Samenvatting - Alle microscopen Biomedical Imaging (1080FBDBMW)

Soorten microscopen
Resolution eye = 0,1mm

Microscope Resolution
Limit
Compound Bright Field Microscope 1-10nm
Epifluorescence microscope 200nm
 With Ab 1-10nm
Multiphoton microscopy (3photons) 800µm
Near field scanning optical 20nm
microscope
 Indirect way (TIRF) 100nm
Far field scanning optical microscope 100nm
(SIM)
Transmission Electron Microscopy 0,1nm
(TEM)
Scanning Electron Microscopy (SEM) 1-10nm
 FIB-SEM 1nm

, Compound microscopy
Algemeen:

 Bright field microscope
 Resolution limit: 1-10nm
 Soort
o Upright microscope
 Objective above the sample
o Inverted microscope
 Objective below the sample (look at the cells from
below)
 Mostly 2 lenses: objective and ocular
 ‘Anatomy’
o Light – condenser – sample – objective – ocular – eye
 Conjugate planes
o Lamp filament
o Field diafragm
o Between objective and ocular
o Lens of the eye



Dark field microscopy
Algemeen:

 Dark field
o No sample: Light in very inclined angle (contender annulus)
through the sample  light not be captured by objective
o Sample: Only capture scattered light
 Zernike phase contrast:
o Condenser annulus  causes light to fall outside of the
objective
o Phase plate  shifts the phase os unrefracted light  allow
interference