QBM UCF Final Exam Assorted terms 2024

QBM UCF Final Exam Assorted terms 2024 Molecular Biology - answerThe study, analysis, and manipulation of DNA and proteins. Recombinant DNA Technology - answerThe process of taking a gene from one source and joining it with another piece of DNA and inserting that DNA into bacteria for further study and manipulation OSHA - answerOccupational Health and Safety Administration State that employers must inform employees about any potential work place hazard. MSDS - answerSheet used to describe a chemical's physical and chemical properties and any potential hazards it may present. CDC - answerCenter for Disease Control Regulates any research that involves disease-causing agents BSL 1 - answerEnvironment presents limited hazards to any researchers/employees BSL 2 - answerLabs that present researchers with moderate risks. Any employees must be trained and certified to work with equipment and any hazardous chemicals need to disposed of properly. Most labs on UCF campus are BSL 2 BSL 3 - answerLabs that work with pathogenic agents capable of causing disease. Require proper ventilation BSL 4 - answerLabs that work with dangerous pathogens that cause fatal diseases and for which there are no vaccines. PPE - answerPersonal Protective Equipment Gloves, Labcoat, Goggles Latex gloves are best Note: Never recap needles Graduated Cylinders - answerLarge volumes of 25 mL to 2 L Pasteur Pipetts - answerTransfer pipette used when specific volumes are not required. Micropipettes - answerMost accurate measuring device Centrifuge - answerSeparates solutions based on density. Require precise balancing. g = Relative Centrifugal Force (universal) Analytical Centifugation - answerSedimentation characteristics of pure samples can be analyzed. Used to determine purity,molecular weight, size and diffusion coefficients. Ultracentrifuge Large-capacity Centrifugation - answerUsed to separate large volumes (up to 2 L) of bacteria. capable of reaching 6500 g High-speed Centrifugation - answerUsed for volumes of about 50 mL. Separate microorganisms and cellular debris Fixed-angle Rotors - answerPerfect for spinning pellets Swinging-bucket Rotors - answerAnalytical Used for cesium chloride gradients. Used by Messelson-Stahl to analyze DNA replication (semi-conservative) Clear bottles - answerBetter visualization but are less resilient to cracking and chemicals Spectrometry - answerThe study of radiation interacting with matter Spectrophotometers - answerMeasure the intensity of the wave length of light as it passes through a sample solution. Measures absorbance Plate Readers - answerAdvanced spectrophotometers that can measure the absorbency of hundreds of samples at once Used in ELISAs, drug screenings, and enzyme assays. Spectroscopy - answerThe separation of light into its wave lengths 260 nm - answerDNA 280 nm - answerProteins 595 nm - answerBradford Assay 600 nm - answerBacterial growth in LB agar 750 nm - answerLowry Assay Absorbance - answerDirectly related to concentration. A = EBc = -log(T) (Beer's Law) Transmittance - answerT=10^(-A) Why does DNA absorb light? - answerDue to its ringed nature, DNA will absorb light in the UV spectrum Why do Proteins absorb light? - answerDue to the ringed amino acids, Tryptophan and Tyrosine Most accurate method of protein purification? - answerAmino Acid Analysis Unrealistic--expensive/long turnaround Bradford Assay - answerFast/Simple but inaccurate (high variability) Coomassie Brilliant Blue binds to proteins and turns blue Will absorb at 595 nm Typically used as a quick check step and requires a standard curve Lowry Method - answerCopper binds to the proteins and produces Cu+. The Folin reagent reacts with Cu+ and turns blue Will absorb at 750 nm Very time sensitive and requires a standard curve DC-Assay - answerA detergent-compatible lowry assay