Molecular Diagnostics Chapter 4 EXAM GRADED A+

Molecular Diagnostics Chapter 4 EXAM GRADED A+ Gel electrophoresis - answerProcedure used to separate and analyze DNA fragments by placing a mixture of DNA fragments at one end of a porous gel and applying an electrical voltage to the gel Is the anode positive or negative? What about the cathode? - answerPositive, negative DNA/RNA are positively/negatively charged? They migrate towards anode or cathode? - answernegatively, anode Do large or small size DNA fragments migrate faster? - answersmall, easier to move through membrane Heteroduplexes - answerA double-stranded nucleic acid containing one or more mismatched (noncomplementary) base pairs. RNA folding on itself when single stranded agarose gel - answera jelly-like slab used to separate molecules on the basis of molecular weight, polysaccharide that comes from seaweed concentration of agarose in a gel determines the size of spaces in the gel. 3% agarose resolution is best for _______bp of DNA. 0.6% is best ________bp of DNA. - answer50-100 bp and 1000, 20,000 Are low concentration agarose gels easily broken? - answeryes Electroendosmosis - answerThis electrophoresis error is caused by cations in the buffer moving in the opposite direction which slows/distorts migration of samples Pulse field gel electrophoresis (PFGE) - answerused to separate especially long strands of DNA by length in order to tell differences among samples. Doe