Molecular Diagnostics Exam 2 Questions With Complete Answers 2024

Molecular Diagnostics Exam 2 Questions
With Complete Answers 2024



Enzyme .that .digests .nucleic .acid .that .starts .at .5' .or .3' .and .extends .inward .- .correct
.answer.Exonuclease




Enzyme .that .digests .nucleic .acid .at .a .specific .sequence .within .the .DNA .molecule .-
.correct .answer.Endonuclease




A .virus .that .infects .bacteria .- .correct .answer.Bacteriophage

Produced .by .bacteria .and .help .to .protect .against .infection .by .bacteriophage .- .correct
.answer.Restriction .Endonuclease




True .or .False: .Restriction .endonucleases .will .work .on .all .DNA .regardless .of .source .-
.correct .answer.True




How .many .type .of .Restriction .Enzymes .are .there? .- .correct .answer.3

Which .is .the .most .useful .Restriction .enzyme .for .molecular .techniques? .- .correct
.answer.Type .II




What .is .the .function .of .restriction .enzymes .in .molecular .biology? .- .correct
.answer."Molecular .Scissors"




How .many .base .pairs .do .restriction .enzymes .recognize? .- .correct .answer.4 .to .6 .base
.pairs .in .palindromic .sequences




What .factors .determine .how .a .restriction .enzyme .is .identified? .- .correct .answer.-Bacterial
.genus .& .species

-Strain
-Enzyme .number

What .are .the .2 ."cuts" .that .restriction .enzymes .will .make .in .the .DNA? .- .correct .answer.-
"Sticky" .End
-"Blunt" .End

Restriction .enzyme .that .makes .a ."blunt" .cut .between .the .G .and .C .in:
5-GGCC-3' .- .correct .answer.HAE .III

, HAE .III .restriction .enzyme .is .derived .from .what .micro-organism? .- .correct
.answer.Haemophilus .aegypticus




Restriction .enzyme .that .makes .a ."blunt" .cut .between .the .G .and .C .in:
5'-CAGCTG-3' .- .correct .answer.Pvu .II

Pvu .II .restriction .enzyme .is .derived .from .what .micro-organism? .- .correct .answer.Proteus
.vulgaris




Restriction .enzyme .that .makes .a ."sticky" .cut .between .the .A .and .G .in:
5'-GAATTC-3' .- .correct .answer.Eco .R1 .Enzyme

Eco .R1 .restriction .enzyme .is .derived .from .what .micro-organism? .- .correct .answer.E. .coli

Restriction .enzyme .that .makes .a ."sticky" .cut .between .the .G .and .A .in:
5'-GACGTC-3' .- .correct .answer.Pst .I .Enzyme

Pst .I .restriction .enzyme .is .derived .from .what .micro-organism? .- .correct
.answer.Providencia .stuartii




Restriction-Fragment .Length .Polymorphisms .(RFLPs) .- .correct .answer.DNA .fragments
.produced .by .restriction .enzymes




What .are .RFLPs .used .for? .- .correct .answer.-DNA .profiling
-Diagnosing .genetic .diseases
-Micro-organism .fingerprinting

If .a .DNA .strand .is .short, .would .there .be .more .or .less .recognition .sites .along .the .strand? .-
.correct .answer.More




What .is .the .average .distance .between .cuts? .- .correct .answer.4^n, .where .n .is .the .number
.of .base .pairs .in .the .recognition .site




Extrachromosomal .DNA .- .found .in .many .bacteria .- .correct .answer.Plasmid

What .is .the .correct .order .for .preparing .recombinant .DNA .- .correct .answer.-Plasmid
.isolated

-DNA .w/ .gene .cleaved .by .enzyme
-Gene .inserted .into .Plasmid, .creating .recombinant .DNA
-Plasmid .taken .up .by .Cell
-Cells .cloned
-Product .harvested

What .genetic .products .are .harvested .by .recombinant .DNA .technology .- .correct .answer.-
Insulin