MICROBIOLOGY LAB FINAL EXAM
WITH ALL QUESTIONS AND ALL
ANSWERS GIVEN
According bto bthe blab bstandard boperating bprocedures, byou bmust bwash byour
bhands bwith bsoap band bwater bfor bat bleast b_________ bbefore bleaving bthe blab.
A)
2 bseconds
B)
5 bseconds
C)
15 bseconds
D)
30 bseconds
E)
1 bminute b- b bcorrect banswer- bC) b
15 bseconds
Which bof bthe bfollowing bstatements bdoes bNOT bcorrectly bdescribe ba brule bthat
byou bmust bfollow bin bthe bmicrobiology blab?
A)
There bis bno beating bor bdrinking ballowed bin bthe bmicrobiology blab.
B)
If byou bspill ba bbacterial bculture bon byour blab bcoat, byou bmust breport bit bto byour
bdemonstrator.
C)
You bmust bdisinfect byour bworkbench bbefore band bafter beach bmicrobiology blab.
D)
No bpersonal beffects bare ballowed bin bthe blab. bYou bmust bfind bsomewhere belse bto
bleave byour bwinter bjacket, btextbooks, bbackpacks betc. bwhile byou battend bthe blab.
E)
,None bof bthe babove. b(All bof bthe babove bstatements bcorrectly bdescribe ba brule
bthat bmust bbe bfollowed bin bthe bmicrobiology blab) b- b bcorrect banswer- bE)
None bof bthe babove. b(All bof bthe babove bstatements bcorrectly bdescribe ba brule
bthat bmust bbe bfollowed bin bthe bmicrobiology blab)
Which bof bthe bfollowing bstatements bcorrectly bdescribe bthe blab bcoat bpolicy bin
bplace bin bthe bmicrobiology blab?
A)
You bmust bwear ba bbuttoned blab bcoat bat ball btimes bwhen bworking bin bthe
bmicrobiology blab.
B)
Between blabs byour blab bcoat bshould bbe bwashed bseparately bfrom bother bclothes
bwith bdetergent band bbleach.
C)
When byou btake byour blab bcoat bhome bto bwash bit byou bshould bcarry bit bin ba
bplastic bbag bto bkeep bit bseparate bfrom byour bother bbelongings.
D)
Your blab bcoat bcannot bbe bworn bin bany bother blab buntil bit bhas bbeen bwashed.
E)
All bof bthe babove bstatements bare btrue. b- b bcorrect banswer- bE)
All bof bthe babove bstatements bare btrue.
Which bof bthe bfollowing bstatements bbest bexplains bwhy byou bshould bnever bwear
byour bmicrobiology blab bcoat bin bthe bhallways, beither bbefore bor bafter ba blab?
b(Even bif bit's bclean.)
A)
Because byour blab bcoat bis blikely bto bbe bcontaminated bwith bdangerous bpathogens
bafter ba bmicrobiology blab, band byou bmay bspread bthem bout binto bthe bhallway
B)
Because byou bmay bpick bup bharmful bmicrobes bfrom bthe bhallway band bbring bthem
binto bthe blab bon byour blab bcoat
C)
Because bthe bother bpeople bin bthe bhallway bdon't bknow bwhether bor bnot byour blab
bcoat bis bcontaminated bwith bdangerous bmicroorganisms
D)
None bof bthe babove. bYou bshouldn't bwear byour blab bcoat bin bthe bhallway bafter ba
bmicrobiology blab. bBut bif byour blab bcoat bis bclean, bit's bfine bto bwear bit bin bthe
bhallway bbefore bthe blab bbegins b- b bcorrect banswer- bC)
Because bthe bother bpeople bin bthe bhallway bdon't bknow bwhether bor bnot byour blab
bcoat bis bcontaminated bwith bdangerous bmicroorganisms
According bto bthe bstandard boperating bprocedures bin byour blab bmanual, bwhat
bspecial bprecaution bshould bbe btaken bwhen bworking bwith blevel b2 bbacterial
bcultures?
A)
You bmust bwear bdisposable bgloves.
B)
You bmust bwear ba brespirator band bsafety bgoggles.
C)
,You bmust bwear ba bfull bbiosafety bcontainment bsuit. b(Like bthe bkind bthey bwear bin
bzombie bmovies)
D)
You bshould bnot bwork bwith blevel b2 bcultures. bLevel b2 bcultures bpresent ba bserious
brisk bto bhuman bhealth band bshould bonly bbe bhandled bby btrained bprofessionals. b- b
bcorrect banswer- bA)
You bmust bwear bdisposable bgloves.
True bor bFalse: bThe bmicrobiology blab bis bregistered bas ba bBSL-2 blab bbecause ball
bbacteria bare bconsidered bto bbe brisk bgroup b2 bmicroorganisms. b- b bcorrect
banswer- bFalse
Because bwe bwork bwith blive bcultures bin bthe bmicrobiology blab, bit bis bvery
bimportant bthat ball bof bour bused bmaterials bbe bdiscarded bin bthe bcorrect bway.
bMatch beach bof bthe bfollowing bused bitems bwith bthe bproper bdiscard blocation.
Answers bmay bbe bused bmore bthan bonce.
Paper btowels bused bto bdisinfect bthe bworkbench
Disposable bgloves
Used bPetri bplates
Swabs bused bto binoculate ba bPetri bplate
Used bglass bmicroscope bslides
1. bIn bthe blarge bbiohazard bbin bthat bsits bbeside bthe bdoor
2. bSharps/broken bglass bdiscard bcontainer
3. bIn bthe bregular bwaste bpaper bbaskets
4. bIt bdoesn't bmatter bwhere byou bdiscard bthis bitem, bbecause bif byou bput bit bin bthe
bwrong bplace, bsomeone bin bcharge bwill bjust bmove bit bto bthe bright blocation
banyway. b- b bcorrect banswer- b__3__ bPaper btowels bused bto bdisinfect bthe
bworkbench
__1__ bDisposable bgloves
__1__ bUsed bPetri bplates
__2__ bSwabs bused bto binoculate ba bPetri bplate
__2__ bUsed bglass bmicroscope bslides
Choose ball bof bthe bstatements bthat bare bTRUE. b(Note: bthis bis ba bmultiple bselect
bquestion. bYou bmust bchoose ball bof bthe btrue bstatements bto bget bthe bquestion
bcorrect.)
A)
Paper btowels bused bto bdisinfect bthe bworkbench bmust bbe bdisposed bof bin bthe
bbiohazard bbin
B)
Liquid bcultures bin bglass btest btubes bshould bbe bpoured bdown bthe bsink bwith
bplenty bof brunning bwater, band bthen bthe btest btubes bshould bbe bplaced bon bthe
bglassware btrolley
C)
, Gloves bmust balways bbe bdisposed bof bin bthe bbiohazard bbin b(even bif bthey bweren't
bused bto bhandle banything bbiohazardous)
D)
Swabs, btoothpicks, bdisposable bplastic bpipettes band banything belse beven bremotely
bpointy bshould balways bbe bdiscarded bin bthe bsharps/broken bglass bcontainer b- b
bcorrect banswer- bC)
Gloves bmust balways bbe bdisposed bof bin bthe bbiohazard bbin b(even bif bthey bweren't
bused bto bhandle banything bbiohazardous)
D)
Swabs, btoothpicks, bdisposable bplastic bpipettes band banything belse beven bremotely
bpointy bshould balways bbe bdiscarded bin bthe bsharps/broken bglass bcontainer
What bshould byou bdo bif byou baccidentally bspill ba bculture bin bthe bmicrobiology blab?
b(This bis ba bmultiple bselect bquestion, bchoose ball bthat bapply.)
A)
Stop, band bthink babout bwhat bthe bpotential bhazards bare band bhow bto bdeal bwith
bthem bin ba blogical border.
B)
Ask ba bTA bfor bhelp bcleaning bthe bspill bif bthere bis bbroken bglass binvolved.
C)
Move bany bleaking bcultures bto bthe bglassware bdiscard btrolley bimmediately.
D)
Wash byour bhands bif byou bspilled bculture bon bthem, bor bsuspect bthat byou bmight
bhave bspilled bculture bon bthem.
E)
Cover bthe bspill bwith bpaper btowel bbefore bsoaking bthe bpaper btowel bwith
bdisinfectant. b- b bcorrect banswer- bAll bare bcorrect
If ba bbacterial bculture bis bsplashed bon byour bskin, bwhat bis bthe bfirst bthing byou
bshould bdo?
Wash bskin bthoroughly bwith bsoap band bwater.
Inform bthe blab binstructor bor bTA.
Continue bworking bas bthe bculture bis brisk bgroup b1; btherefore bunlikely bto bcause
bdisease.
Wash bthe baffected barea bof bskin bwith ba bsmall bamount bof balcohol. b- b bcorrect
banswer- bWash bthe baffected barea bof bskin bwith ba bsmall bamount bof balcohol.
What bshould byou bdo bif byou baccidentally bget ba bbacterial bculture bin byour bmouth?
A)
Call b911 band btake ban bambulance bto bthe bnearest bhospital.
B)
Rinse byour bmouth bfor b30 bseconds bwith ban bantibacterial bmouthwash bsuch bas
bScope bor bListerine.
C)
Rinse byour bmouth bwith bcopious bamounts bof bwater band binform byour blab
binstructor.
D)
Do bnothing. bThe benzymes bin byour bmouth bwill beasily bdestroy bbacterial bcells. b- b
bcorrect banswer- bC)
WITH ALL QUESTIONS AND ALL
ANSWERS GIVEN
According bto bthe blab bstandard boperating bprocedures, byou bmust bwash byour
bhands bwith bsoap band bwater bfor bat bleast b_________ bbefore bleaving bthe blab.
A)
2 bseconds
B)
5 bseconds
C)
15 bseconds
D)
30 bseconds
E)
1 bminute b- b bcorrect banswer- bC) b
15 bseconds
Which bof bthe bfollowing bstatements bdoes bNOT bcorrectly bdescribe ba brule bthat
byou bmust bfollow bin bthe bmicrobiology blab?
A)
There bis bno beating bor bdrinking ballowed bin bthe bmicrobiology blab.
B)
If byou bspill ba bbacterial bculture bon byour blab bcoat, byou bmust breport bit bto byour
bdemonstrator.
C)
You bmust bdisinfect byour bworkbench bbefore band bafter beach bmicrobiology blab.
D)
No bpersonal beffects bare ballowed bin bthe blab. bYou bmust bfind bsomewhere belse bto
bleave byour bwinter bjacket, btextbooks, bbackpacks betc. bwhile byou battend bthe blab.
E)
,None bof bthe babove. b(All bof bthe babove bstatements bcorrectly bdescribe ba brule
bthat bmust bbe bfollowed bin bthe bmicrobiology blab) b- b bcorrect banswer- bE)
None bof bthe babove. b(All bof bthe babove bstatements bcorrectly bdescribe ba brule
bthat bmust bbe bfollowed bin bthe bmicrobiology blab)
Which bof bthe bfollowing bstatements bcorrectly bdescribe bthe blab bcoat bpolicy bin
bplace bin bthe bmicrobiology blab?
A)
You bmust bwear ba bbuttoned blab bcoat bat ball btimes bwhen bworking bin bthe
bmicrobiology blab.
B)
Between blabs byour blab bcoat bshould bbe bwashed bseparately bfrom bother bclothes
bwith bdetergent band bbleach.
C)
When byou btake byour blab bcoat bhome bto bwash bit byou bshould bcarry bit bin ba
bplastic bbag bto bkeep bit bseparate bfrom byour bother bbelongings.
D)
Your blab bcoat bcannot bbe bworn bin bany bother blab buntil bit bhas bbeen bwashed.
E)
All bof bthe babove bstatements bare btrue. b- b bcorrect banswer- bE)
All bof bthe babove bstatements bare btrue.
Which bof bthe bfollowing bstatements bbest bexplains bwhy byou bshould bnever bwear
byour bmicrobiology blab bcoat bin bthe bhallways, beither bbefore bor bafter ba blab?
b(Even bif bit's bclean.)
A)
Because byour blab bcoat bis blikely bto bbe bcontaminated bwith bdangerous bpathogens
bafter ba bmicrobiology blab, band byou bmay bspread bthem bout binto bthe bhallway
B)
Because byou bmay bpick bup bharmful bmicrobes bfrom bthe bhallway band bbring bthem
binto bthe blab bon byour blab bcoat
C)
Because bthe bother bpeople bin bthe bhallway bdon't bknow bwhether bor bnot byour blab
bcoat bis bcontaminated bwith bdangerous bmicroorganisms
D)
None bof bthe babove. bYou bshouldn't bwear byour blab bcoat bin bthe bhallway bafter ba
bmicrobiology blab. bBut bif byour blab bcoat bis bclean, bit's bfine bto bwear bit bin bthe
bhallway bbefore bthe blab bbegins b- b bcorrect banswer- bC)
Because bthe bother bpeople bin bthe bhallway bdon't bknow bwhether bor bnot byour blab
bcoat bis bcontaminated bwith bdangerous bmicroorganisms
According bto bthe bstandard boperating bprocedures bin byour blab bmanual, bwhat
bspecial bprecaution bshould bbe btaken bwhen bworking bwith blevel b2 bbacterial
bcultures?
A)
You bmust bwear bdisposable bgloves.
B)
You bmust bwear ba brespirator band bsafety bgoggles.
C)
,You bmust bwear ba bfull bbiosafety bcontainment bsuit. b(Like bthe bkind bthey bwear bin
bzombie bmovies)
D)
You bshould bnot bwork bwith blevel b2 bcultures. bLevel b2 bcultures bpresent ba bserious
brisk bto bhuman bhealth band bshould bonly bbe bhandled bby btrained bprofessionals. b- b
bcorrect banswer- bA)
You bmust bwear bdisposable bgloves.
True bor bFalse: bThe bmicrobiology blab bis bregistered bas ba bBSL-2 blab bbecause ball
bbacteria bare bconsidered bto bbe brisk bgroup b2 bmicroorganisms. b- b bcorrect
banswer- bFalse
Because bwe bwork bwith blive bcultures bin bthe bmicrobiology blab, bit bis bvery
bimportant bthat ball bof bour bused bmaterials bbe bdiscarded bin bthe bcorrect bway.
bMatch beach bof bthe bfollowing bused bitems bwith bthe bproper bdiscard blocation.
Answers bmay bbe bused bmore bthan bonce.
Paper btowels bused bto bdisinfect bthe bworkbench
Disposable bgloves
Used bPetri bplates
Swabs bused bto binoculate ba bPetri bplate
Used bglass bmicroscope bslides
1. bIn bthe blarge bbiohazard bbin bthat bsits bbeside bthe bdoor
2. bSharps/broken bglass bdiscard bcontainer
3. bIn bthe bregular bwaste bpaper bbaskets
4. bIt bdoesn't bmatter bwhere byou bdiscard bthis bitem, bbecause bif byou bput bit bin bthe
bwrong bplace, bsomeone bin bcharge bwill bjust bmove bit bto bthe bright blocation
banyway. b- b bcorrect banswer- b__3__ bPaper btowels bused bto bdisinfect bthe
bworkbench
__1__ bDisposable bgloves
__1__ bUsed bPetri bplates
__2__ bSwabs bused bto binoculate ba bPetri bplate
__2__ bUsed bglass bmicroscope bslides
Choose ball bof bthe bstatements bthat bare bTRUE. b(Note: bthis bis ba bmultiple bselect
bquestion. bYou bmust bchoose ball bof bthe btrue bstatements bto bget bthe bquestion
bcorrect.)
A)
Paper btowels bused bto bdisinfect bthe bworkbench bmust bbe bdisposed bof bin bthe
bbiohazard bbin
B)
Liquid bcultures bin bglass btest btubes bshould bbe bpoured bdown bthe bsink bwith
bplenty bof brunning bwater, band bthen bthe btest btubes bshould bbe bplaced bon bthe
bglassware btrolley
C)
, Gloves bmust balways bbe bdisposed bof bin bthe bbiohazard bbin b(even bif bthey bweren't
bused bto bhandle banything bbiohazardous)
D)
Swabs, btoothpicks, bdisposable bplastic bpipettes band banything belse beven bremotely
bpointy bshould balways bbe bdiscarded bin bthe bsharps/broken bglass bcontainer b- b
bcorrect banswer- bC)
Gloves bmust balways bbe bdisposed bof bin bthe bbiohazard bbin b(even bif bthey bweren't
bused bto bhandle banything bbiohazardous)
D)
Swabs, btoothpicks, bdisposable bplastic bpipettes band banything belse beven bremotely
bpointy bshould balways bbe bdiscarded bin bthe bsharps/broken bglass bcontainer
What bshould byou bdo bif byou baccidentally bspill ba bculture bin bthe bmicrobiology blab?
b(This bis ba bmultiple bselect bquestion, bchoose ball bthat bapply.)
A)
Stop, band bthink babout bwhat bthe bpotential bhazards bare band bhow bto bdeal bwith
bthem bin ba blogical border.
B)
Ask ba bTA bfor bhelp bcleaning bthe bspill bif bthere bis bbroken bglass binvolved.
C)
Move bany bleaking bcultures bto bthe bglassware bdiscard btrolley bimmediately.
D)
Wash byour bhands bif byou bspilled bculture bon bthem, bor bsuspect bthat byou bmight
bhave bspilled bculture bon bthem.
E)
Cover bthe bspill bwith bpaper btowel bbefore bsoaking bthe bpaper btowel bwith
bdisinfectant. b- b bcorrect banswer- bAll bare bcorrect
If ba bbacterial bculture bis bsplashed bon byour bskin, bwhat bis bthe bfirst bthing byou
bshould bdo?
Wash bskin bthoroughly bwith bsoap band bwater.
Inform bthe blab binstructor bor bTA.
Continue bworking bas bthe bculture bis brisk bgroup b1; btherefore bunlikely bto bcause
bdisease.
Wash bthe baffected barea bof bskin bwith ba bsmall bamount bof balcohol. b- b bcorrect
banswer- bWash bthe baffected barea bof bskin bwith ba bsmall bamount bof balcohol.
What bshould byou bdo bif byou baccidentally bget ba bbacterial bculture bin byour bmouth?
A)
Call b911 band btake ban bambulance bto bthe bnearest bhospital.
B)
Rinse byour bmouth bfor b30 bseconds bwith ban bantibacterial bmouthwash bsuch bas
bScope bor bListerine.
C)
Rinse byour bmouth bwith bcopious bamounts bof bwater band binform byour blab
binstructor.
D)
Do bnothing. bThe benzymes bin byour bmouth bwill beasily bdestroy bbacterial bcells. b- b
bcorrect banswer- bC)
