Biochemie
1
,Inhoud
Biochemie .................................................................................................................................. 1
inleiding .................................................................................................................................. 4
Eerste wet thermodynamica.............................................................................................. 4
Tweede wet thermodynamica ........................................................................................... 4
Eiwitsamenstelling en structuur ............................................................................................ 4
Proteïnen bestaan uit aminozuren .................................................................................... 4
Primaire structuren ............................................................................................................ 7
Secundaire structuren........................................................................................................ 8
Tertiaire structuren ............................................................................................................ 8
Quaternaire structuren ...................................................................................................... 9
Aminozuur sequentie ............................................................................................................. 9
Afbraak van RNA in oplossing ............................................................................................ 9
Eiwitten en proteomen ........................................................................................................ 10
Purificatie van proteïnen ................................................................................................. 10
Immunologie ........................................................................................................................ 13
Indirecte ELISA ................................................................................................................. 14
Sandwish ELISA ................................................................................................................ 14
Western-blotting.............................................................................................................. 14
Massaspectrometrie ........................................................................................................ 14
Edman degradatie ............................................................................................................ 15
MS-MS .............................................................................................................................. 15
Sequentie bepalen door overlapping .............................................................................. 16
Enzymes: algemene concepten en kinetica ......................................................................... 16
Enzymes als specifieke katalysatoren .............................................................................. 16
Michaëlis menten model ................................................................................................. 16
Catalytische strategiën......................................................................................................... 18
Proteasen ......................................................................................................................... 18
Carbonische anhydrase .................................................................................................... 20
Restrictie enzymes ........................................................................................................... 21
Regulatie van enzymen ........................................................................................................ 22
Aspartaat transcarbamoylase (ATCase) ........................................................................... 22
Isozymes ........................................................................................................................... 23
Cyclische AMP .................................................................................................................. 23
Enzyme activatie door proteolytische splitsing (zymogeen) ............................................... 24
2
, Chymotrypsinogeen ......................................................................................................... 24
Trypsinogeen.................................................................................................................... 24
Bloedklonters ................................................................................................................... 24
Koolhydraten............................................................................................................................ 25
Monosachariden .................................................................................................................. 25
Fructose ........................................................................................................................... 25
Glucose............................................................................................................................. 26
3
1
,Inhoud
Biochemie .................................................................................................................................. 1
inleiding .................................................................................................................................. 4
Eerste wet thermodynamica.............................................................................................. 4
Tweede wet thermodynamica ........................................................................................... 4
Eiwitsamenstelling en structuur ............................................................................................ 4
Proteïnen bestaan uit aminozuren .................................................................................... 4
Primaire structuren ............................................................................................................ 7
Secundaire structuren........................................................................................................ 8
Tertiaire structuren ............................................................................................................ 8
Quaternaire structuren ...................................................................................................... 9
Aminozuur sequentie ............................................................................................................. 9
Afbraak van RNA in oplossing ............................................................................................ 9
Eiwitten en proteomen ........................................................................................................ 10
Purificatie van proteïnen ................................................................................................. 10
Immunologie ........................................................................................................................ 13
Indirecte ELISA ................................................................................................................. 14
Sandwish ELISA ................................................................................................................ 14
Western-blotting.............................................................................................................. 14
Massaspectrometrie ........................................................................................................ 14
Edman degradatie ............................................................................................................ 15
MS-MS .............................................................................................................................. 15
Sequentie bepalen door overlapping .............................................................................. 16
Enzymes: algemene concepten en kinetica ......................................................................... 16
Enzymes als specifieke katalysatoren .............................................................................. 16
Michaëlis menten model ................................................................................................. 16
Catalytische strategiën......................................................................................................... 18
Proteasen ......................................................................................................................... 18
Carbonische anhydrase .................................................................................................... 20
Restrictie enzymes ........................................................................................................... 21
Regulatie van enzymen ........................................................................................................ 22
Aspartaat transcarbamoylase (ATCase) ........................................................................... 22
Isozymes ........................................................................................................................... 23
Cyclische AMP .................................................................................................................. 23
Enzyme activatie door proteolytische splitsing (zymogeen) ............................................... 24
2
, Chymotrypsinogeen ......................................................................................................... 24
Trypsinogeen.................................................................................................................... 24
Bloedklonters ................................................................................................................... 24
Koolhydraten............................................................................................................................ 25
Monosachariden .................................................................................................................. 25
Fructose ........................................................................................................................... 25
Glucose............................................................................................................................. 26
3
