Immunology BOC Midterm Exam
Questions And Verified Complete
Solutions.
Antinuclear antibody tests are performed to help diagnose - Answer lupus erythematosus
In the anti-double-stranded DNA procedure, the antigen most commonly utilized is - Answer Crithida
lucilia
Sera to be tested for IFA-ANA 6 days after drawing is best stored at - Answer 5 +/- 2
Antibodies directed at native DNA are most frequently associated with which pattern of fluorescence in
the IFA-ANA test - Answer rim
The technologist observes apparent homogenous staining of the nucleus of interphase cells while
performing an IFA-ANA, as well as staining of the chromosomes in mitotic cells. This result is - Answer
expected for anti-DNA antibodies
The result of anti-nuclear antibody test was a titer of 1:320 with a peripheral pattern. Which of the
following sets of results best correlate with these results - Answer anti-dsDNA titer 1:80, and a high
titer of antibodies to Sm
Systemic lupus erthematosus patients often have which of the following test results - Answer high
titers of DNA antibody
Systemic lupus erythematosus patients with active disease often have which of the following test results
- Answer marked decrease in serum CH50
Which of the following is decreased in serum during the active stages of systemic lupus erythematosus -
Answer complement (C3)
,A positive ANA with the pattern of anticentromere antibodies is most frequently seen in patients with -
Answer CREST syndrome
In the indirect fluorescent anti-nuclear antibody test, a homogenous pattern indicates the presence of
antibody to - Answer DNA
In the indirect fluorescent anti-nuclear antibody test, a speckled pattern may indicate the presence of
antibody to - Answer Sm
A patient has the following test results:
ANA + (1:320), ASO 50 Todd units, Complement decreased, RA +
The above results could be seen in patients with - Answer lupus erythematosus
Autoantibodies in the absence of Sm are found in patients with - Answer mixed connective tissue
disease
Which of the following is the most sensitive and appropriate method for the detection of rheumatoid
factor - Answer nephelometry
Rheumatoid factor reacts with - Answer gamma globulin-coated particles
A consistently and repeatedly negative IFA-ANA is - Answer strong evidence against untreated SLE
Positive rheumatoid factor is generally associated with - Answer hyperglobulinemia
The following results are from a rubella titer performed on acute and convalescent sera using a 2-fold
serial dilution
Date tested 1/23/04, Acute serum titer 1:8, Convalescent serum titer 1:32
After evaluating the above results, the best interpretation is - Answer results are consistent with active
infection with rubella
, Rheumatoid factors are immunoglobulins with specificity for allotypic determinants located on the -
Answer Fc fragment of IgG
Rheumatoid factor in patient's serum may cause a false - Answer positive test for the detection of IgM
class antibodies
Rheumatoid factors are defined as - Answer autoantibodies with specificity for the Fc portion of the
immunoglobulin (IgG) molecule
Tissue injury in systemic rheumatoid disorders such as SLE is thought to be caused by - Answer
deposition of immune complexes
False-positive rheumatoid factor in agglutination and nephelometric methods can be due to elevated
levels of - Answer C1q
An acute phase protein that binds to the membrane of certain microorganisms and activates the
complement system is - Answer C-reactive protein
High titers of antimicrosomal antibodies are most often found in - Answer thyroid disease
Which of the following is an organ-specific autoimmune disease - Answer Addison disease
In chronic active hepatitis, high titers of which of the following antibodies are seen - Answer anti-
smooth muscle
In primary biliary cirrhosis, which of the following antibodies are seen in high titers - Answer
antimitochondrial
Anti-RNA antibodies are often present in individuals having an anti-nuclear antibody immunofluorescent
pattern that is - Answer nucleolar
Questions And Verified Complete
Solutions.
Antinuclear antibody tests are performed to help diagnose - Answer lupus erythematosus
In the anti-double-stranded DNA procedure, the antigen most commonly utilized is - Answer Crithida
lucilia
Sera to be tested for IFA-ANA 6 days after drawing is best stored at - Answer 5 +/- 2
Antibodies directed at native DNA are most frequently associated with which pattern of fluorescence in
the IFA-ANA test - Answer rim
The technologist observes apparent homogenous staining of the nucleus of interphase cells while
performing an IFA-ANA, as well as staining of the chromosomes in mitotic cells. This result is - Answer
expected for anti-DNA antibodies
The result of anti-nuclear antibody test was a titer of 1:320 with a peripheral pattern. Which of the
following sets of results best correlate with these results - Answer anti-dsDNA titer 1:80, and a high
titer of antibodies to Sm
Systemic lupus erthematosus patients often have which of the following test results - Answer high
titers of DNA antibody
Systemic lupus erythematosus patients with active disease often have which of the following test results
- Answer marked decrease in serum CH50
Which of the following is decreased in serum during the active stages of systemic lupus erythematosus -
Answer complement (C3)
,A positive ANA with the pattern of anticentromere antibodies is most frequently seen in patients with -
Answer CREST syndrome
In the indirect fluorescent anti-nuclear antibody test, a homogenous pattern indicates the presence of
antibody to - Answer DNA
In the indirect fluorescent anti-nuclear antibody test, a speckled pattern may indicate the presence of
antibody to - Answer Sm
A patient has the following test results:
ANA + (1:320), ASO 50 Todd units, Complement decreased, RA +
The above results could be seen in patients with - Answer lupus erythematosus
Autoantibodies in the absence of Sm are found in patients with - Answer mixed connective tissue
disease
Which of the following is the most sensitive and appropriate method for the detection of rheumatoid
factor - Answer nephelometry
Rheumatoid factor reacts with - Answer gamma globulin-coated particles
A consistently and repeatedly negative IFA-ANA is - Answer strong evidence against untreated SLE
Positive rheumatoid factor is generally associated with - Answer hyperglobulinemia
The following results are from a rubella titer performed on acute and convalescent sera using a 2-fold
serial dilution
Date tested 1/23/04, Acute serum titer 1:8, Convalescent serum titer 1:32
After evaluating the above results, the best interpretation is - Answer results are consistent with active
infection with rubella
, Rheumatoid factors are immunoglobulins with specificity for allotypic determinants located on the -
Answer Fc fragment of IgG
Rheumatoid factor in patient's serum may cause a false - Answer positive test for the detection of IgM
class antibodies
Rheumatoid factors are defined as - Answer autoantibodies with specificity for the Fc portion of the
immunoglobulin (IgG) molecule
Tissue injury in systemic rheumatoid disorders such as SLE is thought to be caused by - Answer
deposition of immune complexes
False-positive rheumatoid factor in agglutination and nephelometric methods can be due to elevated
levels of - Answer C1q
An acute phase protein that binds to the membrane of certain microorganisms and activates the
complement system is - Answer C-reactive protein
High titers of antimicrosomal antibodies are most often found in - Answer thyroid disease
Which of the following is an organ-specific autoimmune disease - Answer Addison disease
In chronic active hepatitis, high titers of which of the following antibodies are seen - Answer anti-
smooth muscle
In primary biliary cirrhosis, which of the following antibodies are seen in high titers - Answer
antimitochondrial
Anti-RNA antibodies are often present in individuals having an anti-nuclear antibody immunofluorescent
pattern that is - Answer nucleolar
