BCHS 3201 LAB PRELAB QUESTIONS AND ANSWERS
The study of all of the proteins expressed in an organism at a particular time and/or set of conditions is
called: - CORRECT ANSWER✅✅Proteomics
In a microarray, to see what genes have been expressed (transcribed) by the cell under our set of
experimental conditions, we will purify ___________ from the organism: - CORRECT ANSWER✅✅mRNA
For our microarray project, the model organism under study is: - CORRECT ANSWER✅✅Arabidopsis
thaliana
How is the protective group removed to allow the addition of nucleotides to the probe on the DNA chip?
- CORRECT ANSWER✅✅UV light exposure
What is in a probe pair on an Affymetrix chip? - CORRECT ANSWER✅✅One probe to the specified gene
and one probe with a mismatch in the center of the probe to gauge non-specific hybridization levels
Which of the following represents a properly balanced centrifuge (check all that apply): - CORRECT
ANSWER✅✅a
How will the plasmid DNA be separated from chromosomal DNA in this plasmid purification lab? -
CORRECT ANSWER✅✅Differential centrifugation
What happens to plasmid DNA if you leave it in Solution II, lysis buffer, for too long? - CORRECT
ANSWER✅✅DNA Degrades
After lysing your E. coli cells and neutralizing the reaction, do you keep the supernatant or pellet for the
next step? - CORRECT ANSWER✅✅Supernatant
Which has more resolving power? - CORRECT ANSWER✅✅4% agarose gel
, What should you do with the liquid waste from this lab? - CORRECT ANSWER✅✅Bleach it for 20
minutes
Why must you wait 10 minutes after adding TE/RNase to the dry DNA pellet? - CORRECT
ANSWER✅✅To allow time for the RNA to be hydrolyzed or degraded
How will you mix the contents of the microcentrifuge tube after adding Solution II and Solution III to the
cell culture? - CORRECT ANSWER✅✅Pipet Gently
Where should you expect to see plasmid DNA after spinning it down out of solution? - CORRECT
ANSWER✅✅smeared up one side of the tube
What is agarose? - CORRECT ANSWER✅✅Seaweed
How do bacteria prevent their own DNA from being cleaved by restriction enzymes? - CORRECT
ANSWER✅✅By methylating their own bacterial DNA
Which of these could be a functional restriction site? - CORRECT ANSWER✅✅a
Which of the following pairs of enzymes is an example of a neoschizomer? - CORRECT ANSWER✅✅c
Which type of scenario is preferred when designing a cloning experiment so that the desired DNA insert
gets inserted in the correct orientation in the plasmid? - CORRECT ANSWER✅✅2 enzymes that leave
different sticky ends
Cleavage of sequences by a restriction endonuclease at slightly different sites than the recognition
sequence is called: - CORRECT ANSWER✅✅star activity
Seeing more or fewer bands of DNA on your gel than you expect after a restriction digest reaction of a
plasmid with known sequence can be the result of - CORRECT ANSWER✅✅-failure to digest all of the
target DNA in the sample
-no digestion
The study of all of the proteins expressed in an organism at a particular time and/or set of conditions is
called: - CORRECT ANSWER✅✅Proteomics
In a microarray, to see what genes have been expressed (transcribed) by the cell under our set of
experimental conditions, we will purify ___________ from the organism: - CORRECT ANSWER✅✅mRNA
For our microarray project, the model organism under study is: - CORRECT ANSWER✅✅Arabidopsis
thaliana
How is the protective group removed to allow the addition of nucleotides to the probe on the DNA chip?
- CORRECT ANSWER✅✅UV light exposure
What is in a probe pair on an Affymetrix chip? - CORRECT ANSWER✅✅One probe to the specified gene
and one probe with a mismatch in the center of the probe to gauge non-specific hybridization levels
Which of the following represents a properly balanced centrifuge (check all that apply): - CORRECT
ANSWER✅✅a
How will the plasmid DNA be separated from chromosomal DNA in this plasmid purification lab? -
CORRECT ANSWER✅✅Differential centrifugation
What happens to plasmid DNA if you leave it in Solution II, lysis buffer, for too long? - CORRECT
ANSWER✅✅DNA Degrades
After lysing your E. coli cells and neutralizing the reaction, do you keep the supernatant or pellet for the
next step? - CORRECT ANSWER✅✅Supernatant
Which has more resolving power? - CORRECT ANSWER✅✅4% agarose gel
, What should you do with the liquid waste from this lab? - CORRECT ANSWER✅✅Bleach it for 20
minutes
Why must you wait 10 minutes after adding TE/RNase to the dry DNA pellet? - CORRECT
ANSWER✅✅To allow time for the RNA to be hydrolyzed or degraded
How will you mix the contents of the microcentrifuge tube after adding Solution II and Solution III to the
cell culture? - CORRECT ANSWER✅✅Pipet Gently
Where should you expect to see plasmid DNA after spinning it down out of solution? - CORRECT
ANSWER✅✅smeared up one side of the tube
What is agarose? - CORRECT ANSWER✅✅Seaweed
How do bacteria prevent their own DNA from being cleaved by restriction enzymes? - CORRECT
ANSWER✅✅By methylating their own bacterial DNA
Which of these could be a functional restriction site? - CORRECT ANSWER✅✅a
Which of the following pairs of enzymes is an example of a neoschizomer? - CORRECT ANSWER✅✅c
Which type of scenario is preferred when designing a cloning experiment so that the desired DNA insert
gets inserted in the correct orientation in the plasmid? - CORRECT ANSWER✅✅2 enzymes that leave
different sticky ends
Cleavage of sequences by a restriction endonuclease at slightly different sites than the recognition
sequence is called: - CORRECT ANSWER✅✅star activity
Seeing more or fewer bands of DNA on your gel than you expect after a restriction digest reaction of a
plasmid with known sequence can be the result of - CORRECT ANSWER✅✅-failure to digest all of the
target DNA in the sample
-no digestion
