CHEM 182 DL1
Chromat ography of Food Dyes
Final Report
St ude nt Moham m ed Alnaim i
Name
St ude nt ID 48408
Le s s o n C hrom atography of Food Dyes
Ins t it ut io n O cean C ounty C ollege
Se s s io n 2019L3 C HEM 182 DL1
Co ur s e C HEM 182 DL1
Ins t r uc t o r Nancy Marashi
Exercise 1
1. What might happe n if a s t ude nt us e d a pe n t o mar k t he bas e line o n t he
c hr o mat o gr aphy pape r ?
There will be a separation of com ponents of ink. This could create an error with the com ponents
that are trying to separate. This said, a pen could not be used.
2. What wo uld happe n if t he s t ude nt did no t s t o p t he de ve lo pme nt be f o r e t he s o lve nt
r e ac he d t he t o p o f t he pape r ?
The solution will m ove to the top and you would not be able to calculate the Rf value that is
com pared to the distance that a com ponent traveled in the stationary phase reached by the
solvent.
3. What wo uld happe n if t he s o lve nt le ve l was abo ve t hat o f t he dye s po t s o n t he pape r
at t he s t ar t o f t he de ve lo pme nt ?
The solvent would wash away the dye.
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, 4. Why is it impo r t ant t o mar k t he s o lve nt le ve l o n t he c hr o mat o gr aphy pape r as s o o n as
yo u r e mo ve it f r o m t he Pe t r i dis h?
Because the solvents used for chrom atography are volatile. If it is not m arked im m ediately,
there
is a possibility that it m ay evaporate and then you would not be able to draw the solvent.
Photo 1: Chromatogram
Data Table 1: Chromatogram Data
Sample Solve nt front (mm)
Blue 1 48
Blue 2 48
Re d 3 48
Re d 40 48
Ye llow 5 48
Ye llow 6 48
UK
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Chromat ography of Food Dyes
Final Report
St ude nt Moham m ed Alnaim i
Name
St ude nt ID 48408
Le s s o n C hrom atography of Food Dyes
Ins t it ut io n O cean C ounty C ollege
Se s s io n 2019L3 C HEM 182 DL1
Co ur s e C HEM 182 DL1
Ins t r uc t o r Nancy Marashi
Exercise 1
1. What might happe n if a s t ude nt us e d a pe n t o mar k t he bas e line o n t he
c hr o mat o gr aphy pape r ?
There will be a separation of com ponents of ink. This could create an error with the com ponents
that are trying to separate. This said, a pen could not be used.
2. What wo uld happe n if t he s t ude nt did no t s t o p t he de ve lo pme nt be f o r e t he s o lve nt
r e ac he d t he t o p o f t he pape r ?
The solution will m ove to the top and you would not be able to calculate the Rf value that is
com pared to the distance that a com ponent traveled in the stationary phase reached by the
solvent.
3. What wo uld happe n if t he s o lve nt le ve l was abo ve t hat o f t he dye s po t s o n t he pape r
at t he s t ar t o f t he de ve lo pme nt ?
The solvent would wash away the dye.
Copyrigh t 2019 - H an ds-On Labs | h t t p://h olscien ce.com 1/ 5
, 4. Why is it impo r t ant t o mar k t he s o lve nt le ve l o n t he c hr o mat o gr aphy pape r as s o o n as
yo u r e mo ve it f r o m t he Pe t r i dis h?
Because the solvents used for chrom atography are volatile. If it is not m arked im m ediately,
there
is a possibility that it m ay evaporate and then you would not be able to draw the solvent.
Photo 1: Chromatogram
Data Table 1: Chromatogram Data
Sample Solve nt front (mm)
Blue 1 48
Blue 2 48
Re d 3 48
Re d 40 48
Ye llow 5 48
Ye llow 6 48
UK
Copyrigh t 2019 - H an ds-On Labs | h t t p://h olscien ce.com 2/ 5
