Biochemistry
Dna Chemistry And Analysis
Compiled By Simon Mwangi
Edition: 2024/25
, Science | Biochemistry I of VI pages
1. What are the 4 components of a nucleotide?
nitrogenous base (purine or pyrimidine)
sugar (ribose or deoxyribose)
phosphate
rare or minor bases
2. Methylation is an important modification to a major base because ___ ___ can be controlled by this
gene expression
3. Transfer RNA contains a high percentage of ___ bases
minor
4. Ribosomal RNA contains a high percentage of ____ bases
methylated
5. What is the most commonly modified base in DNA?
5-methylcytosine
6. In regards to sugar, DNA contains ____ and RNA contains ____
B-2-deoxyribose; B-D-ribose
7. DNA contains thymine while RNA contains ____ in place of it
Uracil
8. In regards to linkage, mononucleotides are joined together by ____ bridges between the 5hydroxyl group of
phosphodiester
9. In regards to linkage, the bases are not directly attached covalently to one another. True or false?
true
10. Nucleic acid sequences are always written from left to right: ___ to ___ direction
5;3
11. The products of strong acid hydrolysis of DNA or RNA are what?
pyrimidine nucleosides, deoxyribose or ribose and phosphate
12. Base hydrolysis of ____ proceeds through a 2,3-cyclic phosphate intermediate
RNA
13. Since DNA doesnt contain a 2 hydroxyl group, it is resistant to ____ hydrolysis.
base
note: this is important method for separating DNA from RNA
14. DNA is stable in base and RNA is not stable. True or false?
true
15. What is the product of base hydrolysis of RNA?
Biochemistry 2024/25 Edition
Dna Chemistry And Analysis
Compiled By Simon Mwangi
Edition: 2024/25
, Science | Biochemistry I of VI pages
1. What are the 4 components of a nucleotide?
nitrogenous base (purine or pyrimidine)
sugar (ribose or deoxyribose)
phosphate
rare or minor bases
2. Methylation is an important modification to a major base because ___ ___ can be controlled by this
gene expression
3. Transfer RNA contains a high percentage of ___ bases
minor
4. Ribosomal RNA contains a high percentage of ____ bases
methylated
5. What is the most commonly modified base in DNA?
5-methylcytosine
6. In regards to sugar, DNA contains ____ and RNA contains ____
B-2-deoxyribose; B-D-ribose
7. DNA contains thymine while RNA contains ____ in place of it
Uracil
8. In regards to linkage, mononucleotides are joined together by ____ bridges between the 5hydroxyl group of
phosphodiester
9. In regards to linkage, the bases are not directly attached covalently to one another. True or false?
true
10. Nucleic acid sequences are always written from left to right: ___ to ___ direction
5;3
11. The products of strong acid hydrolysis of DNA or RNA are what?
pyrimidine nucleosides, deoxyribose or ribose and phosphate
12. Base hydrolysis of ____ proceeds through a 2,3-cyclic phosphate intermediate
RNA
13. Since DNA doesnt contain a 2 hydroxyl group, it is resistant to ____ hydrolysis.
base
note: this is important method for separating DNA from RNA
14. DNA is stable in base and RNA is not stable. True or false?
true
15. What is the product of base hydrolysis of RNA?
Biochemistry 2024/25 Edition
