Designing of novel
antigenic peptide
cocktail for the
detection of
antibodies to HIV-
1/2 by ELISA
Big Idea 13 Naeyaert Justine 1
, Natuurwetenschappen 3
Inhoud
I. Lijst met afkortingen en symbolen .................................................................................................. 3
II. Verklarende woordenlijst ................................................................................................................ 4
III. Lijst met tabellen ......................................................................................................................... 5
IV. Lijst met figuren........................................................................................................................... 5
1. Inleiding ....................................................................................................................................... 6
2. Humaan Immunodeficiëntie Virus .............................................................................................. 6
2.1. Wat is HIV? .......................................................................................................................... 6
2.2. Oorzaken en gevolgen ......................................................................................................... 7
2.2.1. Hoe krijg je HIV? .......................................................................................................... 7
2.2.2. Gevolgen van HIV ........................................................................................................ 7
3. HIV opsporen ............................................................................................................................... 7
4. Nieuwe detectiemethode door ELISA-techniek .......................................................................... 8
4.1. Het materiaal en de methodes............................................................................................ 8
4.1.1. Het ontwikkelen van de cocktail ................................................................................. 8
4.2. HPLC-zuivering en sequentieanalyse................................................................................... 8
4.2.1. De ELISA-techniek ...................................................................................................... 10
4.3. Resultaten.......................................................................................................................... 11
4.4. Conclusie ........................................................................................................................... 12
VI. Referentielijst ............................................................................................................................ 13
VII. Bijlage ........................................................................................................................................ 14
Big Idea 13 Naeyaert Justine 2
, Natuurwetenschappen 3
I. Lijst met afkortingen en symbolen
Ab antibody
Ag antigen
AIDS acquired immuno deficiency syndrome
BSA bovine serum albumin
C cysteine bridge
DCM dichloro methane
DCMS dimethyl sulfoxide
Elisa enzyme linked immunosorbent assay
Env envelope
Gp glycoproteins
H2O2 hydrogen peroxide
HIV human immuno-deficiency virus
HPCL high pressure liquid chromatography
HRPO horseradish peroxidase
M molar
Mm millimolar
MNc mean of negative control
MPc mean of positive control
N normal
NC negative control
NHS normal human serum
Nm nanometer
NRS normal rabbit serum
OD optical density
PBS phosphate buffered saline
PC positive control
Rp24 recombinant protein
TFAA trifluoro acetic acid
TMB 3,3,5,5-tetramethyl-benzedine
v/v volume by volume
WB Western blot
WHO World Health Organization
µl micro-liter
PrEP Pre Exposure Profylaxis
Big Idea 13 Naeyaert Justine 3
antigenic peptide
cocktail for the
detection of
antibodies to HIV-
1/2 by ELISA
Big Idea 13 Naeyaert Justine 1
, Natuurwetenschappen 3
Inhoud
I. Lijst met afkortingen en symbolen .................................................................................................. 3
II. Verklarende woordenlijst ................................................................................................................ 4
III. Lijst met tabellen ......................................................................................................................... 5
IV. Lijst met figuren........................................................................................................................... 5
1. Inleiding ....................................................................................................................................... 6
2. Humaan Immunodeficiëntie Virus .............................................................................................. 6
2.1. Wat is HIV? .......................................................................................................................... 6
2.2. Oorzaken en gevolgen ......................................................................................................... 7
2.2.1. Hoe krijg je HIV? .......................................................................................................... 7
2.2.2. Gevolgen van HIV ........................................................................................................ 7
3. HIV opsporen ............................................................................................................................... 7
4. Nieuwe detectiemethode door ELISA-techniek .......................................................................... 8
4.1. Het materiaal en de methodes............................................................................................ 8
4.1.1. Het ontwikkelen van de cocktail ................................................................................. 8
4.2. HPLC-zuivering en sequentieanalyse................................................................................... 8
4.2.1. De ELISA-techniek ...................................................................................................... 10
4.3. Resultaten.......................................................................................................................... 11
4.4. Conclusie ........................................................................................................................... 12
VI. Referentielijst ............................................................................................................................ 13
VII. Bijlage ........................................................................................................................................ 14
Big Idea 13 Naeyaert Justine 2
, Natuurwetenschappen 3
I. Lijst met afkortingen en symbolen
Ab antibody
Ag antigen
AIDS acquired immuno deficiency syndrome
BSA bovine serum albumin
C cysteine bridge
DCM dichloro methane
DCMS dimethyl sulfoxide
Elisa enzyme linked immunosorbent assay
Env envelope
Gp glycoproteins
H2O2 hydrogen peroxide
HIV human immuno-deficiency virus
HPCL high pressure liquid chromatography
HRPO horseradish peroxidase
M molar
Mm millimolar
MNc mean of negative control
MPc mean of positive control
N normal
NC negative control
NHS normal human serum
Nm nanometer
NRS normal rabbit serum
OD optical density
PBS phosphate buffered saline
PC positive control
Rp24 recombinant protein
TFAA trifluoro acetic acid
TMB 3,3,5,5-tetramethyl-benzedine
v/v volume by volume
WB Western blot
WHO World Health Organization
µl micro-liter
PrEP Pre Exposure Profylaxis
Big Idea 13 Naeyaert Justine 3
