Gel Electrophoresis – Questions With Correct Answers

Gel Electrophoresis – Questions With Correct Answers

Aa, Aa, Aa, Aa Right Ans - 100% heterozygos

AA, Aa, Aa, aa Right Ans - 3:1 ratio

Gel electrophoresis Right Ans - technique used to separate DNA fragments
according to their size and charge ***

Based on their size and charge, the molecules will travel through the gel
Right Ans - at different speeds, allowing them to be separated from one
another

DNA samples are loaded into wells (indentations) Right Ans - at one end of
a gel, and an electric current is applied to pull them through the gel

DNA fragments are negatively charged, Right Ans - so they move towards
the positive electrode

DNA is ___ charged* Right Ans - negatively*

Because all DNA fragments have the same amount of charge per mass,
Right Ans - small fragments move through the gel faster than large ones

When a gel is stained with a DNA-binding dye, Right Ans - the DNA
fragments can be seen as bands, each representing a group of same-sized DNA
fragments

Using electrophoresis, Right Ans - we can see how many different DNA
fragments are present in a sample and how large they are relative to one
another

We can also determine the absolute size of a piece Right Ans - of DNA by
examining it next to a standard "yardstick" made up of DNA fragments of
known sizes. (DNA Ladder)