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The laboratory receives a synovial fluid for acid fast culture. How should the tech proceed with this culture? a) Digest the sample for culture b) Centrifuge the specimen and directly inoculate the culture c) Perform a direct smear only d) Reject the specimen Centrifuge the specimen and directly inoculate the culture Body fluids do not have normal flora like respiratory specimens so they don't need to be digested prior. For body fluids: spin and use sediment to inoculate smears and cult...

Based on the indirect immunofluoresence pattern performed on serum, what would be the most appropriate antibody follow-up test? Centromere The flow cytometric results gated on peripheral blood lymphocytes are most consistent with what disease state? CD19 = 68 and CD20 = 72, CD5= 97 B-cell chronic lymphocytic leukemia (CLL) Mechanisms involved in the complement cascade include: opsonization and inflammation

Insufficient centrifugation will result in: A. A false increase in hematocrit (Hct) value B. A false decrease in Hct value C. No effect on Hct value D. All of these options, depending on the patient A. Insufficient centrifugation does not pack down the red blood cells; therefore, the Hct, which is the volume of packed cells, will increase. Variation in red cell size observed on the peripheral smear is described as: A. Anisocytosis B. Hypochromia C. Poikilocytosis D. Pleocytosis A. A...

B. Antigen excess Soluble immune complexes are formed under the condition of: A. antigen deficiency B. antigen excess C. antibody excess D. complement C. Cytotoxic T lymphocytes that possess the CD8 surface marker mediate which of the following T-cell functions? A. delayed type hypersensitivity B. regulatory C. cytotoxic D. helper

% Transferrin Saturation % Transferrin Saturation = Serum Fe/TIBC x 100 Normal ranges: 15 -50% Unconjugated bilirubin (indirect) Indirect Bilirubin ID = Total Bilirubin (TB) - Direct Bilirubin (DB) Normal ranges: TB: 0.2 - 1.2mg/dl DB: <0.3mg/dL IB: 0.2 - 1.2mg/dL

Dilution Formula Sample Volume / (Sample Volume + Diluent Volume) Sensitivity True Positives / (True Positives + False Negatives) Specificity True Negatives / (True Negatives + False Positives)

Dilution Formula Sample Volume / (Sample Volume + Diluent Volume) Sensitivity True Positives / (True Positives + False Negatives) Specificity True Negatives / (True Negatives + False Positives)

Sample Volume / (Sample Volume + Diluent Volume) Dilution Formula True Positives / (True Positives + False Negatives) Sensitivity Equation True Negatives / (True Negatives + False Positives) Specificity Equation

Dilution Formula Sample Volume / (Sample Volume + Diluent Volume) Sensitivity True Positives / (True Positives + False Negatives) Specificity True Negatives / (True Negatives + False Positives)

Sample Volume / (Sample Volume + Diluent Volume) Dilution Formula True Positives / (True Positives + False Negatives) Sensitivity Equation True Negatives / (True Negatives + False Positives) Specificity Equation

CA-125 ovarian cancer tumor marker PSA, PAP prostate tumor marker CA-15-3 Breast Cancer Tumor Marker

What feature is most often found with patients with a splenectomy on a Wright's stained blood smear? Howell-Jolly bodies Patients with antibody to the following antigen are immune to Hepatitis B: Surface antigen (HbSAg) Which two of the following tests are helpful for documenting previous Streptococcal throat and skin infections: ASO titer and Anti-DNase B

CA-125 ovarian cancer tumor marker PSA, PAP prostate tumor marker CA-15-3 Breast Cancer Tumor Marker

What feature is most often found with patients with a splenectomy on a Wright's stained blood smear? Howell-Jolly bodies Patients with antibody to the following antigen are immune to Hepatitis B: Surface antigen (HbSAg) Which two of the following tests are helpful for documenting previous Streptococcal throat and skin infections: ASO titer and Anti-DNase B

The weight of NaCl present in 650 ml of a 0.85% NaCl solution is: Weight (in grams) of substance in a quantity of solution = (total ml volume / 100 mL) x (percent concentration) So: Weight = (650 mL / 100 mL) x (0.85%) = 5.525 grams Proficiency Testing Service (e.g., CAP) reported the following results for a Cholesterol test (unknown Control) reported by your laboratory. Your results were compared with 648 other labs using the same type instrumentation and methodology: Your Result = 168 m...

The light-colored zone adjacent to the nucleus in a plasmacyte is the: A. ribosome B. chromatin C. mitochondria D. Golgi area D. Golgi area The majority of the iron in an adult is found as a constituent of: A. hemoglobin B. hemosiderin C. myoglobin D. transferrin A. hemoglobin

Which of the following instruments measures changes in vapor pressure? Osmometer What abnormal red blood cell inclusion is composed of multiple small, dark blue aggregates of ribosomes (RNA) that are distributed throughout the RBC? Basophilic stippling A situation may arise when a pathologist recognizes the presence of a parasite in an H & E - stained section within the lumen of the appendix, as seen in the upper Image. Studies of additional fields of view in the slide may locate the ovar...

Which of the following instruments measures changes in vapor pressure? Osmometer What abnormal red blood cell inclusion is composed of multiple small, dark blue aggregates of ribosomes (RNA) that are distributed throughout the RBC? Basophilic stippling A situation may arise when a pathologist recognizes the presence of a parasite in an H & E - stained section within the lumen of the appendix, as seen in the upper Image. Studies of additional fields of view in the slide may locate the ovar...

Which is required to generate cDNA A. DNA polymerase B. RNase H C. Restriction Enzyme D. Helicase B What is the function of Taq Polymerase recognize dsDNA

CML vs. Leukemoid Reaction CML - decreased leukocyte alkaline phosphatase (LAP) Leukemoid Reaction - increased LAP Look-back The process for the identification and notification of recipients of blood components from prior donations of who now test positive for HCV, HIV-1 and/or HIV-2. External reference electrode used in ISE KC