BACTERIAL GROWTH VS ANTIBIOTICS 1
Bacterial growth versus the presence of various antibiotics
Unnamed A. Student
xxxxxx University
, BACTERIAL GROWTH VS ANTIBIOTICS 2
Bacterial growth versus the presence of various antibiotics
Introduction
The purpose of this lab report is to compare the bacterial growth of different species of
bacteria when placed in environments with various kinds of antibiotics. The bacteria Bacillus
subtilis and Pseudomonas aeruginosa were used. The antibiotics for the experiment were dish
soap, hydrogen peroxide and mouthwash. Water was used as a control. The hypotheses for this
experiment were that (1) dish soap would be most effective and mouthwash would be the least
effective in killing the B. subtilis bacterium and (2) mouthwash would be most effective and dish
soap would be the least effective in killing the P. aeruginosa bacterium.
Bacillus subtilis is a gram-positive, rod-shaped bacterium is a facultative anaerobe. They
live in soil and water. They can cause spoilage in canned foods. This species is not pathogenic to
humans, though humans can still be a host organism. Other Bacillus species that are pathogenic
can cause anthrax in humans and animals and produces a toxin that can cause disease in insects
(Todar, 2008).
Pseudomonas aeruginosa is a gram-negative, rod-shaped bacterium that is an obligate
aerobe. It can be found in soil, water, skin flora and some man-made environments. It is an
opportunistic pathogen, meaning it infects compromised tissues. It causes urinary tract
infections, dermatitis, respiratory system and gastrointestinal infections (Todar, 2008).
For this experiment, each bacterium was placed in a separate petri plate. The plate was
divided into quadrants. Once the bacteria were transferred to the plates, the three antibiotics and
the control of water were introduced; one to each quadrant of the plate. The plates were sealed
and placed in a 37 degree Celsius incubator for 24 hours.
Bacterial growth versus the presence of various antibiotics
Unnamed A. Student
xxxxxx University
, BACTERIAL GROWTH VS ANTIBIOTICS 2
Bacterial growth versus the presence of various antibiotics
Introduction
The purpose of this lab report is to compare the bacterial growth of different species of
bacteria when placed in environments with various kinds of antibiotics. The bacteria Bacillus
subtilis and Pseudomonas aeruginosa were used. The antibiotics for the experiment were dish
soap, hydrogen peroxide and mouthwash. Water was used as a control. The hypotheses for this
experiment were that (1) dish soap would be most effective and mouthwash would be the least
effective in killing the B. subtilis bacterium and (2) mouthwash would be most effective and dish
soap would be the least effective in killing the P. aeruginosa bacterium.
Bacillus subtilis is a gram-positive, rod-shaped bacterium is a facultative anaerobe. They
live in soil and water. They can cause spoilage in canned foods. This species is not pathogenic to
humans, though humans can still be a host organism. Other Bacillus species that are pathogenic
can cause anthrax in humans and animals and produces a toxin that can cause disease in insects
(Todar, 2008).
Pseudomonas aeruginosa is a gram-negative, rod-shaped bacterium that is an obligate
aerobe. It can be found in soil, water, skin flora and some man-made environments. It is an
opportunistic pathogen, meaning it infects compromised tissues. It causes urinary tract
infections, dermatitis, respiratory system and gastrointestinal infections (Todar, 2008).
For this experiment, each bacterium was placed in a separate petri plate. The plate was
divided into quadrants. Once the bacteria were transferred to the plates, the three antibiotics and
the control of water were introduced; one to each quadrant of the plate. The plates were sealed
and placed in a 37 degree Celsius incubator for 24 hours.