Electrophoresis Lab – Questions & Accurate Answers
a machine used to separate nucleic acids and proteins is a _______ Right Ans
- gel electrophoresis
Has an electric current to move molecules based on mlolecular weight and
causes positive charges to move to positve pole of box, and negative charges
to move to negative pole of box is a ___________. Right Ans - gel
electrophoresis
Causes smaller molecules to appear to travel further down gel but they are
really just moving faster is a Right Ans - gel electrophoresis
A gel used to seperate nucliec acids and large protiens, is composed of sea
weed is _________. Right Ans - agarose gel
Agarose gel is usually 0.7%-3% agarose but the most common is ______ agarose
gel Right Ans - 1.0%
Causes a setting result from physical process and the distance between bands
is determined by _________. Right Ans - the density of agarose
A gel used to seperate proteins and requires uniform pore size is __________.
Right Ans - polyacrylamide electrophoresis gel(PAGE)
A potent neurotoxin in powder and liquid form is ______. Right Ans -
acrylamide
A process where stacking gels are poured over resolving gels, stacking gel
contains less acrylomide (5% max), a lower pH, and a different ionic content,
the proteins are concentrated into a tight band in stacking gel before entering
resolving gel is _______. Right Ans - PAGE gel
A gel used to seperate nondenatured protiens by charge and size and is
visualized with black staining is a __________. Right Ans - starch gel
a machine used to separate nucleic acids and proteins is a _______ Right Ans
- gel electrophoresis
Has an electric current to move molecules based on mlolecular weight and
causes positive charges to move to positve pole of box, and negative charges
to move to negative pole of box is a ___________. Right Ans - gel
electrophoresis
Causes smaller molecules to appear to travel further down gel but they are
really just moving faster is a Right Ans - gel electrophoresis
A gel used to seperate nucliec acids and large protiens, is composed of sea
weed is _________. Right Ans - agarose gel
Agarose gel is usually 0.7%-3% agarose but the most common is ______ agarose
gel Right Ans - 1.0%
Causes a setting result from physical process and the distance between bands
is determined by _________. Right Ans - the density of agarose
A gel used to seperate proteins and requires uniform pore size is __________.
Right Ans - polyacrylamide electrophoresis gel(PAGE)
A potent neurotoxin in powder and liquid form is ______. Right Ans -
acrylamide
A process where stacking gels are poured over resolving gels, stacking gel
contains less acrylomide (5% max), a lower pH, and a different ionic content,
the proteins are concentrated into a tight band in stacking gel before entering
resolving gel is _______. Right Ans - PAGE gel
A gel used to seperate nondenatured protiens by charge and size and is
visualized with black staining is a __________. Right Ans - starch gel
