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BIO 110 LAB QUIZ # 2 QUESTIONS WITH CORRECT ANSWERS

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Enzyme - ANSWERSa catalyst; a protein that speeds up a chm rxn by lowering amount of energy it takes to get the rxn going What is the reactant that enzyme acts on? - ANSWERSsubstrate What is the active site? - ANSWERSregion on enzyme where substrate binds Lock and Key - ANSWERSShape of active site and shape of substrate fit together like a lock and key( or a puzzle) Enzyme Action (5) - ANSWERS1) substrate enters active site 2) held by weak interactions 3) active site can lower energy activity and speed up rxn 4) converted to products 5) products released Enzymes are not what? - ANSWERSchanged up or used up in a chm rxn Are enzymes reusable? - ANSWERSYES A cell has approximately how many different enzymes? - ANSWERS1000 What is a catalyst? - ANSWERSanything that speeds up a rxn; rxn would be so much slower without it; without being used up in a rxn buffer - ANSWERSsubstance added to solution to resist changes in pH of solution temp and pH affect what? - ANSWERSenzyme activity What is denaturation? - ANSWERSenzyme is unraveled and is destroyed since it doesn't have an active site so they can't work anymore; losing its shape Can denaturation reverse? - ANSWERSNO What is the purpose of utilizing the Benedict's Test when examining the effect of concentration, temp, and pH on enzyme activity? - ANSWERSTo determine the presence of glucose or fructose which indicate activity of the enzyme Invertase. sucrose does not react so if there is a rxn taking place noticed by color change, the simple sugars are present.

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BIO 110 LAB QUIZ # 2 QUESTIONS
WITH CORRECT ANSWERS

Enzyme - ANSWERSa catalyst; a protein that speeds up a chm rxn by lowering amount
of energy it takes to get the rxn going

What is the reactant that enzyme acts on? - ANSWERSsubstrate

What is the active site? - ANSWERSregion on enzyme where substrate binds

Lock and Key - ANSWERSShape of active site and shape of substrate fit together like a
lock and key( or a puzzle)

Enzyme Action (5) - ANSWERS1) substrate enters active site
2) held by weak interactions
3) active site can lower energy activity and speed up rxn
4) converted to products
5) products released

Enzymes are not what? - ANSWERSchanged up or used up in a chm rxn

Are enzymes reusable? - ANSWERSYES

A cell has approximately how many different enzymes? - ANSWERS1000

What is a catalyst? - ANSWERSanything that speeds up a rxn; rxn would be so much
slower without it; without being used up in a rxn

buffer - ANSWERSsubstance added to solution to resist changes in pH of solution

temp and pH affect what? - ANSWERSenzyme activity

What is denaturation? - ANSWERSenzyme is unraveled and is destroyed since it
doesn't have an active site so they can't work anymore; losing its shape

Can denaturation reverse? - ANSWERSNO

What is the purpose of utilizing the Benedict's Test when examining the effect of
concentration, temp, and pH on enzyme activity? - ANSWERSTo determine the
presence of glucose or fructose which indicate activity of the enzyme Invertase. sucrose
does not react so if there is a rxn taking place noticed by color change, the simple
sugars are present.

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