Transcriptome Guides d'étude, Notes de cours & Résumés

a. Explain what is a transcriptome b. Differentiate between transient and stable transfection/transformation c. Differentiate between constitutive expression, spatial expression and temporal expression d. Explain what a microarray is e. Explain how a microarray can be used to detect differences in gene expression

Clear explanations on the definitions of genome, transcriptome, proteomes and how they are used. The following topics are discussed: - DNA: structure, and mechanisms - RNA: structure, content, processing - Protein: structure, proteome

These are notes on a series of 15 lectures and 9 hours of lab work about different molecular methods used in biology. It covers a wide range of topics and includes diagrams for almost all the processes. The title of each topic is as follows: 1. Genes 2. Plasmid cloning in E.coli 3. Cloning in E. coli - other vector systems 4 - 6. Male sterility via genetic engineering 7.-8. Oligonucleotides and primer-based methods 9. Expressing proteins in E. coli 10. Transformation of eukaryotes 1: Yeast,...