PMT
Please wrîte clearly în block capîtals.
Centre number Candîdate number
Surname
Forename(s)
Candîdate sîgnature
Î declare thîs îs my own work.
A-level
BÎOLOGY
Paper 2
Frîday 16 June 2026 Mornîng Tîme allowed: 2 hours
Materîals For Examîner’s Use
For thîs paper you must have:
• a ruler wîth mîllîmetre measurements Questîon Mark
• a scîentîfîc calculator. 1
2
Înstructîons
• Use black înk or black ball-poînt pen. 3
• Fîll în the boxes at the top of thîs page. 4
• Answer all questîons. 5
• You must answer the questîons în the spaces provîded. Do not wrîte
outsîde the box around each page or on blank pages. 6
• Îf you need extra space for your answer(s), use the lîned pages at the end of 7
thîs book. Wrîte the questîon number agaînst your answer(s). 8
• Show all your workîng.
9
• Do all rough work în thîs book. Cross through any work you do not want
to be marked. 10
TOTAL
Înformatîon
• The marks for the questîons are shown în brackets.
• The maxîmum mark for thîs paper îs 91.
ÎB/M/Jun23/E12 7402/2
, PMT
2
Do not wrîte
outsîde the
Answer all questîons în the spaces provîded. box
0 1 . 1 A student used chromatography to separate the dîfferent photosynthetîc pîgments în a
chlorophyll solutîon. She had the followîng materîals.
Chromatography paper
A ruler and pencîl
Suîtable glassware
A solvent (solvent A)
2 cm3 of the chlorophyll solutîon
Descrîbe how she could use these materîals to separate the photosynthetîc pîgments
by chromatography.
[4 marks]
0 1 . 2 Usîng solvent A, the student separated fîve pîgments. She then repeated her method
usîng a dîfferent solvent, B. Usîng solvent B, she separated sîx pîgments.
Explaîn the dîfference between these results.
[2 marks]
6
ÎB/M/Jun23/7402/2
, PMT
3
Do not wrîte
outsîde the
0 2 . 1 Glutamate îs a neurotransmîtter învolved în the transmîssîon of nerve împulses from box
paîn receptors to the braîn. Zîconotîde îs a drug that can reduce severe, constant
paîn. Zîconotîde blocks the calcîum îon channels at some of the synapses whîch use
glutamate.
The transmîssîon of glutamate at synapses îs sîmîlar to that of acetylcholîne.
Explaîn how zîconotîde reduces severe, constant paîn.
[5 marks]
Zîconotîde îs a polypeptîde and acts on synapses în the spînal cord. Scîentîsts
învestîgated the effectîveness of zîconotîde în reducîng severe, constant paîn.
Zîconotîde was înjected înto each patîent’s cerebrospînal fluîd that bathes the braîn
and spînal cord. Patîents recorded the întensîty of theîr paîn usîng a statîstîcally
valîd scale.
0 2
. 2 Suggest two reasons why the patîents had zîconotîde înjected înto theîr cerebrospînal
fluîd rather than takîng a pîll contaînîng the drug.
[2 marks]
1
2
Questîon 2 contînues on the next page
Turn over ►
ÎB/M/Jun23/7402/2
, PMT
4
Do not wrîte
outsîde the
0 2 . 3 Zîconotîde was înjected at 3 ng kg–1 h–1 for 8 days înto each patîent. box
1 ng = 1 × 10–9 g
Calculate the total mass în grams of zîconotîde înjected after 8 days înto a patîent
wîth a body mass of 82 kg
Show your workîng.
[2 marks]
Answer g
0 2 . 4 When the patîents recorded the întensîty of paîn, suggest two reasons why ît was
împortant to use a statîstîcally valîd scale.
[2 marks]
1
2
11
ÎB/M/Jun23/7402/2
Please wrîte clearly în block capîtals.
Centre number Candîdate number
Surname
Forename(s)
Candîdate sîgnature
Î declare thîs îs my own work.
A-level
BÎOLOGY
Paper 2
Frîday 16 June 2026 Mornîng Tîme allowed: 2 hours
Materîals For Examîner’s Use
For thîs paper you must have:
• a ruler wîth mîllîmetre measurements Questîon Mark
• a scîentîfîc calculator. 1
2
Înstructîons
• Use black înk or black ball-poînt pen. 3
• Fîll în the boxes at the top of thîs page. 4
• Answer all questîons. 5
• You must answer the questîons în the spaces provîded. Do not wrîte
outsîde the box around each page or on blank pages. 6
• Îf you need extra space for your answer(s), use the lîned pages at the end of 7
thîs book. Wrîte the questîon number agaînst your answer(s). 8
• Show all your workîng.
9
• Do all rough work în thîs book. Cross through any work you do not want
to be marked. 10
TOTAL
Înformatîon
• The marks for the questîons are shown în brackets.
• The maxîmum mark for thîs paper îs 91.
ÎB/M/Jun23/E12 7402/2
, PMT
2
Do not wrîte
outsîde the
Answer all questîons în the spaces provîded. box
0 1 . 1 A student used chromatography to separate the dîfferent photosynthetîc pîgments în a
chlorophyll solutîon. She had the followîng materîals.
Chromatography paper
A ruler and pencîl
Suîtable glassware
A solvent (solvent A)
2 cm3 of the chlorophyll solutîon
Descrîbe how she could use these materîals to separate the photosynthetîc pîgments
by chromatography.
[4 marks]
0 1 . 2 Usîng solvent A, the student separated fîve pîgments. She then repeated her method
usîng a dîfferent solvent, B. Usîng solvent B, she separated sîx pîgments.
Explaîn the dîfference between these results.
[2 marks]
6
ÎB/M/Jun23/7402/2
, PMT
3
Do not wrîte
outsîde the
0 2 . 1 Glutamate îs a neurotransmîtter învolved în the transmîssîon of nerve împulses from box
paîn receptors to the braîn. Zîconotîde îs a drug that can reduce severe, constant
paîn. Zîconotîde blocks the calcîum îon channels at some of the synapses whîch use
glutamate.
The transmîssîon of glutamate at synapses îs sîmîlar to that of acetylcholîne.
Explaîn how zîconotîde reduces severe, constant paîn.
[5 marks]
Zîconotîde îs a polypeptîde and acts on synapses în the spînal cord. Scîentîsts
învestîgated the effectîveness of zîconotîde în reducîng severe, constant paîn.
Zîconotîde was înjected înto each patîent’s cerebrospînal fluîd that bathes the braîn
and spînal cord. Patîents recorded the întensîty of theîr paîn usîng a statîstîcally
valîd scale.
0 2
. 2 Suggest two reasons why the patîents had zîconotîde înjected înto theîr cerebrospînal
fluîd rather than takîng a pîll contaînîng the drug.
[2 marks]
1
2
Questîon 2 contînues on the next page
Turn over ►
ÎB/M/Jun23/7402/2
, PMT
4
Do not wrîte
outsîde the
0 2 . 3 Zîconotîde was înjected at 3 ng kg–1 h–1 for 8 days înto each patîent. box
1 ng = 1 × 10–9 g
Calculate the total mass în grams of zîconotîde înjected after 8 days înto a patîent
wîth a body mass of 82 kg
Show your workîng.
[2 marks]
Answer g
0 2 . 4 When the patîents recorded the întensîty of paîn, suggest two reasons why ît was
împortant to use a statîstîcally valîd scale.
[2 marks]
1
2
11
ÎB/M/Jun23/7402/2
