Chemistry MT AAB board exam review #1 with 100% correct answers.

Specimens that can be analyzed for carbon dioxide 1. Serum 2. Plasma 3. Whole blood What will happen if blood is exposed to air during specimen collection for pH and blood gases? p C O 2 decreases Henderson-Hasselbalch equation p H = p k a + log salt / acid What is the ratio of bicarbonate:carbonic acid in normal plasma 20:1 Lab results on a known diabetic patient revealed a decreased bicarbonate and decreased ph. Which acid base disorder would most likely cause these lab values? Metabolic acidosis Decrease Bicarbonate decrease p H Normal range for pH of blood 7:35 to 7:45 Beer law concerning optical density it is: Directly proportional to the concentration For the ultraviolet range what part must be employed Quartz cuvette Quartz cuvette When performing spectrophotometer quality checks, what is the holmium oxide glass filter used to assess? Wavelength accuracy What is the principle of flame photometry? Emission of a color when an element is burned What is the principle of atomic absorption spectrophotometry? It measures light absorption of electromagnetic radiation What is the light source in atomic absorption instrumentation Cathode lamp Quenching is a disadvantage in which type of instrumentation Fluorometry Which analytical method measures the amount of light scattered by the particles in solution Nephelometry p c o 2 electrode The actual pH of the sample has no effect The protein at its isoelectric point Net zero charge In immunoelectrophoresis There must be excess antibody present and constant trough distance between antibody and antigen To achieve the best levels of sensitivity and specificity, a gas chromatograph may be coupled with a Mass spectrophotometer Which colligative property is most commonly used in osmometry Freezing point Zimmerman reaction is used to detect 17-ketosteroids Hormone appears to function solely in the initiation and maintenance of lactation? Prolactin Hormone acts on the renal tubules to increase water reabsorption Anti diuretic hormone What is the major binding protein for thyroxine Thyroid binding globulin What is T3 uptake measuring Thyroid binding globulin Which hormone is important in bone and calcium metabolism Calci tonin Which hormone stimulates the renal production of vitamin D PTH Cathecholamines are secreted by Adrenal medulla The metabolite of epinephrine Vanillymandelic acid HCG is secreted by The uterus When does the highest level of HCG occur First trimester Hormone synthesized from cholesterol Testosterone What is the urinary metabolite so serotonin 5-hydroxyindoleacetic acid Migration sequence from the origin, of lipoprotein during electrophoresis Chylomicrons / beta/ pre-beta alpha Reagents used for color development in the Liebermann-Burchard reaction for cholesterol Acetic anhydride/sulfuric acid The purpose of the saponification step in cholesterol methods Convert cholesterol esters to free cholesterol Most triglycerides procedures involve the measurement of which end product Glycerol Friedwald formula by which LDL cholesterol can be calculated LDL cholesterol = cholesterol - (triglycerides / 5 + HDL cholesterol) When calculating a coronary risk index for a patient, one must know the total cholesterol and which other parameter? HDL cholesterol What is the method of choice for confirming drugs detected by screening methods? Gas chromatography-mass spectrophotometry which condition is associated with a decreased CSF glucose level? Bacterial meningitis Oligoclonal banding during CSF electrophoresis is characteristically seen in which condition Multiple Sclerosis which plasma substances is Not normally filtered through the glomerulus in significant amounts Protein Systems may be used to determine the specific gravity of urine Refractometer / harmonic oscillation / reagent test strip What is the best single indicator of renal disease Proteinuria A glitter cell is a term used to describe Leukocyte The matrix of urinary casts has been identified as which protein Tamm Horsfall mucoprotein Which one of the following casts is seen in acute glomerulonephritis Red blood cells