Tools for the Laboratory
Exam Questions and
Complete Solutions
Graded A+
Denning [Date] [Course title]
, 5 I's for culturing microbes - Answer: inoculation
incubation
isolation
inspection
identification
inoculation - Answer: place a sample on the media
incubation - Answer: typically in an incubator set at 37 degrees or 25 degrees
isolation - Answer: observe colonies (pour plate, streak plate)
inspection - Answer: appearance, results of biochemical tests
identification - Answer: summation of all I's
solid - Answer: agar
liquid - Answer: broth
3 properties media is classified by - Answer: physical state
chemical composition
functional type or purpose
general purpose media - Answer: grown a broad variety of organisms (nutrient agar)
enriched media - Answer: contains things like blood or special growth factors to allow certain species to
grow