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Conquer ASCP MLS Exam: Ultimate Questions & Answers Study Guide for 100% Pass in 2025/2026

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Hey, medical lab science students! Ready to ace the ASCP Medical Laboratory Scientist (MLS) exam? This 2025/2026 study guide is your all-in-one resource, loaded with 100% correct questions and answers covering key areas like clinical chemistry, microbiology, hematology, immunology, and lab operations. Designed for busy students, it breaks down complex topics with clear explanations and practical insights to boost your confidence. Perfect for quick reviews or comprehensive prep, this guide ensures you’re fully equipped to pass the ASCP MLS exam on your first try. Start studying now and launch your lab career!

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ASCP MLS QUESTIONS AND ANSWERS
100% PASS
What feature is most often found with patients with a splenectomy on a Wright's stained blood

smear? ✔✔Howell-Jolly bodies



Patients with antibody to the following antigen are immune to Hepatitis B: ✔✔Surface antigen

(HbSAg)



Which two of the following tests are helpful for documenting previous Streptococcal throat and

skin infections: ✔✔ASO titer and Anti-DNase B



In mass spectrometry, the "appearance potential" is defined as the: ✔✔Minimum energy required

for the appearance of a particular fragment ion in mass spectrum



What is generally accepted as the lower threshold value for semen pH from fertile males? ✔✔7.2



Coulometry: ✔✔An automated method for measuring chloride which generates silver ions in the

reaction

,Erythroleukemia: ✔✔Also known as Acute Myelogenous Leukemia type M6. This type is

associated with either the presence of both erythroid and myeloid precursors OR strictly

erythroid precursors.



Myelomonocytic leukemia ✔✔Is associated with an increase in myeloid and monocytic cell

precursors, but not red blood cell precursors



Species that are Lysine (+), arginine (=) and ornithine (+): ✔✔Enterobacter aerogenes,

Edwardsiella species and most Serratia species



Lysine reaction for all Citrobacter: ✔✔Negative



Key identifying characteristic for Pantoa (Enterobacter) agglomerans: ✔✔negative reactions for

the three decarboxylases commonly tested ( Lysine, arginine and ornithine)



Which organism is MOST likely responsible for septic reactions associated with Red Blood Cell

transfusions? ✔✔Yersina entercolitica is most likely responsible for septic reactions in

transfusions of Red Blood Cells. This organism is usually acquired by ingestion of contaminated

food and causes mild symptoms of abdominal pain and diarrhea. Growth of Y. entercolitica is

enhanced in iron-rich environments such as red cell components.

, Gaucher's disease ✔✔The most common lysosomal storage disease caused by insufficient

activity of the lysosomal enzyme glucocerebrosidase that leads to the deposition of

glucocerebroside in cells of the macrophage-monocyte system.



Diseases associated with dysfunctions of polymorphonuclear neutrophils (PMNs) ✔✔Chediak-

Higashi syndrome (CH), Chronic granulomatous disease (CGD), Myeloperoxidase deficiency

(MPO)



Nephrotic Syndrome: ✔✔Caused by damage to the kidneys, especially the basement membrane

of the glomerulus; which causes abnormal excretion of protein and red blood cells in the urine.

Fats are also present in the urine in most cases. A foamy appearance of the urine is a key

characteristic of this condition.



Clinical symptoms include:

Swelling

Weight gain from fluid retention

Poor appetite

High blood pressure



Key Biochemical reactions for Enterobacteriaceae: ✔✔The key biochemical reactions by which

the family Enterobacteriaceae can be identified include fermentation of carbohydrates, reduction

of nitrates to nitrites and the absence of cytochrome oxidase activity.
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