MIP 300 - Exam 4 question with verified solutions 2023/2024

MIP 300 - Exam 4 You isolate a new temperate phage. It can - correct answer integrate into the genome reproduce to make new viral particles enter the lytic cyle Both virulent and temperate phase can carry out specialized transduction - correct answer False Both virulent and temperate phase can carry out generalized transduction - correct answer True A non capsule producing Streptococci pneumoniae bacterium takes up a linear chromosomal piece of exogenous DNA that contains the gene for producing a capsule. It does not integrate into the chromosome and is not contained on a plasmid. You grow up large amounts of this bacterium and inject it into a mouse. What do you expect will occur in this mouse? Group of answer choices - correct answer It will live A temperate phage can transfer genes to new bacteria that it obtains while excising from the host chromosome. - correct answer True A temperate phage can enter the lytic cycle. - correct answer True A virulent phage can inadvertently package host cell chromosome into phage particles. - correct answer True You isolate a bacterium that can transfer genes to other bacteria. You isolate the medium this bacterium is growing in (being very careful not to have any of the bacteria in the medium), and treat it with protease, then add new bacteria and allow them to grow in this medium. You find these bacteria have acquired new genes. The bacterium you isolated is most likely transferring genes by - correct answer Transformation You are studying the Griffith experiments or the experiments that followed. You take mice and inject them with dead strain R bacteria treated with protease and mixed with live strain S bacteria. Your lab partner predicts the mice will die. Your partner is correct - correct answer True You are studying a lysogenic phage. When a phage particle that contains phage DNA infects a new cell, will the new cell be killed by this infection? - correct answer Maybe You are studying a phage that packages a fragment of host cell DNA that is about the same size as the phage genome in the process of packaging. When this phage that contains host cell DNA infects a new cell, will the new cell be killed by this infection? - correct answer No You are studying the Griffith experiments or the experiments that followed. You take mice and inject them with dead strain S bacteria treated with DNAse and mixed with live strain R bacteria. Your lab partner predicts the mice will live. Your partner is correct - correct answer True You are studying the Griffith experiments or the experiments that followed. You take mice and inject them with dead strain S bacteria treated with protease and mixed with live strain R bacteria. Your lab partner predicts the mice will live. Your partner is correct - correct answer False You have a bacterium that picks up a new gene by transformaton. Which of the following is a likely explanation of why the progeny of this bacterium express this trait? - correct answer The gene is integrated into the genome You have a bacterium that picks up a new gene by specialized transduction. Which of the following is a likely explanation of why the progeny of this bacterium express this trait? - correct answer The gene is integrated into the genome After successful generalized transduction, the recipient (female) will become a donor (male) capable of creating a sex pili to transfer DNA - correct answer False After successful transformation of a fragment of chromosomal DNA, the recipient (female) will become a donor (male) capable of creating a sex pili to transfer DNA - correct answer False