Tests for Gram-negative bacteria Question and answer rated A+

Tests for Gram-negative bacteria Question and answer rated A+Name the three tests we did in lab IMViC test, TSI test, and Urea test What does the acronym IMViC stand for? Indole Methyl red Voges-Proskauer Citrate When performed together, what family of bacteria do the four tests of the IMViC test differentiate? Enterobacteriaceae Name four major bacteria in the family Enterobacteriaceae 1. Escherichia coli 2. Enterobacter aerogenes 3. Proteus vulgaris 4. Serratia marcescens The Indole test examines what ability of bacteria? hydrolysis of tryptophan by tryptophanase What is produced by when tryptophan is hydrolyzed? indole, pyruvic acid, and ammonia What substance do you test for when determining if the bacteria have hydrolyzed tryptophan? indole What reagent is added in the Indole test to test for the presence of indole? Kovac's reagent What does a positive result of the indole test look like? Immediately turns red at the top When Kovac's reagent combines with indole, what dye is produced and what is its color? Rosindole; red If Kovac's reagent is mistakenly mixed into the indole media, what color does it turn? Can the test be salvaged? green; yes