Wing experiments on Drosophila Melanogaster

This lab report uses wing experiments to observe manipulations on substrates expressed by Drosophila in their wings. Also, it investigates mystery mutants using visual clues from their phenotype and patterns in embryogenesis and neurulation. A wing pattern experiment analyzed the effects due to a cut in the wing vein. It was discovered that a cut on the wing vein prevents formation of pigments. Another experiment to understand pigment formation involved placing detached wings in solutions containing their precursor substrates. It is found that male flies which normally form the spots, still form spots after artificially exposing them to these substrates. To investigate the identity of Drosophila mystery stocks, cuticle preparations were used and immunohistochemistry staining to match the phenotype and characteristics of the nervous system with that of the wild type. IHC is required because some mutants have similar phenotypes