BOC Microbiology Fungi Exam Questions with
Revised Answers| Verified 100% Correct
Which of the following reagents should be used as a mucolytic, alkaline reagent for
digestion and decontamination of a sputum for mycobacterial culture? - ✔✔N-acetyl-
L-cystine and NaOH
The function of N-acetyl-L-cysteine in the reagent for acid fast digestion-
decontamination procedure is to: - ✔✔liquefy the mucus
When staining acid-fast bacilli with Truant autamine-rhodamine stain, potassium
permanganate is used as a: - ✔✔quenching agent.
Middlebrook 7H10 and 7H11 media must be refrigerated in the dark, and incubated in
the dark as well. If these conditions are not met, the media may prove toxic for
mycobacteria because: - ✔✔Formaldehyde may be produced.
The method used for processing specimens for mycobacterial culture contaminated
with Pseudomonas is: - ✔✔Oxalic acid.
An AFB broth culture is positive for acid-fast bacilli at 1 week while the agar slant shows
no growth. The most likely explanation for this is: - ✔✔AFB grow more rapidly in
liquid media.
A bronchial washing is processed for acid fast bacilli. Which of the following precautions
should be taken in order to prevent infection of laboratory personnel? - ✔✔Process
all specimens in a biological safety hood.
Tubercle bacilli are specifically stained by: - ✔✔Carbol fuchsin
, The disease-producing capacity of Mycobacterium tuberculosis depends primarily upon:
- ✔✔Capacity to withstand intracellular digestion by macrophages.
Which species of Mycobacterium includes a BCG strain used for vaccination against
tuberculosis? - ✔✔bovis
AFB smear positive respiratory specimens may be reliably identified as Mycobacterium
tuberculosis the same day the smear was read by: - ✔✔Nucleic acid amplification.
Which of the following is considered a primary drug for the treatment of
Mycobacterium tuberculosis? - ✔✔Rifampin
The mycobacteria that produce a deep yellow or orange pigment both in the dark and
light are: - ✔✔Scotochromogens
Mycobacteria that produce pigment only after exposure to light are classified as: -
✔✔photochromogens
In a suspected case of Hansen disease (leprosy), a presumptive diagnosis is established
by: - ✔✔Detection of weakly acid-fast bacilli in infected tissue.
The best medium for culture of Mycobacterium tuberculosis is: - ✔✔Lowenstein-
Jensen medium
Photochromogens produce pigment when: - ✔✔Exposed to light for 1 hour.
Revised Answers| Verified 100% Correct
Which of the following reagents should be used as a mucolytic, alkaline reagent for
digestion and decontamination of a sputum for mycobacterial culture? - ✔✔N-acetyl-
L-cystine and NaOH
The function of N-acetyl-L-cysteine in the reagent for acid fast digestion-
decontamination procedure is to: - ✔✔liquefy the mucus
When staining acid-fast bacilli with Truant autamine-rhodamine stain, potassium
permanganate is used as a: - ✔✔quenching agent.
Middlebrook 7H10 and 7H11 media must be refrigerated in the dark, and incubated in
the dark as well. If these conditions are not met, the media may prove toxic for
mycobacteria because: - ✔✔Formaldehyde may be produced.
The method used for processing specimens for mycobacterial culture contaminated
with Pseudomonas is: - ✔✔Oxalic acid.
An AFB broth culture is positive for acid-fast bacilli at 1 week while the agar slant shows
no growth. The most likely explanation for this is: - ✔✔AFB grow more rapidly in
liquid media.
A bronchial washing is processed for acid fast bacilli. Which of the following precautions
should be taken in order to prevent infection of laboratory personnel? - ✔✔Process
all specimens in a biological safety hood.
Tubercle bacilli are specifically stained by: - ✔✔Carbol fuchsin
, The disease-producing capacity of Mycobacterium tuberculosis depends primarily upon:
- ✔✔Capacity to withstand intracellular digestion by macrophages.
Which species of Mycobacterium includes a BCG strain used for vaccination against
tuberculosis? - ✔✔bovis
AFB smear positive respiratory specimens may be reliably identified as Mycobacterium
tuberculosis the same day the smear was read by: - ✔✔Nucleic acid amplification.
Which of the following is considered a primary drug for the treatment of
Mycobacterium tuberculosis? - ✔✔Rifampin
The mycobacteria that produce a deep yellow or orange pigment both in the dark and
light are: - ✔✔Scotochromogens
Mycobacteria that produce pigment only after exposure to light are classified as: -
✔✔photochromogens
In a suspected case of Hansen disease (leprosy), a presumptive diagnosis is established
by: - ✔✔Detection of weakly acid-fast bacilli in infected tissue.
The best medium for culture of Mycobacterium tuberculosis is: - ✔✔Lowenstein-
Jensen medium
Photochromogens produce pigment when: - ✔✔Exposed to light for 1 hour.
