BOC Exam Review – Micro Pro Test
Questions Fully Answered.
Rejection of inappropriately collected or transported specimens:
A) Is the sole responsibility of the laboratory and should be done without regard to physician requests
B) Should be initiated by laboratory personnel but approved by nursing or physician staff responsible for
the care of the patient
C) Is not in the best interest of patient care and should not be considered appropriate
D) Is the responsibility of nursing staff and should not be an issue for laboratory personnel - Answer B)
Should be initied by laboratory personnel but approved by nursing or physician staff responsible for the
care of the patient
Specimens for mcirobiologic studies should:
A) Be transported to the laboratory within 30 minutes of collection
B) Be contained in specimen containers that are leak-proof
C) SHould be transported within sealable, leak-proof plastc bags with a separate section for paperwork
D) Be clearly marked with a biohazard label
E) All of the above are correct - Answer E) All of the above are correct
Holding media such as Stuart's medium and Amie's medium are used for? - Answer Maintaining the
viability of microorgansims present in a specimen
Direct examinations such as the Gram stain serve what purpose(s)?
A) The quality of the specimen can be assessed
B) To give the physician an early indication of what may be wrong with the patient
C) The workup of the specimen can be guided
D) All of the above are correct - Answer D) All of the above are correct
Which type of media is designed to support the growth of one group of organisms, but not another, by
containing antimicbrobials, dyes, or alcohol? - Answer Selective
,Reduced O2 (5% to 10%) and increased CO2 (8% to 10%) is the environmental condition that best suuits
which type of organism? - Answer Microaerophiles
The agency that reuiqres helath care facilities to provide employees with all devices and mechanisms
necessary to protect them from hazards encountered in the laboratory is? - Answer OSHA
Procedures such as grinding, mincing, vortexing, and preparing smears:
A) Are hazardous and should be done wearing gloves and gown protection
B) Create aerosol droplets and should be performed only in an approved biosafety cabinet
C) Should be avoided with speciments known to contain infectious agents
D) Produce a spill hazard and should be done over an absortive mat
E) All of the above are correct - Answer E) All of the above are correct
The autoclave sterilizes material by: - Answer Applying moist heat at high temperatures and increased
pressure (121 C for 15 minutes at 15 PSI)
In microscopy, resolution can be defined as:
A) The magnification power
B) The extent to which detail in the magnified object is maintained
C) The ability of objects to stand out from the background
D) All of the above are correct - Answer B) The extent to which detail in the magnified object is
maintained
A mordant that is applied after the primary stain to chemically bond the alkaline dye to the bacterial cell
wall is? - Answer Gram's iodine
Which of the following statements are true concerning darkfiel microscopy?
A) This type of microscopy is used in some laboratories to detect spirochetes
B) The condenser does not allow light to pass directly through the specimen but directs the light t ohit
the specimen at an oblique angle
, C) THis technique involves the alteration of microscopic technique rather than the use of dyes or stains
to achieve contrast
D) All of the above are correct - Answer D) All of the above are correct
Organisms that have relatively complex nutritional needs, requiring extra media componenets to be used
for growth, are called? - Answer Fastidious
A bacterial colony can be described as:
A) Sufficiently large numbers of a bacterium that can be observable with the unaided eye
B) Derived from a single bacterial cell
C) Belonging to the same genus and species
D) Having identical genetic and phenotypic characteristics
E) All of the above are correct - Answer E) All of the above are correct
Which category of media can be described as containing nutrients that support growth of most
nonfastidious organisms without giving any particular organism as growth advantage?
A) Enrichment
B) Supportive
C) Selective
D) Differential - Answer B) Supportive
Which category of media can be described as containing one or more agents that are inhibitory to all
organisms except those being sought?
A) Enrichment
B) Supportive
C) Selective
D) differential - Answer C) Selective
Which category of media would MacConkey agar best fit?
A) Selective
Questions Fully Answered.
Rejection of inappropriately collected or transported specimens:
A) Is the sole responsibility of the laboratory and should be done without regard to physician requests
B) Should be initiated by laboratory personnel but approved by nursing or physician staff responsible for
the care of the patient
C) Is not in the best interest of patient care and should not be considered appropriate
D) Is the responsibility of nursing staff and should not be an issue for laboratory personnel - Answer B)
Should be initied by laboratory personnel but approved by nursing or physician staff responsible for the
care of the patient
Specimens for mcirobiologic studies should:
A) Be transported to the laboratory within 30 minutes of collection
B) Be contained in specimen containers that are leak-proof
C) SHould be transported within sealable, leak-proof plastc bags with a separate section for paperwork
D) Be clearly marked with a biohazard label
E) All of the above are correct - Answer E) All of the above are correct
Holding media such as Stuart's medium and Amie's medium are used for? - Answer Maintaining the
viability of microorgansims present in a specimen
Direct examinations such as the Gram stain serve what purpose(s)?
A) The quality of the specimen can be assessed
B) To give the physician an early indication of what may be wrong with the patient
C) The workup of the specimen can be guided
D) All of the above are correct - Answer D) All of the above are correct
Which type of media is designed to support the growth of one group of organisms, but not another, by
containing antimicbrobials, dyes, or alcohol? - Answer Selective
,Reduced O2 (5% to 10%) and increased CO2 (8% to 10%) is the environmental condition that best suuits
which type of organism? - Answer Microaerophiles
The agency that reuiqres helath care facilities to provide employees with all devices and mechanisms
necessary to protect them from hazards encountered in the laboratory is? - Answer OSHA
Procedures such as grinding, mincing, vortexing, and preparing smears:
A) Are hazardous and should be done wearing gloves and gown protection
B) Create aerosol droplets and should be performed only in an approved biosafety cabinet
C) Should be avoided with speciments known to contain infectious agents
D) Produce a spill hazard and should be done over an absortive mat
E) All of the above are correct - Answer E) All of the above are correct
The autoclave sterilizes material by: - Answer Applying moist heat at high temperatures and increased
pressure (121 C for 15 minutes at 15 PSI)
In microscopy, resolution can be defined as:
A) The magnification power
B) The extent to which detail in the magnified object is maintained
C) The ability of objects to stand out from the background
D) All of the above are correct - Answer B) The extent to which detail in the magnified object is
maintained
A mordant that is applied after the primary stain to chemically bond the alkaline dye to the bacterial cell
wall is? - Answer Gram's iodine
Which of the following statements are true concerning darkfiel microscopy?
A) This type of microscopy is used in some laboratories to detect spirochetes
B) The condenser does not allow light to pass directly through the specimen but directs the light t ohit
the specimen at an oblique angle
, C) THis technique involves the alteration of microscopic technique rather than the use of dyes or stains
to achieve contrast
D) All of the above are correct - Answer D) All of the above are correct
Organisms that have relatively complex nutritional needs, requiring extra media componenets to be used
for growth, are called? - Answer Fastidious
A bacterial colony can be described as:
A) Sufficiently large numbers of a bacterium that can be observable with the unaided eye
B) Derived from a single bacterial cell
C) Belonging to the same genus and species
D) Having identical genetic and phenotypic characteristics
E) All of the above are correct - Answer E) All of the above are correct
Which category of media can be described as containing nutrients that support growth of most
nonfastidious organisms without giving any particular organism as growth advantage?
A) Enrichment
B) Supportive
C) Selective
D) Differential - Answer B) Supportive
Which category of media can be described as containing one or more agents that are inhibitory to all
organisms except those being sought?
A) Enrichment
B) Supportive
C) Selective
D) differential - Answer C) Selective
Which category of media would MacConkey agar best fit?
A) Selective
