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Microbiology Lab Practical – Final Exam
Questions And Answers 100% Pass.
Staph epidermidis - Answer✔This is MSA and the side labelled "2" is red. Which Staph does
this indicate?
Staph aureus - Answer✔This is MSA and the side labelled "1" has turned yellow. Which Staph
does this indicate?
Lactose Negative - Answer✔This is an EMB plate. What does the test result of "1" indicate?
Lactose Positive - Answer✔This is an EMB plate. What does the test result of "2" indicate?
E. Coli - Answer✔This is an EMB plate. What is indicated by "3?"
Starch was broken down - Answer✔This is a starch plate. What does the clear boundary around
this colony indicate?
acid was produced - Answer✔Why did this Fermentation Tube turn yellow?
gas was produced - Answer✔What is indicated by the bubble in this Durham Tube inside a
Fermentation Tube?
Protein was not broken down - Answer✔This is a Gelatin Tube Test. In Test Tube 2, it is solid.
What does this mean?
Catalase Test - Answer✔What is the name of this test?
Antibiotic Disk Diffusion (Antibiotic Susceptibility) - Answer✔What is the name of this test?
Mannitol Salt Agar - Answer✔What is the name of this media?
Urea Broth
Urease Enzyme
Tube 1: Positive
Tube 2: Negative - Answer✔What is it testing for?
What does pink indicate?
What does the pale yellow indicate?
, © EVERLY 2025 ALL RIGHTS RESERVED.
Nitrate Reduction - Answer✔What is the name of this test?
neutrophil - Answer✔
lymphocyte - Answer✔
monocyte - Answer✔
eosinophil - Answer✔
basophil - Answer✔
What method is used for the antimicrobial susceptibility test? - Answer✔Kirby-Bauer
What technique is used for the Kirby-Bauer test? - Answer✔swab and make a bacterial lawn
What is the principle of technique for the Kirby-Bauer? - Answer✔disk diffusion
What observations are made in lab with Kirby-Bauer? - Answer✔zone of inhibition
What is reported out to Physicians from the Kirby-Bauer? - Answer✔susceptibility or resistance
What is produced when UV radiation is absorbs by DNA? - Answer✔pyrimidine dimers - which
interferes with DNA replication and transcription
Which bacteria has mechanisms to repair damage from UV radiation? - Answer✔Escherichia
coli
What is a major limitation to UV radiation? - Answer✔It cannot pass through solid surfaces
Why did we use an index card or leave the lid on during the experiment? - Answer✔To act as a
control - we wanted to see growth of bacteria without UV light interaction
What technique was used for UV test? - Answer✔swab and make a bacterial lawn
What kind of radiation is UV? - Answer✔non-ionizing radiation
What type of agar is used for Bacterial Transformation lab? - Answer✔LB - Luria Bertani
What is transformation? - Answer✔When bacteria can pick up DNA from the environment and
make use of the genes it carries
What is GFP? - Answer✔green fluorescent protein - responsible for bioluminescence in jellyfish
Why are we using GFP? - Answer✔to show visual evidence that the DNA transformed with
successful gene expression
What is ARA? - Answer✔arabinose (promoter that activates GFP and Amp resistance genes)
What is the vector used in the transformation lab? - Answer✔pGLO plasmid
What does the amp resistance gene release? - Answer✔Beta lactamase
Microbiology Lab Practical – Final Exam
Questions And Answers 100% Pass.
Staph epidermidis - Answer✔This is MSA and the side labelled "2" is red. Which Staph does
this indicate?
Staph aureus - Answer✔This is MSA and the side labelled "1" has turned yellow. Which Staph
does this indicate?
Lactose Negative - Answer✔This is an EMB plate. What does the test result of "1" indicate?
Lactose Positive - Answer✔This is an EMB plate. What does the test result of "2" indicate?
E. Coli - Answer✔This is an EMB plate. What is indicated by "3?"
Starch was broken down - Answer✔This is a starch plate. What does the clear boundary around
this colony indicate?
acid was produced - Answer✔Why did this Fermentation Tube turn yellow?
gas was produced - Answer✔What is indicated by the bubble in this Durham Tube inside a
Fermentation Tube?
Protein was not broken down - Answer✔This is a Gelatin Tube Test. In Test Tube 2, it is solid.
What does this mean?
Catalase Test - Answer✔What is the name of this test?
Antibiotic Disk Diffusion (Antibiotic Susceptibility) - Answer✔What is the name of this test?
Mannitol Salt Agar - Answer✔What is the name of this media?
Urea Broth
Urease Enzyme
Tube 1: Positive
Tube 2: Negative - Answer✔What is it testing for?
What does pink indicate?
What does the pale yellow indicate?
, © EVERLY 2025 ALL RIGHTS RESERVED.
Nitrate Reduction - Answer✔What is the name of this test?
neutrophil - Answer✔
lymphocyte - Answer✔
monocyte - Answer✔
eosinophil - Answer✔
basophil - Answer✔
What method is used for the antimicrobial susceptibility test? - Answer✔Kirby-Bauer
What technique is used for the Kirby-Bauer test? - Answer✔swab and make a bacterial lawn
What is the principle of technique for the Kirby-Bauer? - Answer✔disk diffusion
What observations are made in lab with Kirby-Bauer? - Answer✔zone of inhibition
What is reported out to Physicians from the Kirby-Bauer? - Answer✔susceptibility or resistance
What is produced when UV radiation is absorbs by DNA? - Answer✔pyrimidine dimers - which
interferes with DNA replication and transcription
Which bacteria has mechanisms to repair damage from UV radiation? - Answer✔Escherichia
coli
What is a major limitation to UV radiation? - Answer✔It cannot pass through solid surfaces
Why did we use an index card or leave the lid on during the experiment? - Answer✔To act as a
control - we wanted to see growth of bacteria without UV light interaction
What technique was used for UV test? - Answer✔swab and make a bacterial lawn
What kind of radiation is UV? - Answer✔non-ionizing radiation
What type of agar is used for Bacterial Transformation lab? - Answer✔LB - Luria Bertani
What is transformation? - Answer✔When bacteria can pick up DNA from the environment and
make use of the genes it carries
What is GFP? - Answer✔green fluorescent protein - responsible for bioluminescence in jellyfish
Why are we using GFP? - Answer✔to show visual evidence that the DNA transformed with
successful gene expression
What is ARA? - Answer✔arabinose (promoter that activates GFP and Amp resistance genes)
What is the vector used in the transformation lab? - Answer✔pGLO plasmid
What does the amp resistance gene release? - Answer✔Beta lactamase
