Exam (elaborations) OSMT

what are the 4 key things to making a blood smear️️-quality of smear -drying of the slide -fixation method -staining technique what do we use to fix blood smears️️methanol- preserve and look life like what are the factors that effect a good wedge smear (3)️️1) speed 2) to low- poor distribution 3) angle of the spreader what angle should the spreader be at️️30 degrees what angle do you need to make the slide thicker️️more then 30 what angle do you need to make the slide thinner️️less then 30 why do we do a retic count️️good way to see how the BM is responding what are reticulocytes️️immature RBC with fragments of DNA what stains do we use so we can see the fragments️️wright super vital incubate with new methylene blue or bromocresyl blue which microscope lense do you use to see a retic count️️100 x how many cells in total do you count for a retic️️1000 what should the field of view width be when doing a retic count️️200 what is the steps for preparing a retic count slide️️add blood to the stain, mix well, blood smear , let it dry and then look under microscope why is a absolute count more accurate️️it takes in the patients actual RBC in account what is the formula for absolute️️# of retics x RBC count what is the RR for absolute️️50-100 x 10 ^9 /l what does the percent retic assume️️it assumes the patient has a normal RBC count what is the formula for percent retic️️# retics /1000 rbc x100 how many granules do retics have️️2 or more if the patients retic is high what does that mean️️means there is bleeding inside , BM is making to much what are some disorders for high retic count️️hemolytic anemia after treatment for those with IDA hemmorhage (acute or chronic) is the patient has a low retic count , what does that mean