Summary Molecular and cellular neuroscience
Molecular toolbox
Internal validity: Reliability and replicability
Face validity: The degree of descriptive similarity between the human
disorder and the animal model
Construct validity: ensures the model fulfills a theoretical rationale
Predictive validity (aka Criterion validity): allows extrapolation of the
effect of a particular manipulation from one species to another
External validity (Generalisability): the extent to which the results
obtained using a particular animal model can be generalized across
experimental conditions (i.e. different labs) and species.
Why use fly models?
- fast reproduction
- small and cheap (way cheaper than mice)
- easy to maintain
- easy to manipulate
- excellent genetic toolbox
(imprecise) P-element excision
, Bipartite UAS/GAL4 system
- enables time and tissue specific expression
- gene silencing using RNAi
GAL80ts
- temperature-controlled induction
- at 18* GAL80ts binds to GAL4 → gene silencing
- at 29* Gal80ts lets go off GAL4 → gene expression
Why mouse models?
- easy breading throughout year
- easy to maintain
- humans and mice both mammals (meer lichamelijke overeenkomst dan met vliegen)
- easy to obtain tissue to study
Molecular toolbox
Internal validity: Reliability and replicability
Face validity: The degree of descriptive similarity between the human
disorder and the animal model
Construct validity: ensures the model fulfills a theoretical rationale
Predictive validity (aka Criterion validity): allows extrapolation of the
effect of a particular manipulation from one species to another
External validity (Generalisability): the extent to which the results
obtained using a particular animal model can be generalized across
experimental conditions (i.e. different labs) and species.
Why use fly models?
- fast reproduction
- small and cheap (way cheaper than mice)
- easy to maintain
- easy to manipulate
- excellent genetic toolbox
(imprecise) P-element excision
, Bipartite UAS/GAL4 system
- enables time and tissue specific expression
- gene silencing using RNAi
GAL80ts
- temperature-controlled induction
- at 18* GAL80ts binds to GAL4 → gene silencing
- at 29* Gal80ts lets go off GAL4 → gene expression
Why mouse models?
- easy breading throughout year
- easy to maintain
- humans and mice both mammals (meer lichamelijke overeenkomst dan met vliegen)
- easy to obtain tissue to study
