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Lab6:CarbohydrateFermentation LearningObjectives: • Definefermentationanddevelopaknowledgeoffermentationendproducts. • Understandthetheorybehindthecarbohydratefermentationprocedure. • Understandthevariouscomponentsofthecarbohydratemediaandhowthesecomponents work. • Appropriatelyanalyzeresultsforavarietyofmicroorganisms. Intheclinicallaboratory,onetechniqueusedtoidentifybacteriaisthecarbohydratefermentationtests.Bacteriahavetheabilitytoutilizedifferenttypesofcarbohydrates;ifthebact...

Lab5:DifferentialStaining LearningObjectives: • Explaintheprincipleofdifferentialstaining • Applydifferentialstainingtoidentifybacteria Differentialstainusestwoormorestainstospecificallystaincertainstructuresorcellularcomponentswhichcannotbeeasilyobservedusingsimplestains.Differential staining principles are based upon the specific chemical nature and composition of cellular components and therefore, different structures areobservedusingdifferentstainsandstainingprocedures.Differenti...

MICRO. Exam 1 1. Question:Describe the idea of spontaneous generation and what experiments have refuted this idea? Describe the experiment of Louis Pasteur in detail as well as the inferences that were made. What was a specific feature of the experiment that has allowed to experiment? 2. Question:Postulates of Robert Koch. List them all and their significance in microbiology. 3. Question:Review the contribution of each scientist in the field of Microbiology: John Snow, Alexander Fleming, Igna...

Exam1review WEEK1 HereissomeinformationaboutExam1. • Thisexaminationcoverscontentlearnedinweeks1and2. • Thisexamisworth120totalpoints,andincludesthefollowingtypesofquestions: o 25Multiplechoicequestions o 5Fillintheblankquestions o 2Shortanswerquestions o 2Essayquestions • Youhave90minutestocompletetheExam. • Whenthetimelimitisreached,youwillautomaticallybeexitedfromtheexam. • Remembertochoosethebestanswer,somequestionsmayhavemorethanonecorrectanswerbutonly one BESTanswe...

Required Resources Read/review the following resources for this activity: • Textbook: Chapters 7, 8 • Weekly Concepts Initial Post Instructions Class, let’s discuss the categories that organisms can be grouped in based on their nutritional requirements. Find one microorganism, either a prokaryote or eukaryote, and describe the environment in which it lives. (Does it live underwater? On skin? In soil? Give as many details as possible!) To complete your initial post, you will then use...

Required Resources Read/review the following resources for this activity: • Textbook: Chapters 7, 8 • Weekly Concepts Initial Post Instructions Class, let’s discuss the categories that organisms can be grouped in based on their nutritional requirements. Find one microorganism, either a prokaryote or eukaryote, and describe the environment in which it lives. (Does it live underwater? On skin? In soil? Give as many details as possible!) To complete your initial post, you will then use t...

Week3Concepts:Metabolism FactorsaffectingMicrobialGrowth Prepare:FactorsAffectingMicrobialGrowth • Thephaseofthebacterialgrowthcurveinwhichtherateofmultiplicationequalstherateofcelldeathisthe .deathphase • AnorganismthatgrowsinlowerpHconditionsiscalleda . • Anorganismthatusesorganiccarbonforitscarbonneedsandsunlightforitsenergyneedswouldbecalleda . Self-Check:GrowthRequirementsinMicroorganisms • Place the images in order from top to bottom to show extracellular digestion in ba...

WK3ALTMicrobialNutrition 1. Theorganismsthatcanuseinorganiccarbonandtheenergyofchemicalreactions ______ . 2. A______requiresorganicmoleculesforcarbonandenergyfromsunlight. 3. During______transportwatertransfersthroughsemipermeablefollowing concentration gradient. 4. Microorganismslivinginsoilandwaterassociatedwithvolcanicactivity,compostpiles,habitatsdirectlyexposedtothesunare______. 5. In______transportnutrientsaretransportedagainstthediffusiongradientorinthesamedirectionasthenaturalgrad...

Lab4:SimpleStaining LearningObjectives: • Tolearnaboutprincipleofstaining • Tolearntheprocessofsimplestain • Topreparewetmountslides Exercise1:SimpleStainMaterials: OvernightculturesofS.epidermidis,B.subtilis,S.cerevisiae(yeast),inoculatingloop,glassslides,incinerators,crystalvioletoranyotherstainchosenbyinstructor;tongs,stainingracks/trays,immersionoil,lenspaper,bibulouspaper,microscope Method: 1. Obtainacleanglassslide,loopandovernightcultures.Holdtheglassslidesfromtheedgestoav...

Lab3:Microscopy-ObservationofCells LearningObjectives: • Usethecompoundlightmicroscopeforobservationofcells. • Identifydifferenttypesofbacteriabasedontheirshapes. • Identifyavarietyofeukaryoticcells. Introduction: Exercise2:ObservationofDifferentTypesofBacteriaMaterials: Compoundmicroscope,immersionoil,lenswipes,preparedslidesofvariousbacteria-Rods,cocciandspiral Method: 1. Obtainthepreservedslideofabacteria. 2. UseAppendixAandfollowtheinstructionsonhowtouseaMicroscope. 3. O...

BIOS 242 Week 2 Discussion: Prokaryotes and Eukaryotes Choose ONE cellular component that is only found in eukaryotes. Discuss what its role is.

Week2Concepts:ClassificationandNutrition ProkaryoticvsEukaryoticCells Prepare:ProkaryoticvsEukaryoticCells • Thecellularstructuresfoundinprokaryotesare(selectallthatapply) • TheGramstainworksbecauseofdifferencesinthe ofbacteria. Prokaryotesinclude • Which of the following structures contribute to pathogenicity of bacteria (select all that apply) Self-Check: Bacteria andArchaea • Drag the cell types to the three respective highlighted images at the bottom. Self-Check:BacteriaExte...

1. DrawanimageofaProkaryoticcellinthespacebelow.Labelalltheorganellesandstructurespresentin atypicalprokaryotic cell. 2. CompletethefollowingtablebylistingvariousorganellesandstructurescommonlyfoundinaProkaryoticcellanddescribe theirfunctionsin 1-2sentences. 3. DrawanimageofaEukaryoticcellinthespacebelow.Labelalltheorganellesandstructurespresentin atypicalEukaryotic cell. 4. CompletethefollowingtablebylistingvariousorganellesandstructurescommonlyfoundinaEukaryoticcell and describetheirfunctio...

Lab1:CultureTransferTechniques LearningObjectives: • Identifytheimportanceofaseptictechniqueinthefieldofmicrobiology • Applytheconceptofaseptictechniqueanditsimportanceinthefieldofmicrobiology. • Identifydifferentformsofbasicgrowthmedia • Transferapurebacterialculturefromonegrowthmediatoanother,aprocesscalledsub-culturing. Introduction: Materials: Nutrientbroth,Nutrientagarslants,Nutrientagarstabs,liquidandslantculturesofSerratiamarcescens, inoculatingloop,inoculatingneedle,in...

Prepare:HistoryofMicrobiology • Doesnothavemembraneboundcellularorganellesandhascellwallmadeupofpeptidoglycan: • AccordingtoBinomialsystemthescientificnameofanylivingorganismhas(selectallthatapply): • Hewasthefirstpersontouseswanneckflasktodisapprovethetheoryofspontaneousgeneration: • FatherofMicrobiologywhocalledmicrobesanimalcules: • Whichfieldofmicrobiologymonitorsandcontrolsthespreadofdiseasesincommunities? Self-Check:Microorganisms VirusesbelongtotheAcellularcategoryofmic...

BIOS 242 Week 1 Assignment; Golden Age and Macromolecules

BIOS 242 Lab Practicum Review

Lab2:TechniquesforIsolationofPureCultures LearningObjectives: • Isolatepureculturesfromamixedpopulationofmicroorganismsusingaseptictechnique. • Learnthestreakplatemethod Introduction: Inthislaboratoryexercise,coloniesfrommixturesofEscherichiacoliandSerratiamarcescenswillbeisolatedusingthefour-waystreakmethodassummarizedbelow(Figure1). We will need this image from Materials: A24hournutrientbrothmixtureofSerratiamarsecens(S.marsecens)andEscherichiacoli(E.coli),twonutrientagarpl...