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Examen
BCH5413 TEST 2 QUESTIONS AND ANSWERS ALL REVISED AND UPDATED
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---111abril 20252024/2025A+
- Stages of transcription initiation - ANSWER -- holoenzyme recognizes 
regulatory sequence in promoter and binds forming the closed promoter 
complex - unwind dsDNA to form open promoter complex - transcription initiates (RNA Pol generates short abortive transcripts 
that are unstable and released) - at 10nt in length, the complex is stable and turns from initiation to 
elongation 
Cycling of sigma during transcription - ANSWER -- as Pol transitions 
to elongation, sigma factor is relea...
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