AUA Med 1 -- Biochemistry--
Biotechniques
what is the purpose of Polymerase chain reaction (PCR)? - answer diagnostic tool to
amplify a desired DNA fragment.
What do you need for PCR? - answer Excess PRIMERS, Excess dNTPs, DNA
polymerase (taq)
What is the purpose of DNA polymerase? - answer makes polymers of any molecules
what is a chain reaction ? - answer a reaction that progress in an exponential way
2--4--6--8--10
why making copied of DNA ? - answer diagnosing infections, crime investigations,
molecular biology, genetic research
what is the purpose of primers? - answer needed to start the reaction , help identifying
the portion of amplification
What is the Process of PCR ? - answerdenaturing , annealing , elongation
What is denaturing ? - answerHeating the DNA to 95 to separate the strands
What is annealing ? - answerCooling the sample to 55 so that the primers bind to their
target sequences
what is elongation? - answerraising the temp to 72 so that the taq polymerase attach
the dNTP's to replicate the sequence after each primer
What is CRISPR-Cas9? - answer(cut and paste to edit DNA) Palindromic, cas9 cuts
(cleaves) the DNA , Guide RNA (gRNA) recognize the sequence that need to be edited
what is the meaning of blotting ? - answerdetecting
What are the 3 blotting procedures? - answerSouthern, Northern , Western
What is the blotting mnemonic - answerSNoW DRoP
Southern = DNA
Northern = RNA
Western =Protein
Biotechniques
what is the purpose of Polymerase chain reaction (PCR)? - answer diagnostic tool to
amplify a desired DNA fragment.
What do you need for PCR? - answer Excess PRIMERS, Excess dNTPs, DNA
polymerase (taq)
What is the purpose of DNA polymerase? - answer makes polymers of any molecules
what is a chain reaction ? - answer a reaction that progress in an exponential way
2--4--6--8--10
why making copied of DNA ? - answer diagnosing infections, crime investigations,
molecular biology, genetic research
what is the purpose of primers? - answer needed to start the reaction , help identifying
the portion of amplification
What is the Process of PCR ? - answerdenaturing , annealing , elongation
What is denaturing ? - answerHeating the DNA to 95 to separate the strands
What is annealing ? - answerCooling the sample to 55 so that the primers bind to their
target sequences
what is elongation? - answerraising the temp to 72 so that the taq polymerase attach
the dNTP's to replicate the sequence after each primer
What is CRISPR-Cas9? - answer(cut and paste to edit DNA) Palindromic, cas9 cuts
(cleaves) the DNA , Guide RNA (gRNA) recognize the sequence that need to be edited
what is the meaning of blotting ? - answerdetecting
What are the 3 blotting procedures? - answerSouthern, Northern , Western
What is the blotting mnemonic - answerSNoW DRoP
Southern = DNA
Northern = RNA
Western =Protein
