Culture Media: Questions With Verified Answers 2024 Latest Upload

Culture Media: Questions With Verified Answers 2024 Latest Upload what is culture medium? -Correct answer - an artificial environment in the lab that supplies the nutrients and requirements (nutrients, source of energy, certain environmental conditions) needed for microorganisms to grow and reproduce chemically defined vs. complex media: -Correct answer - chemically defined media contains exact amounts of pure chemicals and are used in high-level research - complex media are varied mixtures of nutrients used to support the growth of microbes in our lab activities. general purpose media: -Correct answer - supports a wide variety of microbial growth - ex: nutrient broth (clear test tube= sterile, cloudy test tube= bacterial growth) and nutrient agar - standard nutrients support the growth of most microbes enriched media: -Correct answer - contains extra dietary nutrients that supports the growth of fastidious microbes (any organism that has complex or particular nutritional requirements) - ex: blood agar selective media: -Correct answer - chemicals have been added to inhibit the growth of some microbes, but not others - ex: mycological agar (supports the growth of yeast and molds, but INHIBITS the growth of bacteria by containing antibiotics such as gentamicin and chloramphenicol. purple transparent agar) - ex: macconkey agar (supports the growth of gram-negative bacteria, but INHIBITS the growth of gram-positive bacteria by containing bile salts) macconkey agar (why is it selective and differential medium): -Correct answer - its a selective medium because it supports the growth of gram-negative bacteria but it inhibits the growth of gram-positive bacteria by containing bile salts