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Biology 110 Lab Practical #2, Bio 110 Lab Practical 2 Questions with Correct Answers

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Independent variable in the Chlorophyll Extraction Lab? - ANSWERSWavelength, light Dependent variable in the Chlorophyll Extraction Lab? - ANSWERSAbsorbency What type of driven reaction is photosynthesis? - ANSWERSLight driven reaction What is the relationship between energy and wavelength? - ANSWERSInverse. The greater the energy then the higher the frequency and shorter the wavelength. What light does chlorophyll absorb best? - ANSWERSBlue light What light does chlorophyll A absorb best? - ANSWERSRed 400-450 wavelength color - ANSWERSBlue 500-550 wavelength color - ANSWERSGreen 700-750 wavelength color - ANSWERSRed 3 solvents for Chlorophyl Extraction Lab? - ANSWERSPetroleum ether, ethanol, & methanol Chlorophyll in petroleum ether? - ANSWERSChlorophyll A Chlorophyll in ethanol? - ANSWERSCarotene Chlorophyll in methanol? - ANSWERSChlorophyll B What kind of density did petroleum ether have? - ANSWERSLow density Was chlorophyll A stored in the top layer or bottom layer in the test tube? - ANSWERSTop because it was in petroleum ether which had a low density. What kind of density did methanol have? - ANSWERSHigh density Was chlorophyll B stored in the top layer or bottom layer in the test tube? - ANSWERSBottom because it was in methanol which had a high density.

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2025/2026
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Biology 110 Lab Practical #2, Bio 110
Lab Practical 2 Questions with Correct
Answers
Independent variable in the Chlorophyll Extraction Lab? - ANSWERSWavelength, light

Dependent variable in the Chlorophyll Extraction Lab? - ANSWERSAbsorbency

What type of driven reaction is photosynthesis? - ANSWERSLight driven reaction

What is the relationship between energy and wavelength? - ANSWERSInverse. The
greater the energy then the higher the frequency and shorter the wavelength.

What light does chlorophyll absorb best? - ANSWERSBlue light

What light does chlorophyll A absorb best? - ANSWERSRed

400-450 wavelength color - ANSWERSBlue

500-550 wavelength color - ANSWERSGreen

700-750 wavelength color - ANSWERSRed

3 solvents for Chlorophyl Extraction Lab? - ANSWERSPetroleum ether, ethanol, &
methanol

Chlorophyll in petroleum ether? - ANSWERSChlorophyll A

Chlorophyll in ethanol? - ANSWERSCarotene

Chlorophyll in methanol? - ANSWERSChlorophyll B

What kind of density did petroleum ether have? - ANSWERSLow density

Was chlorophyll A stored in the top layer or bottom layer in the test tube? -
ANSWERSTop because it was in petroleum ether which had a low density.

What kind of density did methanol have? - ANSWERSHigh density

Was chlorophyll B stored in the top layer or bottom layer in the test tube? -
ANSWERSBottom because it was in methanol which had a high density.

, Independent variable of the Oxidation of Water Lab? - ANSWERSAmount of chloroplast
added

What does water split into? - ANSWERSO2 and H+

Dependent variable for the Oxidation of Water Lab? - ANSWERSRate at which water
was oxidized or the reduction of DPIP

Light hits which photosystem? - ANSWERSBoth

What is oxidation? - ANSWERSLoss of electrons

What is reduction? - ANSWERSGain of electrons

Is NADP+ reduced or oxidized? (Oxidation Lab) - ANSWERSOxidized

If DPIP is oxidized what happens? (Oxidation Lab) - ANSWERSIt is a blue color.

If DPIP is reduced what happens? (Oxidation Lab) - ANSWERSIt turns to clear.

What color wavelength does DPIP reflect? (Oxidation Lab) - ANSWERSBlue
wavelengths

mL of chloroplast (x) vs. rate of reaction (y) is what kind of relationship? (Oxidation Lab)
- ANSWERSDirect relationship, positive. More chloroplasts means a faster reaction.

Chloroplast has everything to make the reaction causing an increase in what?
(Oxidation Lab) - ANSWERSH2O oxidation

What two things play a factor in absorbance? (Oxidation Lab) - ANSWERSLight and
heat

Which factor (light or heat) played a larger role in driving the reaction? (Oxidation Lab) -
ANSWERSLight

What additive did the blank receive but the other solutions did not? (Oxidation Lab) -
ANSWERSBisulfide

What did bisulfide do in the blank? (Oxidation Lab) - ANSWERSIt changed the color to
clear

Independent variable in the Production of Oxygen Lab? - ANSWERSAmount of light
(PFR)

Dependent variable in the Production of Oxygen Lab? - ANSWERSRate of O2
production

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