Herex CONCEPTS 2e Zit 2023:
PARTIM 1
1) You want to analyse the protein protein interactions in some kind of worm and you can use as
much medium en breed as much as you need. You will have to use LC-MS for this, can you propose a
workflow? What are the pitfalls you may need to solve? Wich technique would you use for genome
wide protein-protein interaction analysis?
2) Wat is Edman degradation sequencing? What are some reasons that it is not used as often
anymore?
PARTIM 2
1) Calculate the molecular weight
2) what are the differences between PMF and PFF? Can you combine the two?
3) Explain TOF and what are some improvements made for better accuracy?
4) What is an imonion ion? How is it formed and Draw the structure?
PARTIM 3
1) They used a Fisher scientific Q exacative, this is a quadrupole connected to an orbitrap. What
would happens with the results if we used a linear ion trap connected to an orbitrap?
2) what is meant by "using an protein enrichment analysis tool?
PARTIM 1
1) You want to analyse the protein protein interactions in some kind of worm and you can use as
much medium en breed as much as you need. You will have to use LC-MS for this, can you propose a
workflow? What are the pitfalls you may need to solve? Wich technique would you use for genome
wide protein-protein interaction analysis?
2) Wat is Edman degradation sequencing? What are some reasons that it is not used as often
anymore?
PARTIM 2
1) Calculate the molecular weight
2) what are the differences between PMF and PFF? Can you combine the two?
3) Explain TOF and what are some improvements made for better accuracy?
4) What is an imonion ion? How is it formed and Draw the structure?
PARTIM 3
1) They used a Fisher scientific Q exacative, this is a quadrupole connected to an orbitrap. What
would happens with the results if we used a linear ion trap connected to an orbitrap?
2) what is meant by "using an protein enrichment analysis tool?
