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Sterile Processing Certification Exam – 300+ Q&A | Decontamination, Sterilization, Microbiology |

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This comprehensive study guide contains over 300 up-to-date questions and verified answers tailored for the Sterile Processing Certification Exam (2025/2026). It provides in-depth, high-yield content aligned with current industry standards, including AAMI, OSHA, and CDC guidelines. The document is structured as a question-and-answer format, ideal for review and mastery of core competencies in central sterile processing and healthcare reprocessing workflows. Topics thoroughly covered include: Environmental Standards: Temperature, humidity, air pressure, and air exchanges for decontamination, sterile storage, and packaging areas Decontamination Protocols: Manual/automated cleaning, enzymatic detergents, bioburden removal, disinfection levels, equipment care Sterilization Techniques: Steam, EO, LTGP, ozone, dry heat, biological and chemical indicators, and monitoring processes (e.g., Bowie Dick test, PCDs) Instrument Care & Handling: Disassembly, passivation, lubrication, packaging principles, visual inspection, surgical instrument anatomy Microbiology & Infection Control: Bacteria types and shapes, Gram staining, spores, prions, transmission routes, HAI prevention, hand hygiene Anatomy & Physiology Basics: Key structural and functional systems relevant to SPD technicians (skeletal, muscular, nervous, immune, urinary, etc.) Inventory & Distribution Systems: FIFO, case cart systems, demand and par level distribution, storage guidelines, stock rotation Regulatory Compliance: HIPAA, FDA, OSHA, and facility-based safety and exposure protocols This document is ideal for: Sterile Processing Technician students (CSPDT, CRCST exam prep) Surgical Technology and Central Service training programs Healthcare professionals in infection prevention, operating room, and medical equipment reprocessing roles Certification seekers in programs by IAHCSMM (now HSPA) and CBSPD Keywords: sterile processing exam, SPD certification, decontamination area, packaging area conditions, steam sterilization, EO sterilizer, high-level disinfection, surgical instruments, prions and spores, Bowie Dick test, biological indicators, AAMI standards, OSHA compliance, infection control, central supply technician, microbiology review

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Sterile Processing Study Material for
Certification Exam 2025/2026 Exam
Questions and Verified Answers |
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What is the Area Temp & Humidity for Decontamination? - 🧠ANSWER

✔✔60 deg to 65 deg F, 30% to 60% Humidity


What is the air exchange rate & pressure type for Decontamination? -

🧠ANSWER ✔✔10 air exchanges per hour under Negative Pressure


What is the Area Temp & Humidity for the Sterile Storage Area? -

🧠ANSWER ✔✔68 deg to 75 deg F, 35% to 70% Humidity


What is the Area Temp & Humidity for the Prep & Packaging Area? -

🧠ANSWER ✔✔68 deg to 73 deg F, Ideal Humidity 50% - not lower than

35%

,The main role of sterile processing is to: - 🧠ANSWER ✔✔Provide

dependable reliable services to enhance the quality of patient care.


Ethics is the practice of: - 🧠ANSWER ✔✔Doing the right thing.


Which of the following establishes sterilization practice standards based on

opinion, research, and/or theory: - 🧠ANSWER ✔✔AAMI


EPA, FDA, OSHA and state departments of health (DOH) primarily issue: -

🧠ANSWER ✔✔Regulations.


Standards for CS/SPD practice are written by: - 🧠ANSWER ✔✔AAMI


The Health Insurance Portability and Accountability Act provides for which

ethical issue? - 🧠ANSWER ✔✔Confidentiality


If healthcare facilities reprocess single-use devices, the devices must be

cleared by: - 🧠ANSWER ✔✔FDA


OSHA has a great effect on CS/SPD by its regulation of occupational

exposure to airborne contaminants, exposure from bloodborne pathogens,

and which of the following: - 🧠ANSWER ✔✔Communication of workplace

hazards

,The Medical Device Reporting regulation requires: - 🧠ANSWER

✔✔Healthcare facilities to report deaths or serious injuries from medical

devices.

Collection of data and identification of issues are part of what kind of

program? - 🧠ANSWER ✔✔Quality Assurance


To ensure that all employees are learning the same information, training

should be: - 🧠ANSWER ✔✔Standardized


Which branch of science refers to the form and organization of body parts?

- 🧠ANSWER ✔✔Anatomy


Which branch of science refers to the function of specific body parts? -

🧠ANSWER ✔✔Physiology


The smallest and most numerous structural units possessing the basic

characteristics of life are called: - 🧠ANSWER ✔✔Cells


Which of the following is NOT an environmental factor required for the life

of a cell? - 🧠ANSWER ✔✔Cold


DNA is found in which part of the cell? - 🧠ANSWER ✔✔Nucleus




3
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STATEMENT. ALL RIGHTS RESERVED

, Complex structures with specialized functions within the body are known

as: - 🧠ANSWER ✔✔Organs


Red blood cells develop in which part of the body? - 🧠ANSWER ✔✔Bone

Marrow


The smallest bones of the body are found in the: - 🧠ANSWER ✔✔Middle

Ear

Which of the following supports the tongue and the functions in

swallowing? - 🧠ANSWER ✔✔Hyoid Bone


The skull, hyoid, vertebral column, and thoracic cage are part of the: -

🧠ANSWER ✔✔Axial Skeleton


Muscles make up what part of the body's bulk? - 🧠ANSWER ✔✔1/2


Which of the following bones can be found in the axial skeleton? -

🧠ANSWER ✔✔Sternum


Which of the following is part of the appendicular skeleton? - 🧠ANSWER

✔✔Humerus


The femur, tibia, and fibula are bones of the: - 🧠ANSWER ✔✔Lower limbs

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