Experimental Cell Biology 1

Experimental Cell Biology I 2022-2023

This document provides a summary about the basics of cell biology. It is a brief summary about the different cell compartments, such as the cell membrane, nucleus, nucleolus, ER, Golgi apparatus, mitochondria, lysosomes, transport vesicles, cytoskeleton, chloroplasts, the cytosol, ATP, Ion motive forces, Glycolysis, Pyruvate oxidation, Citric acid cycle, Oxidative phosphorylation, Respiratory chain/electron transport chain, ROS formation, ATP as a drug target, ATP in synapses, Warburg effect, P5...

This summary contains information about: RNA polymerase, Intragenic promoters, CpG island promoters, DNA cis-acting elements, histone modifications, epigenetics, enhancers, DNA methylation, and tumours.

This summary contains information about: DNA replication mechanisms, DNA polymerase III proofreading, strand-directed mismatch repair, post-replication DNA repair, base excision repair, mutations, nucleotide exchange repair, double-strand break repair, nonhomologous end joining, homologous recombination, RecA, Rad51, Rad52, molecular chaperones, HSP70, HSP60, proteasomes, ubiquitin, amyloid formation, heat-shock response, and HSF1.

This summary contains information about: the synaptic vesicle cycle, transmission electron microscopy, BoTX, literature research, and types of fixation (chemical vs. cyro-fixation).

This summary contains information about the following subjects: membranes, sphingolipids, glycolipids, phospholipids, bilayers, membrane proteins, integral membrane proteins, peripheral membrane proteins, folding of membrane proteins, beta-barrels, glycosylation of membranes, detergents, nanodiscs, signal sequences, targetting sequences, secretory pathway, signal peptides, chaperones, membrane translocation, SRP, protein targetting and secretion, and biogenesis of membrane proteins.

This summary contains information about endosomes, endosome maturation, Rab5, Rab7, PI(3)P, sorting nexins, SNX-BAR domains, research/literature about the formation of tubules in the membrane, membrane curvature, liposome remodelling assay, retromers, and Alzheimer’s.