid, and liquid
Catalase is found in both - Answer✔prokaryotes and eukaryotes
What is often considered as a virulence factor for many pathogens? - Answer✔coagulase
What large amino acid polymer is found in the vertebrate connective tissues? - Answer✔gelatin
What large amino acid polymer is found in mammalian milk? - Answer✔Casein
A phenol red test has a pH indicator that is what color? - Answer✔red
In gelatin hydrolysis tests media contains what besides gelatin? - Answer✔peptone and beef
extract nutrients
Urea is a nitrogen containing organic molecule and principal end product of - Answer✔protein
catabolism
Durham tube in phenol red test is used to - Answer✔capture gas produced by the microbe
Amylase enzymes are able to cleave starch molecules by breaking what? - Answer✔glycosidic
bonds between glucose monomers
What is the term for water molecules breaking complex molecules into simpler forms? -
Answer✔hydrolysis
, © EVERLY 2025 ALL RIGHTS RESERVED.
E. coli and S. faecalis are considered good sewage indicators because they are not normally -
Answer✔present in water or soil
Are coliforms gram-negative or gram-positive coccus? - Answer✔gram-negative
Microorganisms capable of producing citrate permease will be able to utilize citrate as their -
Answer✔sole carbon source
What is the enzyme that some microorganisms produce that help it grow on Simmons citrate
medium? - Answer✔citrate permease
How do you know if a microorganism is is gelatinase positive? - Answer✔Liquify gelatin
Kovac's reagent reacts with what to produce a red color in SIM test? - Answer✔indole
What percentage of evaporated milk is added to tryptic soy agar for the casein hydrolysis test? -
Answer✔3%
What type of media is Mitis salivarious agar? - Answer✔both selective and differential
Bile Esculin agar media is selective because what compound inhibits growth of gram negative
bacilli and most gram positives? - Answer✔sodium azide
If a microorganism is coagulase positive, clotting of plasma cells can be observed -
Answer✔within one minute
The MR-VP test is performed to determine a microorganism's ability to - Answer✔ferment
glucose and produce by-products
Are anaerobic microbes capable of making catalase? - Answer✔no
What is added to the nitrate reductase test if there is no color change after adding sulfanilic acid
and alpha-naphthylamine? - Answer✔zinc
MacConkey agar is used to isolate - Answer✔gram-negative bacilli
Halotolerant or halophilic bacteria can be isolated using what media? - Answer✔Mannitol Salt
Agar (MSA)
Thiosulfate Citrate Bile Salt (TCBS) agar is selective due to an - Answer✔alkaline environment
Thiosulfate Citrate Bile Salt (TCBS) differentiates species by their ability to - Answer✔ferment
sucrose
What percentage of microbes are considered pathogenic? - Answer✔<1%
TSA is an example of what type of media? - Answer✔undefined
How many biosafety levels are there? - Answer✔4
What phase in the autoclave cycle describes pressure that is slowly released from the chamber
through an exhaust valve until ambient temperature is reached? - Answer✔Exhaust phase
Microbiology Lab Final Exam Questions And
Answers 100% Pass.
All UTA microbiology labs are considered what biosafety level? - Answer✔BSL-2
What are four things that all BSL-2 labs must include? - Answer✔1. PPE
2. Sinks and eye wash stations
3. Restricted access
4. autoclaves (or alternative decontamination methods)
What is the plural for media? - Answer✔medium
media can come in a wide variety of formulations and can exist in what three states? -
Answer✔solid, semi-solid, and liquid
Catalase is found in both - Answer✔prokaryotes and eukaryotes
What is often considered as a virulence factor for many pathogens? - Answer✔coagulase
What large amino acid polymer is found in the vertebrate connective tissues? - Answer✔gelatin
What large amino acid polymer is found in mammalian milk? - Answer✔Casein
A phenol red test has a pH indicator that is what color? - Answer✔red
In gelatin hydrolysis tests media contains what besides gelatin? - Answer✔peptone and beef
extract nutrients
Urea is a nitrogen containing organic molecule and principal end product of - Answer✔protein
catabolism
Durham tube in phenol red test is used to - Answer✔capture gas produced by the microbe
Amylase enzymes are able to cleave starch molecules by breaking what? - Answer✔glycosidic
bonds between glucose monomers
What is the term for water molecules breaking complex molecules into simpler forms? -
Answer✔hydrolysis
, © EVERLY 2025 ALL RIGHTS RESERVED.
E. coli and S. faecalis are considered good sewage indicators because they are not normally -
Answer✔present in water or soil
Are coliforms gram-negative or gram-positive coccus? - Answer✔gram-negative
Microorganisms capable of producing citrate permease will be able to utilize citrate as their -
Answer✔sole carbon source
What is the enzyme that some microorganisms produce that help it grow on Simmons citrate
medium? - Answer✔citrate permease
How do you know if a microorganism is is gelatinase positive? - Answer✔Liquify gelatin
Kovac's reagent reacts with what to produce a red color in SIM test? - Answer✔indole
What percentage of evaporated milk is added to tryptic soy agar for the casein hydrolysis test? -
Answer✔3%
What type of media is Mitis salivarious agar? - Answer✔both selective and differential
Bile Esculin agar media is selective because what compound inhibits growth of gram negative
bacilli and most gram positives? - Answer✔sodium azide
If a microorganism is coagulase positive, clotting of plasma cells can be observed -
Answer✔within one minute
The MR-VP test is performed to determine a microorganism's ability to - Answer✔ferment
glucose and produce by-products
Are anaerobic microbes capable of making catalase? - Answer✔no
What is added to the nitrate reductase test if there is no color change after adding sulfanilic acid
and alpha-naphthylamine? - Answer✔zinc
MacConkey agar is used to isolate - Answer✔gram-negative bacilli
Halotolerant or halophilic bacteria can be isolated using what media? - Answer✔Mannitol Salt
Agar (MSA)
Thiosulfate Citrate Bile Salt (TCBS) agar is selective due to an - Answer✔alkaline environment
Thiosulfate Citrate Bile Salt (TCBS) differentiates species by their ability to - Answer✔ferment
sucrose
What percentage of microbes are considered pathogenic? - Answer✔<1%
TSA is an example of what type of media? - Answer✔undefined
How many biosafety levels are there? - Answer✔4
What phase in the autoclave cycle describes pressure that is slowly released from the chamber
through an exhaust valve until ambient temperature is reached? - Answer✔Exhaust phase
