MIP 300 Final Exam Questions and
Answers
What is the minimum number of glucose molecules a bacterium that performs anaerobic
respiration would need to metabolize in order to get 380 ATP - Answer-11
Facultative anaerobes can do either aerobic respiration or fermentation. In an anaerobic
environment, which of the following could be an electron acceptor used by such a
facultative anaerobe? - Answer-pyruvate
When you follow a single Glyceraldehyde 3-P molecule forward toward the TCA cycle,
how many NADH are made when the number of carbons in this molecule has reached
two - Answer-2
why does Eukaryotic DNA takes much longer to replicate than Prokaryotic DNA -
Answer-Eukaryotes have to unwrap their DNA from histones during replication
During the carbon cycle some organisms produce CO2 and others fix CO2 which of the
following is/are true about these reactions that will produce CO2. - Answer-it occurs
when fats are oxidized during anerobic respiration.
During the carbon cycle some organisms produce CO2 and others fix CO2 which of the
following is/are true about the products produced and used to fix CO2. - Answer-aerobic
chemolithotrophs, anaerobic chemolithotrophs and photosynthetic organisms will
produce ATP and use it to provide the energy to create covalent bonds during CO2
fixation.
aerobic and anaerobic chemolithotrophic organism will produce NADH that will donate
electrons to CO2 reducing it.
Ligase will form a phosphodiester bond between the first nucleotide removed from the
RNA primer and the last DNA nucleotide on the previous Okazaki fragment - Answer-
false
The RNA primer is needed to - Answer-provide a 3' OH used to create a phosphodiester
bond to the first nucleotide added by DNA pol III.
What is the purpose of cyclohexamide? - Answer-it inhibits the growth of yeast
Which of the following can be mass produced through recombinant DNA technology?
a. DNA b. RNA c. Protein - Answer-dna, rna, protein
,During Polymerase Chain Reaction, which of the following steps can be performed at
the most variable temperatures? - Answer-annealing primers to dna
Interrupting the conjugation of two bacteria to map a chromosome is carried out using
________ as the donor - Answer-high frequency recombinant bacteria
Virulent/lytic phage are known NOT to kill a cell if: - Answer-Degraded cellular DNA is
packaged into the virion
For which type of transduction is it likely for genes very far from the prophage
integration site to be transferred - Answer-generalized transduction
What is the most likely event of forgetting the second wash of an anti HIV ELISA test? -
Answer-Goat anti-human antibody antibodies will not be washed away so the plate will
be fluorescent.
who provides the lipids in a viral envelope - Answer-the cell
all viruses have a protein coat - Answer-true
a virulent phage can inadvertently package host cell chromosome into phage particles -
Answer-true
you have a bacterium that picks up a new gene by Hfr conjugation. what is an
explanation of why progeny of this bacterium express this trait? - Answer-the gene is
integrated into the genome
after successful transformation of a fragment of chromosomal DNA, the recipient
(female) will become a donor (male) capable of creating a sex pili to transfer DNA -
Answer-false
you isolate a virulent page. It can perform specialized transduction - Answer-false
you want to know if a baby still has vaccine neutralizing antibody that it received from its
mom in utero. Which of the following types of ELISAs would you run? one that tests for -
Answer-IgG
in differential centrifugation, virus is first centrifuged at a slow speed, the supernatant is
removed and re-centrifuged at a very fast speed - Answer-true
Virus stocks prepared by differential centrifugation and gradient centrifugation are
equally clean - Answer-false
hemagglutination assay - Answer-determines highest dilution of virus that causes red
blood cells to clump together
, cytopathic effect - Answer-a visible effect on a host cell, caused by a virus, that may
result in host cell damage or death
immuno fluorescence assay - Answer-primarily on biological samples and is classically
defined as a procedure to detect antigens in cellular contexts using antibodies.
plaque assay - Answer-method used to measure the number of viral particles present in
a sample
a temperate phage can inadvertently package host cell chromosome into phage
particles - Answer-true
you isolate a bacterium that can transfer genes to other bacteria only when it is in direct
contact with these bacteria. it is most likely transferring the genes by - Answer-
conjugation
a piece of host cell dna bound to a piece of phage dna after excising from the
chromosome. when this phage that contains host cell dna infects a new cell, will the
new cell be killed by this infection? - Answer-no
after successful generalized transduction, the recipient (female) will become a donor
(male) capable of creating a sex pili to transfer dna - Answer-false
can a temperate phage perform generalized and specialized transduction? - Answer-
yes
what kind of organism can carry out the conversion of O2 --> H2O? - Answer-aerobic
respiration and aerobic chemolithotrophy
an error in dna replication causes a mutation changing AGG to CGG, what change do
you expect? - Answer-no change to protein function
the organism with the more positive reduction potential will produce more energy -
Answer-true
dna ligase forms - Answer-the last phosphodiester bond between dna single strands
dna ligase forms hydrogen bonds - Answer-false
for each acetyl-coA that enters the TCA cycle, how many ATP are generayed by
substrate level phosphorylation - Answer-1
you correctly perform a gran stain on bacteria that contain LPS, what color will you see
on the slide? - Answer-pink
Answers
What is the minimum number of glucose molecules a bacterium that performs anaerobic
respiration would need to metabolize in order to get 380 ATP - Answer-11
Facultative anaerobes can do either aerobic respiration or fermentation. In an anaerobic
environment, which of the following could be an electron acceptor used by such a
facultative anaerobe? - Answer-pyruvate
When you follow a single Glyceraldehyde 3-P molecule forward toward the TCA cycle,
how many NADH are made when the number of carbons in this molecule has reached
two - Answer-2
why does Eukaryotic DNA takes much longer to replicate than Prokaryotic DNA -
Answer-Eukaryotes have to unwrap their DNA from histones during replication
During the carbon cycle some organisms produce CO2 and others fix CO2 which of the
following is/are true about these reactions that will produce CO2. - Answer-it occurs
when fats are oxidized during anerobic respiration.
During the carbon cycle some organisms produce CO2 and others fix CO2 which of the
following is/are true about the products produced and used to fix CO2. - Answer-aerobic
chemolithotrophs, anaerobic chemolithotrophs and photosynthetic organisms will
produce ATP and use it to provide the energy to create covalent bonds during CO2
fixation.
aerobic and anaerobic chemolithotrophic organism will produce NADH that will donate
electrons to CO2 reducing it.
Ligase will form a phosphodiester bond between the first nucleotide removed from the
RNA primer and the last DNA nucleotide on the previous Okazaki fragment - Answer-
false
The RNA primer is needed to - Answer-provide a 3' OH used to create a phosphodiester
bond to the first nucleotide added by DNA pol III.
What is the purpose of cyclohexamide? - Answer-it inhibits the growth of yeast
Which of the following can be mass produced through recombinant DNA technology?
a. DNA b. RNA c. Protein - Answer-dna, rna, protein
,During Polymerase Chain Reaction, which of the following steps can be performed at
the most variable temperatures? - Answer-annealing primers to dna
Interrupting the conjugation of two bacteria to map a chromosome is carried out using
________ as the donor - Answer-high frequency recombinant bacteria
Virulent/lytic phage are known NOT to kill a cell if: - Answer-Degraded cellular DNA is
packaged into the virion
For which type of transduction is it likely for genes very far from the prophage
integration site to be transferred - Answer-generalized transduction
What is the most likely event of forgetting the second wash of an anti HIV ELISA test? -
Answer-Goat anti-human antibody antibodies will not be washed away so the plate will
be fluorescent.
who provides the lipids in a viral envelope - Answer-the cell
all viruses have a protein coat - Answer-true
a virulent phage can inadvertently package host cell chromosome into phage particles -
Answer-true
you have a bacterium that picks up a new gene by Hfr conjugation. what is an
explanation of why progeny of this bacterium express this trait? - Answer-the gene is
integrated into the genome
after successful transformation of a fragment of chromosomal DNA, the recipient
(female) will become a donor (male) capable of creating a sex pili to transfer DNA -
Answer-false
you isolate a virulent page. It can perform specialized transduction - Answer-false
you want to know if a baby still has vaccine neutralizing antibody that it received from its
mom in utero. Which of the following types of ELISAs would you run? one that tests for -
Answer-IgG
in differential centrifugation, virus is first centrifuged at a slow speed, the supernatant is
removed and re-centrifuged at a very fast speed - Answer-true
Virus stocks prepared by differential centrifugation and gradient centrifugation are
equally clean - Answer-false
hemagglutination assay - Answer-determines highest dilution of virus that causes red
blood cells to clump together
, cytopathic effect - Answer-a visible effect on a host cell, caused by a virus, that may
result in host cell damage or death
immuno fluorescence assay - Answer-primarily on biological samples and is classically
defined as a procedure to detect antigens in cellular contexts using antibodies.
plaque assay - Answer-method used to measure the number of viral particles present in
a sample
a temperate phage can inadvertently package host cell chromosome into phage
particles - Answer-true
you isolate a bacterium that can transfer genes to other bacteria only when it is in direct
contact with these bacteria. it is most likely transferring the genes by - Answer-
conjugation
a piece of host cell dna bound to a piece of phage dna after excising from the
chromosome. when this phage that contains host cell dna infects a new cell, will the
new cell be killed by this infection? - Answer-no
after successful generalized transduction, the recipient (female) will become a donor
(male) capable of creating a sex pili to transfer dna - Answer-false
can a temperate phage perform generalized and specialized transduction? - Answer-
yes
what kind of organism can carry out the conversion of O2 --> H2O? - Answer-aerobic
respiration and aerobic chemolithotrophy
an error in dna replication causes a mutation changing AGG to CGG, what change do
you expect? - Answer-no change to protein function
the organism with the more positive reduction potential will produce more energy -
Answer-true
dna ligase forms - Answer-the last phosphodiester bond between dna single strands
dna ligase forms hydrogen bonds - Answer-false
for each acetyl-coA that enters the TCA cycle, how many ATP are generayed by
substrate level phosphorylation - Answer-1
you correctly perform a gran stain on bacteria that contain LPS, what color will you see
on the slide? - Answer-pink
