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micrometer - answer-one millionth of a meter
nanometer - answer-one billionth of a meter
two critical factors that influence our ability to see an object - answer-resolution
and contrast
resolution - answer-the distance between two objects at which the objects still
can be seen as separate
contrast - answer-the difference in light absorbance between two (objects)
the closer two objects are to each other the __________________ the resolution
requirement - answer-greater
the ________________ the contrast between an object and its background, the
harder it will be to see the object - answer-lower
, bright field microscope - answer-simplest form of light, or optical,
microscopy/light, most often emitted from a standard halogen bulb, enters the
microscope from the base (bottom) and is reflected via mirrors towards the
sample
before the light reaches the sample, it first passes through a ________________
converging the light beams into a focused area on the sample - answer-condenser
iris diaphragm - answer-controls the amount of light that passes through the
sample and into the objective lens
objective lens - answer-the lens closest to the sample and yields the greatest
amount of magnification
the degree of magnification is ______________________ to the amount of light
needed/to image samples clearly at higher magnifications, more light is required -
answer-directly proportional
ocular lens - answer-eyepiece
10x - answer-most common power of ocular lens
total magnification - answer-objective x ocular lens=
staining - answer-required due to the limitation of resolution on unstained cells,
(i.e.) often the flat and transparent regions of a cell may appear invisible under
bright field conditions/with various dyes, these regions can become labeled and
thus visualized.