MODULE 3 EXAM
Actual Questions and Verified Answers
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Portage Learning, Geneva College
Inside you will get:
➢ Module 3 Exam
➢ True & False Questions
➢ Multiple Choice Questions and Answers
➢ Clear images
➢ Expert-Verified Explanations
,1. Resolution and contrast are two critical factors that influence your
ability to see an object. Explain each.
Correct Answer:
Resolution is the microscope’s ability to distinguish two points as separate
entities; it depends on the wavelength of light and the numerical aperture of
the objective lens. Poor resolution causes objects to appear merged.
Contrast is the difference in light intensity between the specimen and the
background; sufficient contrast is needed to differentiate features within or
between cells. Without adequate contrast, specimens may blend with the
background, making features indistinct.
Expert Rationale:
Improving resolution allows visualization of fine structural details, critical for
examining small microbes or subcellular components. Contrast
enhancement techniques, like staining or specialized microscopy (e.g.,
phase contrast), make less visible organisms or structures discernible,
highlighting microbiological function or morphology.
2. A micrometer is defined as:
(one millionth of a meter, 10⁻⁶)
Answer choices:
A. 10⁻³
B. 10⁻⁶
,C. 10⁻⁹
D. 10⁻¹²
Correct Answer: B. 10⁻⁶
Expert Rationale:
A micrometer (µm) is one-millionth of a meter (10⁻⁶ m) and is the scale
commonly used to measure bacteria, protozoa, and many cellular
organelles. Distinguishing between micrometers and nanometers is crucial
for grasping organism sizes and imaging limits in microbiology.
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3. True or False: A nanometer (10⁻⁹) is longer than a micrometer (10⁻⁶).
Answer choices:
True
False
Correct Answer: False
Expert Rationale:
A nanometer is 1,000 times smaller than a micrometer, because 10⁻⁹ <
10⁻⁶. This scale difference has practical implications in microscopy and
biological measurements, affecting what can be visualized with different
microscopy techniques.
4. A nanometer is defined as:
, A. 10⁻³
B. 10⁻⁶
C. 10⁻⁹
D. 10⁻¹²
Correct Answer: C. 10⁻⁹
Expert Rationale:
A nanometer (nm) is one-billionth of a meter (10⁻⁹ m). This ultra-small scale
is critical when discussing viruses, molecular structures, and
ultramicroscopic details that standard light microscopes cannot resolve.
Understanding metric prefixes is fundamental in microbiology since many
cellular and molecular dimensions fall within the nanometer scale.
5. Define the measurements micrometer and nanometer.
Correct Answer:
A micrometer (µm) is defined as one-millionth of a meter (10⁻⁶ m). A
nanometer (nm) is one-billionth of a meter (10⁻⁹ m).
Expert Rationale:
Differentiating µm and nm scales is fundamental in microbiology to
contextualize the size range of organisms (bacteria in µm) versus
molecular or virus sizes (nm scale). These units permit standardized
communication of size in microscopy and cellular biology.