Name: Hiba Majid (P:\Science\Mr Alim\Year 12\AS Biology)
(Core Practical 11 )
Title: Investigating photosynthesis
METHOD
1. Carry out instructions given (or those you have produced and researched) to complete the
practical (CPAC1)
2. List apparatus below and describe techniques used when measuring quantities with a
particular piece of apparatus:
Piece of apparatus Purpose of piece of Comment on how piece of equipment Appendix
apparatus was used 5c
accurately/appropriately/safely technique
Spinach leaves Spinach is the plant This may also be contaminated as it is
specimen from which the in a lab environment. To avoid
chloroplasts will be sickness, hands are to be washed after
obtained so that these handling and the spinach leaves are
can be isolated and not to be consumed
observed
Low risk
Pestle and mortar To grind the spinach to Keep away from the table so that it
break the cells and doesn’t fall and break, the broken
release chloroplasts piece may cut someone’s skin. We will
handle it responsibly and not throw it
around to avoid breakages
Low risk
5 test tubes These will hold our The tubes will be held by a test tube
solutions of filtrate to be rack. The rack will be kept away from
placed in the different the edge of the table to avoid
conditions shattering of the tube
Low risk
Ice and salt To keep the filtrate cool Keep bucket away from the edge of
(slow down enzyme the table to avoid the water spilling
activity which would lead and becoming a hazard that someone
to damage of the might slip on and hurt themselves
chloroplasts)
Low risk
DCPIP Acts as replacement for The bottle of DCPIP will have its lid on
NADP as the electron when not in use be kept away from
acceptor, observe colour the edge of the table to avoid falling
change from blue to off and leading to spillages
Mr Alim
, Name: Hiba Majid (P:\Science\Mr Alim\Year 12\AS Biology)
colourless To avoid staining, we will wear gloves
and goggles
Low risk
Isolation medium This will stop the The bottle of isolation medium will
chloroplasts from have its lid on when not in use be kept
shrivelling up or bursting away from the edge of the table to
(due to osmosis). it also avoid falling off and leading to
slows down enzyme spillages
activity
Low risk
Glass rod To mix the isolation The glass rod will be kept far away from
medium and chloroplast the edge of the table so that it doesn’t
pellet when it is being roll off the table and smash. The
resuspended smashed pieces could potentially injure
someone who tries to pick it up or
steps on it accidentally.
Medium risk
Muslin To filter out the larger We will cut the muslin into moderate
pieces of the spinach sized squares to avoid wasting it.
No risk
Lamp To see the effect of light We will keep the lamp away from the
intensity on test tubes at the same distance away
photosynthesis which will be 12-15cm, for all our
results.
To avoid eye damage, we will not look
directly at the lamp bulb.
It is also an electrical piece of
equipment and so will be kept away
from any liquids
Low risk
Centrifuge and The centrifuge tube will We will keep the machine in the
centrifuge tube have filtrate containing middle of the table so that it does not
chlorophyll. This will then fall off and potentially break or fall on
be put in the centrifuge to someone's foot
gather the chloroplast to
low risk
form a pellet.
Pipette pump and To measure out the filtrate Ensure the glass part of the pipette is
glass pipette and isolation medium in the middle of the table so that it
when transferring it to the doesn’t roll off the table and smash.
beakers, centrifuge tubes The smashed pieces could potentially
and test tubes. injure someone who tries to pick it up
Mr Alim
(Core Practical 11 )
Title: Investigating photosynthesis
METHOD
1. Carry out instructions given (or those you have produced and researched) to complete the
practical (CPAC1)
2. List apparatus below and describe techniques used when measuring quantities with a
particular piece of apparatus:
Piece of apparatus Purpose of piece of Comment on how piece of equipment Appendix
apparatus was used 5c
accurately/appropriately/safely technique
Spinach leaves Spinach is the plant This may also be contaminated as it is
specimen from which the in a lab environment. To avoid
chloroplasts will be sickness, hands are to be washed after
obtained so that these handling and the spinach leaves are
can be isolated and not to be consumed
observed
Low risk
Pestle and mortar To grind the spinach to Keep away from the table so that it
break the cells and doesn’t fall and break, the broken
release chloroplasts piece may cut someone’s skin. We will
handle it responsibly and not throw it
around to avoid breakages
Low risk
5 test tubes These will hold our The tubes will be held by a test tube
solutions of filtrate to be rack. The rack will be kept away from
placed in the different the edge of the table to avoid
conditions shattering of the tube
Low risk
Ice and salt To keep the filtrate cool Keep bucket away from the edge of
(slow down enzyme the table to avoid the water spilling
activity which would lead and becoming a hazard that someone
to damage of the might slip on and hurt themselves
chloroplasts)
Low risk
DCPIP Acts as replacement for The bottle of DCPIP will have its lid on
NADP as the electron when not in use be kept away from
acceptor, observe colour the edge of the table to avoid falling
change from blue to off and leading to spillages
Mr Alim
, Name: Hiba Majid (P:\Science\Mr Alim\Year 12\AS Biology)
colourless To avoid staining, we will wear gloves
and goggles
Low risk
Isolation medium This will stop the The bottle of isolation medium will
chloroplasts from have its lid on when not in use be kept
shrivelling up or bursting away from the edge of the table to
(due to osmosis). it also avoid falling off and leading to
slows down enzyme spillages
activity
Low risk
Glass rod To mix the isolation The glass rod will be kept far away from
medium and chloroplast the edge of the table so that it doesn’t
pellet when it is being roll off the table and smash. The
resuspended smashed pieces could potentially injure
someone who tries to pick it up or
steps on it accidentally.
Medium risk
Muslin To filter out the larger We will cut the muslin into moderate
pieces of the spinach sized squares to avoid wasting it.
No risk
Lamp To see the effect of light We will keep the lamp away from the
intensity on test tubes at the same distance away
photosynthesis which will be 12-15cm, for all our
results.
To avoid eye damage, we will not look
directly at the lamp bulb.
It is also an electrical piece of
equipment and so will be kept away
from any liquids
Low risk
Centrifuge and The centrifuge tube will We will keep the machine in the
centrifuge tube have filtrate containing middle of the table so that it does not
chlorophyll. This will then fall off and potentially break or fall on
be put in the centrifuge to someone's foot
gather the chloroplast to
low risk
form a pellet.
Pipette pump and To measure out the filtrate Ensure the glass part of the pipette is
glass pipette and isolation medium in the middle of the table so that it
when transferring it to the doesn’t roll off the table and smash.
beakers, centrifuge tubes The smashed pieces could potentially
and test tubes. injure someone who tries to pick it up
Mr Alim
