Microbiology Lab Exam #1 Study Guide – With Questions & Correct Answers

Ocular/Eyepiece - ANSWER - Binocular microscopes have two. They magnify the image tenfold. Body - ANSWER - It contains mirrors that bend the light into the ocular. Nosepiece - ANSWER - Where the objectives are attached to. Objectives - ANSWER - Contains lenses that magnify the image. A microscope has 4x, 10x, 40x and 100x Arm - ANSWER - It supports the nosepiece, holds on/off toggle and focus adjustment knobs. Stage/Mechanical stage - ANSWER - The black, flat platform on which your slide rests. Its the silver apparatus that actually holds the slide and moves the slide to position it on the stage. Stage Adjustment knobs - ANSWER - These move the the mechanical stage Light Intensity Control - ANSWER - This knob increases or decreases light output from the light source. Substage condenser - ANSWER - It is made of at least two lenses that focus the light passing through the specimen and improve image sharpness. Aperture diaphragm control - ANSWER - Controls the amount of light entering the substage condenser from the light source. Field diaphragm lever - ANSWER - It opens and closes to increase and decrease light from the light source. It should remain open Light source - ANSWER - The illuminator is a halogen bulb that produces light. Base - ANSWER - It holds the illuminator and supports the rest of the microscope. Fine focus adjustment knob - ANSWER - Brings the image into sharp focus. This knob raises and lowers the stage in very small increments.