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Rutgers Intro Biochem 301 -Murphy Module 1 Solutions Determine the pKa for the following solution of acid containing [0.035] A- and [0.1] HA. The pH of the solution is 6.00 4.10 4.30 5.42 6.46 6.64 - Answers6.46 A biochemical reaction will proceed in the direction as written if: ΔG < 0. ΔH < 0. ΔG > 0. ΔG = zero. ΔH > 0. - AnswersΔG < 0. The following molecule is an example of which class of compounds?CH3CH2CH2COOH Hydrocarbon Acid Alcohol Aldehy...

Intro to Biochemistry (Murphy 301) CH 1 – 4 Solutions The side chain of histidine has a typical pKa value in the range of 6.5-7.4. However, when analyzing the pKa values in a particular protein, scientists determined that one particular His residue has an unusually low pKa value of 4.8. Which of the following statements correctly explain this anomaly? 1) The microenvironment around a residue can impact its pKa value. 2) A positively charged amino acid must be in close proximity to t...

Biochemistry 301 UW Madison: Exam 2b Solutions great bulk of lipids in organisms are... - Answerstriglycerides or triglycerols general structure of a fatty acid - Answerscarbon chain + carboxyl group on end source of triglycerides - Answersthe diet, de novo biosynthesis in the liver, and storage deposits in adipocytes Bile salts - Answers- cholesterol derivatice - detergent substances synthesized by the liver - stored in gallbladder - essential for digestion of lipids and their ...

Biochemistry 301 - Exam 3 MCQs and Answers "Mad Hatters" were mad because of what? - Answersheavy metal poisoning Using the "E1, E2, E3" terminology for Pyruvate DH Complex - TPP is found at the active site of which enzyme? - AnswersE1 If carbon one (the carboxyl carbon) of Pyruvate is labeled with carbon-14, and then the Pyruvate is put through one "turn" of the Citric Acid Cycle, which carbon(s) of Oxaloacetate will be labeled? Pick as many choices as are necessar...

Biochem 301-Exam 1 and Answers ionic bond - Answersdirect interaction between positive and negative ions covalent bond - AnswersA chemical bond that involves sharing a pair of electrons between atoms in a molecule Electronegativity - Answersmeasure of how strongly atoms attract bonding electrons to themselves molecular weight - Answersratio of the average mass of one molecule of an element or compound to one twelfth of the mass of an atom of carbon-12 (remember that this is a dimensi...

Biochemistry 301 Exam 3 and Answers What is the function of the citric acid cycle? - AnswersHarvest high energy electrons from carbon fuels What product is found by the condensation of oxaloacetate and acetyl CoA? - AnswersCitrate What makes up citrate? - Answers- Acetyl CoA - Oxaloacetate What is the intermediate between citrate and isocitrate? - AnswersCis-aconitate Where is succinate dehydrogenase located? - AnswersInner membrane of mitochondria What intermediate in the ci...

Biochem 301 Exam 1 and Answers 2nd law of thermodynamics - Answers-nature tends to become more distorted -entropy does not decrease in a closed system (entropy will stay the same or increase) What is the relationship between deltaG and K (equilibrium constant)? - AnswersAs K increases, deltaG decreases. Relationship between K and Q? - AnswersQ < K --> reactions proceeds forwards Q = K --> equilibrium Q > K --> reactions proceeds backwards Other amino acids - Answers...

BIOC 301 - Xmas Exam and Answers What is the goal of PCR? - AnswersArtificially replicate the process of natural DNA replication to generate (amplify) many copies of the piece of DNA of interest What components are needed to do a successful PCR? What do the components do? - Answers- Add heat to break hydrogen bonds binding dsDNA, turns into ssDNA (also EtNa lysis) -Polymerase (Taq) reads DNA strand 5' to 3' for the assembly of complementary strands dNTPs -Primers which allow polyme...

BIOC 301 UBC Final Exam and Answers What type of matrix can be used for Ion Exchange Chromatography? - AnswersInsoluble matter (beads) containing covalently bound charged residues Anion exchange chromatography - AnswersNegative proteins stick to positive beads, only positive proteins go through Cation Exchange Chromatography - AnswersPositive proteins stick to negative beads, only negative proteins go through How do we choose a matrix based on the properties of the protein to be isol...

BIOC 301 Midterm Exam and Answers B-galactosidase lactose galactose glucose - AnswersLacZ codes for ________ which hydrolyzes _______ into ______ and_____ chaotropes - Answerssmall molecules used to denature proteins (urea, guanidinium chloride); bind to water and reduce hydrophobic effect Melting temperature formula will be given if we need it, but how do you calculate the annealing temperature if you have the melting temperature - Answerssubtract between 2-5 degrees from the meltin...